Talanta, volume 252, pages 123838
Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker
2
REQUIMTE–LAQV, School of Engineering, Polytechnic Institute of Porto, R. Dr. António Bernardino de Almeida 431, 4200-072, Porto, Portugal
|
Publication type: Journal Article
Publication date: 2023-01-01
Analytical Chemistry
Abstract
Aggregation of α-synuclein has been recognized as a critical event in the pathogenesis of Parkinson's disease whose prevalence is increasing with great socio-economic challenges for future generations. Here, we developed a sensitive and specific electrochemical immunosensor for the detection and quantification of this biomarker, based on the voltammetric study of a redox indicator signal, which decreases upon the analyte recognition by the antibody due to the electronic resistance increase. The proposed immunosensor is based on a screen-printed carbon electrode modified in a layer-by-layer approach, which through extensive characterization led to the successful nanostructuration of the transducer, through the drop-cast of 3.0 μL of a 0.1 mg mL-1 single-walled carbon nanotubes suspension followed by electrodeposition of gold nanoparticles in a 3 mM HAuCl4 solution under a -0.2 V potential for 150 s. Monoclonal antibodies were immobilized on the gold nanoparticles surface through chemical modification at an optimal concentration of 200 μg mL-1. Using the proposed immunosensor, α-synuclein was detected in the range of 0.01-10 ng mL-1 with a 4.1 and 12.6 pg mL-1 limits of detection and quantification, respectively. Recovery values of 96.7, 106.2 and 102.9% were attained for the tested concentrations spiked in fetal bovine serum while also presenting excellent specificity and stability throughout one month. The nanostructured immunosensor provided a great interface for electronic transduction and biological recognition events, which enabled fast, sensitive and specific detection of α-synuclein while being based on a simple and inexpensive technology requiring small sample volumes, crucial characteristics for application in point-of-care testing.
Top-30
Citations by journals
1
2
|
|
TrAC - Trends in Analytical Chemistry
2 publications, 15.38%
|
|
Talanta
1 publication, 7.69%
|
|
Nanoscale Advances
1 publication, 7.69%
|
|
Sensors and Actuators, B: Chemical
1 publication, 7.69%
|
|
Diamond and Related Materials
1 publication, 7.69%
|
|
Biosensors and Bioelectronics: X
1 publication, 7.69%
|
|
Microchimica Acta
1 publication, 7.69%
|
|
Materials
1 publication, 7.69%
|
|
Russian Chemical Reviews
1 publication, 7.69%
|
|
The Analyst
1 publication, 7.69%
|
|
1
2
|
Citations by publishers
1
2
3
4
5
6
7
|
|
Elsevier
7 publications, 53.85%
|
|
Royal Society of Chemistry (RSC)
2 publications, 15.38%
|
|
Springer Nature
1 publication, 7.69%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 7.69%
|
|
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 7.69%
|
|
IEEE
1 publication, 7.69%
|
|
1
2
3
4
5
6
7
|
- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2023,2024],"ids":[0,0],"codes":[0,0],"imageUrls":["",""],"datasets":[{"label":"Citations number","data":[2,11],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":["15.38","84.62"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["TrAC - Trends in Analytical Chemistry","Talanta","Nanoscale Advances","Sensors and Actuators, B: Chemical","Diamond and Related Materials","Biosensors and Bioelectronics: X","Microchimica Acta","Materials","Russian Chemical Reviews","The Analyst"],"ids":[434,4331,16663,22419,14497,9166,11314,25198,23802,9343],"codes":[0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/9Mus3KG1Tkd7Bwaurt8H3RwWh0CxRlGoO6ng9UK1_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp"],"datasets":[{"label":"","data":[2,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[15.38,7.69,7.69,7.69,7.69,7.69,7.69,7.69,7.69,7.69],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Royal Society of Chemistry (RSC)","Springer Nature","Multidisciplinary Digital Publishing Institute (MDPI)","Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii","IEEE"],"ids":[17,123,8,202,9422,6953],"codes":[0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/9Mus3KG1Tkd7Bwaurt8H3RwWh0CxRlGoO6ng9UK1_medium.webp","\/storage\/images\/resized\/6scCJegesojp2jubwY3uKCzTAmgsaH2GIFlg6Hfk_medium.webp"],"datasets":[{"label":"","data":[7,2,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[53.85,15.38,7.69,7.69,7.69,7.69],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartiles":{"type":"bar","data":{"show":true,"labels":[2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,0],"percentage":["0","0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,0],"percentage":["0","0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[0,2],"percentage":["0","15.38"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[2,7],"percentage":["15.38","53.85"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[0,2],"percentage":["0","15.38"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by SCImago per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartilesWs":{"type":"bar","data":{"show":true,"labels":[2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,0],"percentage":["0","0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,0],"percentage":["0","0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[1,3],"percentage":["7.69","23.08"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[1,5],"percentage":["7.69","38.46"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[0,3],"percentage":["0","23.08"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by WoS per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Carneiro P. et al. Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker // Talanta. 2023. Vol. 252. p. 123838.
GOST all authors (up to 50)
Copy
Carneiro P., Loureiro J. R., Delerue-Matos C., Morais S., Gouveia Pereira M. Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker // Talanta. 2023. Vol. 252. p. 123838.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/j.talanta.2022.123838
UR - https://doi.org/10.1016/j.talanta.2022.123838
TI - Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker
T2 - Talanta
AU - Carneiro, Pedro
AU - Loureiro, Joana R.A.
AU - Delerue-Matos, Cristina
AU - Morais, Simone
AU - Gouveia Pereira, Maria
PY - 2023
DA - 2023/01/01 00:00:00
PB - Elsevier
SP - 123838
VL - 252
SN - 0039-9140
SN - 1873-3573
ER -
Cite this
BibTex
Copy
@article{2023_Carneiro,
author = {Pedro Carneiro and Joana R.A. Loureiro and Cristina Delerue-Matos and Simone Morais and Maria Gouveia Pereira},
title = {Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker},
journal = {Talanta},
year = {2023},
volume = {252},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016/j.talanta.2022.123838},
pages = {123838},
doi = {10.1016/j.talanta.2022.123838}
}
Profiles