Sensors and Actuators, B: Chemical, volume 367, pages 132067
Direct ultrasensitive electrochemical detection of breast cancer biomarker-miRNA-21 employing an aptasensor based on a microgel nanoparticle composite
Zhiwei Hou
1
,
Jun Zheng
2
,
Chengfang Zhang
3
,
Ting Li
1
,
Die Chen
1
,
Liang Hu
3
,
Junrui Hu
4
,
Bing Xiong
5
,
Huarong Ye
5
,
Zhenzhong Guo
1
2
General Surgery Department, The Fifth Hospital of Wuhan, Wuhan 430050, PR China
|
5
China Resources and Wisco General Hospital, Wuhan 430080, PR China
|
6
University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, Villeurbanne 69100, France
|
Publication type: Journal Article
Publication date: 2022-09-01
Journal:
Sensors and Actuators, B: Chemical
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 8.4
ISSN: 09254005
Materials Chemistry
Metals and Alloys
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Electrical and Electronic Engineering
Instrumentation
Abstract
Breast cancer is a common disease with high morbidity and mortality among women worldwide. Serum miRNA-21 expression is higher in breast cancer patients and can be employed as a predictor for early breast cancer diagnosis. There is an urgent need for a sensitive, convenient and cost-effective biosensor to assist in the direct diagnosis of breast cancer through clinical biomarkers. In this article, a highly sensitive microgel nanocomposite-based electrochemical aptasensor is prepared for the detection of miRNA-21. The microgel particles of gold nanoparticles are wrapped in a mixture of acrylic acid (AAc) and N-isopropylacrylamide (NIPAm) polymerization. This leads to a porous network structure which can provide a highly biocompatible and anti-fouling microenvironment, similar to that of aqueous biological tissue for RNA capture reaction. Complementary to miRNA-21, an amino-modified oligonucleotide chain capture probe is covalently immobilized on the microgel material, while the hybridization of miRNA-21 with the capture probe is monitored by different pulse voltammetry (DPV). The sensing platform can directly and accurately detect target miRNAs in serum without sample preparation and purification. The concentration response of the aptasensor presents a linear range of 10 aM to 1 pM as well as a detection limit of 1.35 aM. The aptasensor also exhibits noticeable selectivity, stability and reproducibility. Simultaneously, we successfully apply this method to the subsequent detection of serum miRNA-21 in 2 breast cancer patients before treatment and after recovery respectively. The consequence proves that the method has promising potential applications in the early diagnosis and treatment of breast cancer patients. • This test utilises a microgel material with a 3D mesh structure, which maintains the biological activity of miRNA. • We successfully applied this method to the follow-up detection of serum miRNA-21 in 2 breast cancer patients. • Up to date, the limit of detection was as low as 1.35amol/L.
Top-30
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Citations by publishers
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Elsevier
11 publications, 44%
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Springer Nature
4 publications, 16%
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3 publications, 12%
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3 publications, 12%
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2 publications, 8%
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Wiley
1 publication, 4%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 4%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Hou Z. et al. Direct ultrasensitive electrochemical detection of breast cancer biomarker-miRNA-21 employing an aptasensor based on a microgel nanoparticle composite // Sensors and Actuators, B: Chemical. 2022. Vol. 367. p. 132067.
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Hou Z., Zheng J., Zhang C., Li T., Chen D., Hu L., Hu J., Xiong B., Ye H., Jaffrezic-Renault N., Guo Z. Direct ultrasensitive electrochemical detection of breast cancer biomarker-miRNA-21 employing an aptasensor based on a microgel nanoparticle composite // Sensors and Actuators, B: Chemical. 2022. Vol. 367. p. 132067.
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TY - JOUR
DO - 10.1016/j.snb.2022.132067
UR - https://doi.org/10.1016/j.snb.2022.132067
TI - Direct ultrasensitive electrochemical detection of breast cancer biomarker-miRNA-21 employing an aptasensor based on a microgel nanoparticle composite
T2 - Sensors and Actuators, B: Chemical
AU - Hou, Zhiwei
AU - Zheng, Jun
AU - Zhang, Chengfang
AU - Li, Ting
AU - Chen, Die
AU - Hu, Liang
AU - Hu, Junrui
AU - Xiong, Bing
AU - Ye, Huarong
AU - Jaffrezic-Renault, Nicole
AU - Guo, Zhenzhong
PY - 2022
DA - 2022/09/01 00:00:00
PB - Elsevier
SP - 132067
VL - 367
SN - 0925-4005
ER -
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@article{2022_Hou,
author = {Zhiwei Hou and Jun Zheng and Chengfang Zhang and Ting Li and Die Chen and Liang Hu and Junrui Hu and Bing Xiong and Huarong Ye and Nicole Jaffrezic-Renault and Zhenzhong Guo},
title = {Direct ultrasensitive electrochemical detection of breast cancer biomarker-miRNA-21 employing an aptasensor based on a microgel nanoparticle composite},
journal = {Sensors and Actuators, B: Chemical},
year = {2022},
volume = {367},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016/j.snb.2022.132067},
pages = {132067},
doi = {10.1016/j.snb.2022.132067}
}
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