Open Access
ACS Omega, volume 5, issue 15, pages 8579-8586
Inorganic Pyrophosphatase-Nanodiamond Conjugates Hydrolyze Pyrophosphate in Human Synovial Fluid
Publication type: Journal Article
Publication date: 2020-04-07
PubMed ID:
32337420
General Chemistry
General Chemical Engineering
Abstract
The present work is focused on testing enzyme-based agents for the partial dissolution of calcium pyrophosphate (CaPPi) deposits in the cartilages and synovial fluid of patients with pyrophosphate arthropathy (CPPD disease). Previously, we suggested that inorganic pyrophosphatases (PPases) immobilized on nanodiamonds of detonation synthesis (NDs) could be appropriate for this purpose. We synthesized and characterized conjugates of NDs and PPases from Escherichia coli and Mycobacterium tuberculosis. The conjugates showed high enzymatic activity and resistance to inhibition by calcium and fluoride. Here, we tested the effectiveness of pyrophosphate (PPi) hydrolysis by the conjugates in an in vitro model system simulating the ionic composition of the synovial fluid and in the samples of synovial fluid of patients with CPPD via NMR spectroscopy. The conjugates of both PPases efficiently hydrolyzed triclinic crystalline calcium pyrophosphate (t-CPPD) in the model system. We evaluated the number of phosphorus-containing compounds in the synovial fluid, showed the possibility of PPi detection in it, and estimated the hydrolytic activity of the PPase conjugates. The soluble and immobilized PPases were able to hydrolyze a significant amount of PPi (1 mM) in the synovial fluid in short periods of time (24 h). The maximum activity was demonstrated for Mt-PPase immobilized on ND–NH–(CH2)6–NH2 (2.24 U mg–1).
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Biomolecular NMR Assignments
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Biomolecular NMR Assignments
1 publication, 25%
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Journal of the Electrochemical Society
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Journal of the Electrochemical Society
1 publication, 25%
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
1 publication, 25%
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Biosensors and Bioelectronics: X
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Biosensors and Bioelectronics: X
1 publication, 25%
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Springer Nature
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Springer Nature
1 publication, 25%
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The Electrochemical Society
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The Electrochemical Society
1 publication, 25%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 25%
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Elsevier
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Elsevier
1 publication, 25%
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Valueva A. V. et al. Inorganic Pyrophosphatase-Nanodiamond Conjugates Hydrolyze Pyrophosphate in Human Synovial Fluid // ACS Omega. 2020. Vol. 5. No. 15. pp. 8579-8586.
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Valueva A. V., Roman L. S., Mariasina S. S., Eliseev M. S., Rodina E. V. Inorganic Pyrophosphatase-Nanodiamond Conjugates Hydrolyze Pyrophosphate in Human Synovial Fluid // ACS Omega. 2020. Vol. 5. No. 15. pp. 8579-8586.
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TY - JOUR
DO - 10.1021/ACSOMEGA.9B04429
UR - https://doi.org/10.1021%2FACSOMEGA.9B04429
TI - Inorganic Pyrophosphatase-Nanodiamond Conjugates Hydrolyze Pyrophosphate in Human Synovial Fluid
T2 - ACS Omega
AU - Valueva, Anastasiya V.
AU - Mariasina, Sofia S
AU - Eliseev, Maxim S
AU - Rodina, Elena V.
AU - Roman, Lucimara S
PY - 2020
DA - 2020/04/07 00:00:00
PB - American Chemical Society (ACS)
SP - 8579-8586
IS - 15
VL - 5
PMID - 32337420
SN - 2470-1343
ER -
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@article{2020_Valueva,
author = {Anastasiya V. Valueva and Sofia S Mariasina and Maxim S Eliseev and Elena V. Rodina and Lucimara S Roman},
title = {Inorganic Pyrophosphatase-Nanodiamond Conjugates Hydrolyze Pyrophosphate in Human Synovial Fluid},
journal = {ACS Omega},
year = {2020},
volume = {5},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1021%2FACSOMEGA.9B04429},
number = {15},
pages = {8579--8586},
doi = {10.1021/ACSOMEGA.9B04429}
}
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Valueva, Anastasiya V., et al. “Inorganic Pyrophosphatase-Nanodiamond Conjugates Hydrolyze Pyrophosphate in Human Synovial Fluid.” ACS Omega, vol. 5, no. 15, Apr. 2020, pp. 8579-8586. https://doi.org/10.1021%2FACSOMEGA.9B04429.
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