Open Access
ACS Omega, volume 5, issue 12, pages 6641-6650
Synthesis of Inorganic Pyrophosphatase-Nanodiamond Conjugates Resistant to Calcium and Fluoride
Valueva Anastasiya V.
1
,
Roman Lucimara S
2
,
Vorobyeva Nataliya N.
3
,
Kurilova Svetlana A
4
,
Rodina Elena V.
3
Publication type: Journal Article
Publication date: 2020-03-20
PubMed ID:
32258899
General Chemistry
General Chemical Engineering
Abstract
Pyrophosphate arthropathy is the mineralization defect in humans caused by the deposition of microcrystals of calcium pyrophosphate dihydrate in joint tissues. As a potential therapeutic strategy for the treatment of pyrophosphate arthropathy, delivery of exogenous pyrophosphate-hydrolyzing enzymes, inorganic pyrophosphatases (PPases), to the synovial fluid has been suggested. Previously, we synthesized the conjugates of Escherichia coli PPase (Ec-PPase) with detonation synthesis nanodiamonds (NDs) as a delivery platform, obtaining the hybrid biomaterial retaining high pyrophosphate-hydrolyzing activity in vitro. However, most known PPases including Ec-PPase in the soluble form are strongly inhibited by Ca2+ ions. Because synovial fluid contains up to millimolar concentrations of soluble calcium, this inhibition might limit the in vivo application of Ec-PPase-based material in joint tissues. In this work, we proposed other bacterial PPases from Mycobacterium tuberculosis (Mt-PPase), which are resistant to the inhibition by Ca2+ ions, as an active PPi-hydrolyzing agent. We synthesized conjugates of Mt-PPase with NDs and tested their activity under various conditions. Unexpectedly, conjugates of both Ec-PPase and Mt-PPase with aminated NDs retained significant hydrolytic activity in the presence of well-known mechanism-based PPase inhibitors, fluoride or calcium. The incomplete inhibition of PPases by fluoride or calcium was found for the first time.
Citations by journals
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Biomolecular NMR Assignments
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Biomolecular NMR Assignments
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
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1
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Springer Nature
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Springer Nature
1 publication, 50%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 50%
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1
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Valueva A. V. et al. Synthesis of Inorganic Pyrophosphatase-Nanodiamond Conjugates Resistant to Calcium and Fluoride // ACS Omega. 2020. Vol. 5. No. 12. pp. 6641-6650.
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Valueva A. V., Roman L. S., Vorobyeva N. N., Kurilova S. A., Rodina E. V. Synthesis of Inorganic Pyrophosphatase-Nanodiamond Conjugates Resistant to Calcium and Fluoride // ACS Omega. 2020. Vol. 5. No. 12. pp. 6641-6650.
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TY - JOUR
DO - 10.1021/ACSOMEGA.9B04428
UR - https://doi.org/10.1021%2FACSOMEGA.9B04428
TI - Synthesis of Inorganic Pyrophosphatase-Nanodiamond Conjugates Resistant to Calcium and Fluoride
T2 - ACS Omega
AU - Valueva, Anastasiya V.
AU - Vorobyeva, Nataliya N.
AU - Kurilova, Svetlana A
AU - Rodina, Elena V.
AU - Roman, Lucimara S
PY - 2020
DA - 2020/03/20 00:00:00
PB - American Chemical Society (ACS)
SP - 6641-6650
IS - 12
VL - 5
PMID - 32258899
SN - 2470-1343
ER -
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@article{2020_Valueva,
author = {Anastasiya V. Valueva and Nataliya N. Vorobyeva and Svetlana A Kurilova and Elena V. Rodina and Lucimara S Roman},
title = {Synthesis of Inorganic Pyrophosphatase-Nanodiamond Conjugates Resistant to Calcium and Fluoride},
journal = {ACS Omega},
year = {2020},
volume = {5},
publisher = {American Chemical Society (ACS)},
month = {mar},
url = {https://doi.org/10.1021%2FACSOMEGA.9B04428},
number = {12},
pages = {6641--6650},
doi = {10.1021/ACSOMEGA.9B04428}
}
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Valueva, Anastasiya V., et al. “Synthesis of Inorganic Pyrophosphatase-Nanodiamond Conjugates Resistant to Calcium and Fluoride.” ACS Omega, vol. 5, no. 12, Mar. 2020, pp. 6641-6650. https://doi.org/10.1021%2FACSOMEGA.9B04428.
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