ACS Biomaterials Science and Engineering, volume 6, issue 10, pages 5744-5757
In Vitro and in Vivo Analysis of Adhesive, Anti-Inflammatory, and Proangiogenic Properties of Novel 3D Printed Hyaluronic Acid Glycidyl Methacrylate Hydrogel Scaffolds for Tissue Engineering
Später Thomas
1
,
Mariyanats Aleksandra O
2
,
Syachina Maria A
2
,
Mironov Anton V
2
,
Savelyev A.G.
2, 3
,
Sochilina Anastasia
2, 4
,
Menger Michael D.
1
,
Kananykhina Evgeniya Y
6
,
Sukhikh Gennady
5
,
Spitkovsky Dmitry D
5
,
Katsen-Globa Alisa
1
,
Laschke Matthias W.
1
,
1
Institute for Clinical & Experimental Surgery, Saarland University, 66421 Homburg/Saar, Germany
|
2
Publication type: Journal Article
Publication date: 2020-08-19
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 5.8
ISSN: 23739878
Biomaterials
Biomedical Engineering
Abstract
In this study, we prepared hydrogel scaffolds for tissue engineering by computer-assisted extrusion three-dimensional (3D) printing with photocured (λ = 445 nm) hyaluronic acid glycidyl methacrylate (HAGM). The developed product was compared with the polylactic-co-glycolic acid (PLGA) scaffolds generated by means of the original antisolvent 3D printing methodology. The cytotoxicity and cytocompatibility of the scaffolds were analyzed in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests, flow cytometry, and scanning electron microscopy. Anti-inflammatory and proangiogenic properties of the scaffolds were evaluated in the dorsal skinfold chamber mouse model by means of intravital fluorescence microscopy, histology, and immunohistochemistry throughout an observation period of 14 days. In vitro, none of the scaffolds revealed cytotoxicity on days 1, 2, and 5 after seeding with umbilical cord-derived multipotent stromal cells, and the primary cell adhesion to the surface of HAGM scaffolds was low. In vivo, implanted HAGM scaffolds showed enhanced vascularization and host tissue ingrowth, and the inflammatory response to them was less pronounced compared with PLGA scaffolds. The results indicate excellent biocompatibility and vascularization capacity of the developed 3D printed HAGM scaffolds and position them as strong candidates for advanced tissue engineering applications.
Citations by journals
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
2 publications, 8.7%
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ACS Applied Bio Materials
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2 publications, 8.7%
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International Materials Reviews
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International Materials Reviews
1 publication, 4.35%
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1 publication, 4.35%
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Frontiers in Bioengineering and Biotechnology
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Frontiers in Bioengineering and Biotechnology
1 publication, 4.35%
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Polymers
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1 publication, 4.35%
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Molecules
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Molecules
1 publication, 4.35%
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Life
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Life
1 publication, 4.35%
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International Journal of Biological Macromolecules
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International Journal of Biological Macromolecules
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Bioprinting
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1 publication, 4.35%
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Gels
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Gels
1 publication, 4.35%
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Smart Materials in Medicine
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1 publication, 4.35%
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Biomedical Materials (Bristol)
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Biomedical Materials (Bristol)
1 publication, 4.35%
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Journal of Drug Delivery Science and Technology
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Journal of Drug Delivery Science and Technology
1 publication, 4.35%
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Materials Horizons
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Materials Horizons
1 publication, 4.35%
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Journal of Biomaterials Science, Polymer Edition
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Journal of Biomaterials Science, Polymer Edition
1 publication, 4.35%
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Journal of Dental Sciences
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Journal of Dental Sciences, 1, 4.35%
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1 publication, 4.35%
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Colloids and Surfaces B: Biointerfaces
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Colloids and Surfaces B: Biointerfaces
1 publication, 4.35%
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Tissue Engineering - Part B: Reviews
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1 publication, 4.35%
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Expert Opinion on Drug Delivery
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Expert Opinion on Drug Delivery
1 publication, 4.35%
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Citations by publishers
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7
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
7 publications, 30.43%
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Elsevier
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Elsevier
7 publications, 30.43%
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Taylor & Francis
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Taylor & Francis
3 publications, 13.04%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
2 publications, 8.7%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 4.35%
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IOP Publishing
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IOP Publishing
1 publication, 4.35%
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Royal Society of Chemistry (RSC)
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Royal Society of Chemistry (RSC)
1 publication, 4.35%
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Mary Ann Liebert
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Mary Ann Liebert
1 publication, 4.35%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Später T. et al. In Vitro and in Vivo Analysis of Adhesive, Anti-Inflammatory, and Proangiogenic Properties of Novel 3D Printed Hyaluronic Acid Glycidyl Methacrylate Hydrogel Scaffolds for Tissue Engineering // ACS Biomaterials Science and Engineering. 2020. Vol. 6. No. 10. pp. 5744-5757.
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Später T., Mariyanats A. O., Syachina M. A., Mironov A. V., Savelyev A., Sochilina A., Menger M. D., Vishnyakova P., Kananykhina E. Y., Fatkhudinov T., Sukhikh G., Spitkovsky D. D., Katsen-Globa A., Laschke M. W., Popov V. K. In Vitro and in Vivo Analysis of Adhesive, Anti-Inflammatory, and Proangiogenic Properties of Novel 3D Printed Hyaluronic Acid Glycidyl Methacrylate Hydrogel Scaffolds for Tissue Engineering // ACS Biomaterials Science and Engineering. 2020. Vol. 6. No. 10. pp. 5744-5757.
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TY - JOUR
DO - 10.1021/acsbiomaterials.0c00741
UR - https://doi.org/10.1021%2Facsbiomaterials.0c00741
TI - In Vitro and in Vivo Analysis of Adhesive, Anti-Inflammatory, and Proangiogenic Properties of Novel 3D Printed Hyaluronic Acid Glycidyl Methacrylate Hydrogel Scaffolds for Tissue Engineering
T2 - ACS Biomaterials Science and Engineering
AU - Syachina, Maria A
AU - Mironov, Anton V
AU - Kananykhina, Evgeniya Y
AU - Spitkovsky, Dmitry D
AU - Katsen-Globa, Alisa
AU - Mariyanats, Aleksandra O
AU - Savelyev, A.G.
AU - Popov, Vladimir K
AU - Später, Thomas
AU - Sochilina, Anastasia
AU - Menger, Michael D.
AU - Vishnyakova, Polina
AU - Fatkhudinov, Timur
AU - Sukhikh, Gennady
AU - Laschke, Matthias W.
PY - 2020
DA - 2020/08/19 00:00:00
PB - American Chemical Society (ACS)
SP - 5744-5757
IS - 10
VL - 6
SN - 2373-9878
ER -
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@article{2020_Später,
author = {Maria A Syachina and Anton V Mironov and Evgeniya Y Kananykhina and Dmitry D Spitkovsky and Alisa Katsen-Globa and Aleksandra O Mariyanats and A.G. Savelyev and Vladimir K Popov and Thomas Später and Anastasia Sochilina and Michael D. Menger and Polina Vishnyakova and Timur Fatkhudinov and Gennady Sukhikh and Matthias W. Laschke},
title = {In Vitro and in Vivo Analysis of Adhesive, Anti-Inflammatory, and Proangiogenic Properties of Novel 3D Printed Hyaluronic Acid Glycidyl Methacrylate Hydrogel Scaffolds for Tissue Engineering},
journal = {ACS Biomaterials Science and Engineering},
year = {2020},
volume = {6},
publisher = {American Chemical Society (ACS)},
month = {aug},
url = {https://doi.org/10.1021%2Facsbiomaterials.0c00741},
number = {10},
pages = {5744--5757},
doi = {10.1021/acsbiomaterials.0c00741}
}
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Später, Thomas, et al. “In Vitro and in Vivo Analysis of Adhesive, Anti-Inflammatory, and Proangiogenic Properties of Novel 3D Printed Hyaluronic Acid Glycidyl Methacrylate Hydrogel Scaffolds for Tissue Engineering.” ACS Biomaterials Science and Engineering, vol. 6, no. 10, Aug. 2020, pp. 5744-5757. https://doi.org/10.1021%2Facsbiomaterials.0c00741.
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