Sensors and Actuators, B: Chemical

, volume 315 , pages 128135

A universal ultrasensitive platform for enzyme-linked immunoassay based on responsive surface-enhanced Raman scattering

Nan Li ^{1, 2, 3, 4}

Haoling Chen ^{1, 2, 3, 4}

Zhang Meiying ^{1, 2, 3, 4}

Yongchao Zha ^{1, 2, 3, 4}

Zongxia Mu ^{1, 2, 3, 4}

Ying Ma ⁵

Peng Chen ⁶

Hide authors affiliations Show authors affiliations: 6 affiliations

Key Laboratory of Biomaterials of Guangdong Higher Education Institutes |

Department of BioMedical Engineering

JINAN UNIVERSITY |

⁴

Guangzhou 510632 China |

⁵

College of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China |

⁶

School of Chemical and Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, 637457, Singapore. |

Publication type: Journal Article

Publication date: 2020-07-01

Elsevier

Sensors and Actuators, B: Chemical

wos Q1

SJR: —

CiteScore: —

Impact factor: 7.7

ISSN: 09254005

DOI: 10.1016/j.snb.2020.128135

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Materials Chemistry

Metals and Alloys

Surfaces, Coatings and Films

Electronic, Optical and Magnetic Materials

Condensed Matter Physics

Electrical and Electronic Engineering

Instrumentation

Abstract

Enzyme-linked immunosorbent assay (ELISA) is the current gold standard assay for biomarkers. However, its detection sensitivity is greatly limited by the colorimetric readout. Herein, we developed a high-performance immunoassay through the combination of traditional ELISA and ultrasensitive surface-enhanced Raman scattering (SERS) assay using a responsive SERS probe. The probe is a Au nanoplate constituted cabbage-like microparticle functionalized with synthesized phenol-responsive Raman reporter 4, 4’-dithiodibenzyldiazonium (DTDBD). The new SERS probe can detect trace amount of phenol with a limit of detection (LOD) of 0.4 nM, and alkaline phosphatase (LOD of 0.04 mU/L) with phenol as an enzymatic product. More important, the ultrasensitive detection of cholera toxin (CT) in real serum samples indicates such ELISA-SERS assay promising for practical applications using alkaline phosphatase as an enzyme labeling and SERS probe as a signal readout. We envision that this new method can be employed as a universal platform for the profiling of any antigen using alkaline phosphatase as the enzymatic label to its corresponding antibody.

Found

1 citation

Zvereva Maria

DSc in Chemistry, professor

102 publications, 1 295 citations

h-index: 19

Lomonosov Moscow State University

1 citation

Le-Deygen Irina

🥼 🤝

PhD in Chemistry

68 publications, 2 744 citations, 8 reviews

h-index: 19

Lomonosov Moscow State University

Research interests

Antibiotics

Chitosan

Drug delivery systems

Polymers for drug delivery

Proteins

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Li N. et al. A universal ultrasensitive platform for enzyme-linked immunoassay based on responsive surface-enhanced Raman scattering // Sensors and Actuators, B: Chemical. 2020. Vol. 315. p. 128135.

GOST all authors (up to 50) Copy

Li N., Chen H., Meiying Z., Zha Y., Mu Z., Ma Y., Chen P. A universal ultrasensitive platform for enzyme-linked immunoassay based on responsive surface-enhanced Raman scattering // Sensors and Actuators, B: Chemical. 2020. Vol. 315. p. 128135.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1016/j.snb.2020.128135

UR - https://doi.org/10.1016/j.snb.2020.128135

TI - A universal ultrasensitive platform for enzyme-linked immunoassay based on responsive surface-enhanced Raman scattering

T2 - Sensors and Actuators, B: Chemical

AU - Li, Nan

AU - Chen, Haoling

AU - Meiying, Zhang

AU - Zha, Yongchao

AU - Mu, Zongxia

AU - Ma, Ying

AU - Chen, Peng

PY - 2020

DA - 2020/07/01

PB - Elsevier

SP - 128135

VL - 315

SN - 0925-4005

ER -

BibTex

Cite this

BibTex (up to 50 authors) Copy

@article{2020_Li,

author = {Nan Li and Haoling Chen and Zhang Meiying and Yongchao Zha and Zongxia Mu and Ying Ma and Peng Chen},

title = {A universal ultrasensitive platform for enzyme-linked immunoassay based on responsive surface-enhanced Raman scattering},

journal = {Sensors and Actuators, B: Chemical},

year = {2020},

volume = {315},

publisher = {Elsevier},

month = {jul},

url = {https://doi.org/10.1016/j.snb.2020.128135},

pages = {128135},

doi = {10.1016/j.snb.2020.128135}

}

Publisher

Elsevier

Journal

Sensors and Actuators, B: Chemical

wos Q1

SJR

—

CiteScore

—

Impact factor

7.7

ISSN

09254005 (Print)