Talanta, volume 70, issue 5, pages 1157-1163
Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection
1
Publication type: Journal Article
Publication date: 2006-12-15
Analytical Chemistry
Abstract
A method of capillary electrophoresis with wall-jet amperometric detection (AD) has been developed for separation and determination of l-tyrosine (Tyr) and its metabolites, such as Tyramine (TA), p-hydroxyphenylpyruvic (pHPP), homogentisic acid (HGA) and some dipeptides containing Tyr, such as Tyr-Gly-Gly (YGG), Tyr-Arg (YR) and Tyr-d-Arg (Y-d-R). A carbon disk electrode was used as the working electrode and the optimal detection potential was 1.00V (versus Ag/AgCl). At 18kV of applied voltage, the seven compounds were completely separated within 20min in 110x10(-3)mol/L Na(2)HPO(4)-NaH(2)PO(4) buffer (pH 7.10) containing 3x10(-3)mol/L beta-cyclodextrin (beta-CD). Good linear relationship was obtained for all analytes and the detection limits of seven analytes were in the range of 0.95-4.25ng/mL. The proposed method has been applied to examine the metabolic process of l-tyrosine in rabbit's urine.
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Citations by publishers
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1 publication, 2.13%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Huang Y. et al. Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection // Talanta. 2006. Vol. 70. No. 5. pp. 1157-1163.
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Huang Y., Jiang X., Wang W., Duan J., Chen G. Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection // Talanta. 2006. Vol. 70. No. 5. pp. 1157-1163.
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TY - JOUR
DO - 10.1016/j.talanta.2006.03.009
UR - https://doi.org/10.1016/j.talanta.2006.03.009
TI - Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection
T2 - Talanta
AU - Huang, Ying
AU - Jiang, Xiuyan
AU - Wang, Wei
AU - Duan, Jianping
AU - Chen, Guonan
PY - 2006
DA - 2006/12/15 00:00:00
PB - Elsevier
SP - 1157-1163
IS - 5
VL - 70
SN - 0039-9140
SN - 1873-3573
ER -
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@article{2006_Huang,
author = {Ying Huang and Xiuyan Jiang and Wei Wang and Jianping Duan and Guonan Chen},
title = {Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection},
journal = {Talanta},
year = {2006},
volume = {70},
publisher = {Elsevier},
month = {dec},
url = {https://doi.org/10.1016/j.talanta.2006.03.009},
number = {5},
pages = {1157--1163},
doi = {10.1016/j.talanta.2006.03.009}
}
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Huang, Ying, et al. “Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection.” Talanta, vol. 70, no. 5, Dec. 2006, pp. 1157-1163. https://doi.org/10.1016/j.talanta.2006.03.009.