Talanta, volume 264, pages 124773

Detection and imaging of bacterial biofilms with glutathione-stabilized gold nanoclusters

Evstigneeva Stella S ¹

Chumakov D. S. ¹

Tumskiy Roman S ¹

Khlebtsov Boris ^{1, 2}

Khlebtsov Nikolai ^{1, 2}

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Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 410049 13 Prospekt Entuziastov, Saratov, 410049, Russia

Institute of Biochemistry and Physiology of Plants and Microorganisms SarSc of the Russian Academy of Sciences

Saratov Scientific Center of the Russian Academy of Sciences

Institute of Physics, Saratov State University, 410012, Saratov, Russia |

Publication type: Journal Article

Publication date: 2023-11-01

Elsevier

Journal: Talanta

Quartile SCImago

Quartile WOS

Impact factor: 6.1

ISSN: 00399140, 18733573

DOI: 10.1016/j.talanta.2023.124773

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Analytical Chemistry

Abstract

Bacterial biofilms colonize chronic wounds and surfaces of medical devices, thus making the development of reliable methods for imaging and detection of biofilms crucial. Although fluorescent identification of bacteria is sensitive and non-destructive, the lack of biofilm-specific fluorescent dyes limits the application of this technique to biofilm detection. Here, we demonstrate, for the first time, that fluorescent glutathione-stabilized gold nanoclusters (GSH-AuNCs) without targeting ligands can specifically interact with extracellular matrix components of Gram-negative and Gram-positive bacterial biofilms resulting in fluorescent staining of bacterial biofilms. By contrast, fluorescent bovine serum albumin-stabilized gold nanoclusters and 11-mercaptoundecanoic acid - stabilized gold nanoclusters do not stain the extracellular matrix of biofilms. According to molecular docking studies, GSH-AuNCs show affinity to several targets in extracellular matrix, including amyloid-anchoring proteins, matrix proteins and polysaccharides. Some experimental evidence was obtained for the interaction of GSH-AuNCs with the lipopolysaccharide (LPS) that was isolated from the matrix of Azospirillum baldaniorum biofilms. Based on GSH-AuNCs properties, we propose a new fluorescent method for the measurement of biofilm biomass with a limit of detection 1.7 × 105 CFU/mL. The sensitivity of the method is 10-fold higher than the standard biofilm quantification with the crystal violet assay. There is a good linear relationship between the fluorescence intensity from the biofilms and the number of CFU from the biofilms in the range from 2.6 × 105 to 6.7 × 107 CFU/mL. The developed nanocluster-mediated method of biofilm staining was successfully applied for quantitative detection of biofilm formation on urinary catheter surface. The presented data suggest that fluorescent GSH-AuNCs can be used to diagnose medical device-associated infections.

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Citations by journals

	1
ACS applied materials & interfaces	ACS applied materials & interfaces, 1, 14.29% ACS applied materials & interfaces 1 publication, 14.29%
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 1, 14.29% Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy 1 publication, 14.29%
Progress in Materials Science	Progress in Materials Science, 1, 14.29% Progress in Materials Science 1 publication, 14.29%
Science of the Total Environment	Science of the Total Environment, 1, 14.29% Science of the Total Environment 1 publication, 14.29%
Advanced Materials	Advanced Materials, 1, 14.29% Advanced Materials 1 publication, 14.29%
Microchemical Journal	Microchemical Journal, 1, 14.29% Microchemical Journal 1 publication, 14.29%
Chinese Chemical Letters	Chinese Chemical Letters, 1, 14.29% Chinese Chemical Letters 1 publication, 14.29%
	1

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	1 2 3 4 5
Elsevier	Elsevier, 5, 71.43% Elsevier 5 publications, 71.43%
American Chemical Society (ACS)	American Chemical Society (ACS), 1, 14.29% American Chemical Society (ACS) 1 publication, 14.29%
Wiley	Wiley, 1, 14.29% Wiley 1 publication, 14.29%
	1 2 3 4 5

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Evstigneeva S. S. et al. Detection and imaging of bacterial biofilms with glutathione-stabilized gold nanoclusters // Talanta. 2023. Vol. 264. p. 124773.

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Evstigneeva S. S., Chumakov D. S., Tumskiy R. S., Khlebtsov B., Khlebtsov N. Detection and imaging of bacterial biofilms with glutathione-stabilized gold nanoclusters // Talanta. 2023. Vol. 264. p. 124773.

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RIS Copy

TY - JOUR

DO - 10.1016/j.talanta.2023.124773

UR - https://doi.org/10.1016%2Fj.talanta.2023.124773

TI - Detection and imaging of bacterial biofilms with glutathione-stabilized gold nanoclusters

T2 - Talanta

AU - Evstigneeva, Stella S

AU - Chumakov, D. S.

AU - Tumskiy, Roman S

AU - Khlebtsov, Boris

AU - Khlebtsov, Nikolai

PY - 2023

DA - 2023/11/01 00:00:00

PB - Elsevier

SP - 124773

VL - 264

SN - 0039-9140

SN - 1873-3573

ER -

BibTex

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BibTex Copy

@article{2023_Evstigneeva,

author = {Stella S Evstigneeva and D. S. Chumakov and Roman S Tumskiy and Boris Khlebtsov and Nikolai Khlebtsov},

title = {Detection and imaging of bacterial biofilms with glutathione-stabilized gold nanoclusters},

journal = {Talanta},

year = {2023},

volume = {264},

publisher = {Elsevier},

month = {nov},

url = {https://doi.org/10.1016%2Fj.talanta.2023.124773},

pages = {124773},

doi = {10.1016/j.talanta.2023.124773}

}

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Publisher

Elsevier

Journal

Talanta

Quartile SCImago

Quartile WOS

Impact factor

6.1

ISSN

00399140 (Print)
18733573 (Electronic)

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