Talanta, volume 252, pages 123838
Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker
2
REQUIMTE–LAQV, School of Engineering, Polytechnic Institute of Porto, R. Dr. António Bernardino de Almeida 431, 4200-072, Porto, Portugal
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Publication type: Journal Article
Publication date: 2023-01-01
Analytical Chemistry
Abstract
Aggregation of α-synuclein has been recognized as a critical event in the pathogenesis of Parkinson's disease whose prevalence is increasing with great socio-economic challenges for future generations. Here, we developed a sensitive and specific electrochemical immunosensor for the detection and quantification of this biomarker, based on the voltammetric study of a redox indicator signal, which decreases upon the analyte recognition by the antibody due to the electronic resistance increase. The proposed immunosensor is based on a screen-printed carbon electrode modified in a layer-by-layer approach, which through extensive characterization led to the successful nanostructuration of the transducer, through the drop-cast of 3.0 μL of a 0.1 mg mL-1 single-walled carbon nanotubes suspension followed by electrodeposition of gold nanoparticles in a 3 mM HAuCl4 solution under a -0.2 V potential for 150 s. Monoclonal antibodies were immobilized on the gold nanoparticles surface through chemical modification at an optimal concentration of 200 μg mL-1. Using the proposed immunosensor, α-synuclein was detected in the range of 0.01-10 ng mL-1 with a 4.1 and 12.6 pg mL-1 limits of detection and quantification, respectively. Recovery values of 96.7, 106.2 and 102.9% were attained for the tested concentrations spiked in fetal bovine serum while also presenting excellent specificity and stability throughout one month. The nanostructured immunosensor provided a great interface for electronic transduction and biological recognition events, which enabled fast, sensitive and specific detection of α-synuclein while being based on a simple and inexpensive technology requiring small sample volumes, crucial characteristics for application in point-of-care testing.
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IEEE
1 publication, 7.69%
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- We do not take into account publications without a DOI.
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Carneiro P. et al. Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker // Talanta. 2023. Vol. 252. p. 123838.
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Carneiro P., Loureiro J. R., Delerue-Matos C., Morais S., Gouveia Pereira M. Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker // Talanta. 2023. Vol. 252. p. 123838.
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TY - JOUR
DO - 10.1016/j.talanta.2022.123838
UR - https://doi.org/10.1016/j.talanta.2022.123838
TI - Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker
T2 - Talanta
AU - Carneiro, Pedro
AU - Loureiro, Joana R.A.
AU - Delerue-Matos, Cristina
AU - Morais, Simone
AU - Gouveia Pereira, Maria
PY - 2023
DA - 2023/01/01 00:00:00
PB - Elsevier
SP - 123838
VL - 252
SN - 0039-9140
SN - 1873-3573
ER -
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@article{2023_Carneiro,
author = {Pedro Carneiro and Joana R.A. Loureiro and Cristina Delerue-Matos and Simone Morais and Maria Gouveia Pereira},
title = {Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker},
journal = {Talanta},
year = {2023},
volume = {252},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016/j.talanta.2022.123838},
pages = {123838},
doi = {10.1016/j.talanta.2022.123838}
}
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