Talanta, volume 229, pages 122274

Capture-SELEX for aptamer selection: A short review

CHEN LYU ^{1, 2}

Imran Khan

Zhouping Wang ¹

Hide authors affiliations Show authors affiliations: 2 affiliations

State Key laboratory of Food Science and Technology, Jiangnan University, Wuxi, China |

School of Food Science and technology, Jiangnan University, Wuxi, China |

Publication type: Journal Article

Publication date: 2021-07-01

Elsevier

Journal: Talanta

Quartile SCImago

Quartile WOS

Impact factor: 6.1

ISSN: 00399140, 18733573

DOI: 10.1016/j.talanta.2021.122274

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Analytical Chemistry

Abstract

The emerging aptamer, developed through the systematic evolution of ligands by exponential enrichment (SELEX) process, has revolutionized and facilitated the discoveries in basic research. Among all SELEX technology, Capture-SELEX is a variant of the in vitro selection process, which is suitable for isolating aptamers against small molecules. Capture-SELEX library was developed to enable the immobilization of the oligonucleotides instead of the target molecules during the aptamer selection process. The review provides an update on the recent-advances in this new screening method with particular emphasis on key points of capture protocol and its applications. The limitations and the prospects of the Capture-SELEX are also discussed. We hope that present review will inspire more researchers to understand the selection problems from the perspective of Capture-SELEX. Moreover, it will open new pave to improve the efficiency and success of screening to meet the growing demand for aptasensor discovery in small molecules. • This paper describes the principle and the process of Capture-SELEX. • The design of key elements in the Capture-SELEX screening process are introduced. • Aptamers developed by Capture-SELEX for food safety monitoring are reviewed. • Aptamers developed by Capture-SELEX for environmental monitoring are reviewed.

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LYU C. et al. Capture-SELEX for aptamer selection: A short review // Talanta. 2021. Vol. 229. p. 122274.

GOST all authors (up to 50) Copy

LYU C., Khan I., Wang Z. Capture-SELEX for aptamer selection: A short review // Talanta. 2021. Vol. 229. p. 122274.

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RIS Copy

TY - JOUR

DO - 10.1016/j.talanta.2021.122274

UR - https://doi.org/10.1016/j.talanta.2021.122274

TI - Capture-SELEX for aptamer selection: A short review

T2 - Talanta

AU - LYU, CHEN

AU - Khan, Imran

AU - Wang, Zhouping

PY - 2021

DA - 2021/07/01 00:00:00

PB - Elsevier

SP - 122274

VL - 229

SN - 0039-9140

SN - 1873-3573

ER -

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@article{2021_LYU,

author = {CHEN LYU and Imran Khan and Zhouping Wang},

title = {Capture-SELEX for aptamer selection: A short review},

journal = {Talanta},

year = {2021},

volume = {229},

publisher = {Elsevier},

month = {jul},

url = {https://doi.org/10.1016/j.talanta.2021.122274},

pages = {122274},

doi = {10.1016/j.talanta.2021.122274}

}

Found error?

Publisher

Elsevier

Journal

Talanta

Quartile SCImago

Quartile WOS

Impact factor

6.1

ISSN

00399140 (Print)
18733573 (Electronic)