Differential Pulsed Voltammetric Determination of RNA at the Picomole Level in the Presence of DNA and Nucleic Acid Components
Publication type: Journal Article
Publication date: 1994-04-01
scimago Q1
wos Q1
SJR: 1.533
CiteScore: 11.6
Impact factor: 6.7
ISSN: 00032700, 15206882, 21542686
Analytical Chemistry
Abstract
A procedure for determination of nanogram quantities of RNA in the presence of an excess of DNA and monomeric nucleic acid constituents is presented. The procedure is based on immobilization of RNA by incubation of a hanging mercury drop electrode in a 5-μL sample followed by washing and transfer of the nucleic acid-modified electrode in a voltammetric cell containing blank background electrolyte. The differential pulse voltammetric response of RNA differs from that of DNA. This makes it possible to determine traces (less than 1%) of RNA in DNA samples. Low molecular mass substances such as bases, nucleosides, and nucleotides do not interfere with the RNA determination as they do not adsorb at the electrode surface or are removed during the washing step. The limit of detection is below 100 pg of RNA
Found
Nothing found, try to update filter.
Top-30
Journals
|
5
10
15
20
|
|
|
Electroanalysis
20 publications, 26.32%
|
|
|
Analytical Chemistry
13 publications, 17.11%
|
|
|
Bioelectrochemistry and Bioenergetics
4 publications, 5.26%
|
|
|
Analytica Chimica Acta
4 publications, 5.26%
|
|
|
Journal of Electroanalytical Chemistry
4 publications, 5.26%
|
|
|
Biosensors and Bioelectronics
3 publications, 3.95%
|
|
|
The Analyst
3 publications, 3.95%
|
|
|
Electrochimica Acta
2 publications, 2.63%
|
|
|
Collection of Czechoslovak Chemical Communications
2 publications, 2.63%
|
|
|
Langmuir
1 publication, 1.32%
|
|
|
Journal of Physical Chemistry B
1 publication, 1.32%
|
|
|
Inorganic Chemistry
1 publication, 1.32%
|
|
|
Chemico-Biological Interactions
1 publication, 1.32%
|
|
|
Analytical Biochemistry
1 publication, 1.32%
|
|
|
Bioelectrochemistry
1 publication, 1.32%
|
|
|
Journal of Pharmaceutical and Biomedical Analysis
1 publication, 1.32%
|
|
|
Journal of the Serbian Chemical Society
1 publication, 1.32%
|
|
|
Analytical and Bioanalytical Chemistry
1 publication, 1.32%
|
|
|
Microchimica Acta
1 publication, 1.32%
|
|
|
Bioanalytical Reviews
1 publication, 1.32%
|
|
|
Monatshefte fur Chemie
1 publication, 1.32%
|
|
|
Electrochemistry Communications
1 publication, 1.32%
|
|
|
Analytical Letters
1 publication, 1.32%
|
|
|
Advances in Inorganic Chemistry
1 publication, 1.32%
|
|
|
Perspectives in Bioanalysis
1 publication, 1.32%
|
|
|
Reviews in Analytical Chemistry
1 publication, 1.32%
|
|
|
5
10
15
20
|
Publishers
|
5
10
15
20
25
|
|
|
Elsevier
24 publications, 31.58%
|
|
|
Wiley
20 publications, 26.32%
|
|
|
American Chemical Society (ACS)
16 publications, 21.05%
|
|
|
Springer Nature
4 publications, 5.26%
|
|
|
Royal Society of Chemistry (RSC)
3 publications, 3.95%
|
|
|
Institute of Organic Chemistry & Biochemistry
2 publications, 2.63%
|
|
|
National Library of Serbia
1 publication, 1.32%
|
|
|
Taylor & Francis
1 publication, 1.32%
|
|
|
Walter de Gruyter
1 publication, 1.32%
|
|
|
5
10
15
20
25
|
- We do not take into account publications without a DOI.
- Statistics recalculated weekly.
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
76
Total citations:
76
Citations from 2024:
0
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Palecek E., Fojta M. Differential Pulsed Voltammetric Determination of RNA at the Picomole Level in the Presence of DNA and Nucleic Acid Components // Analytical Chemistry. 1994. Vol. 66. No. 9. pp. 1566-1571.
GOST all authors (up to 50)
Copy
Palecek E., Fojta M. Differential Pulsed Voltammetric Determination of RNA at the Picomole Level in the Presence of DNA and Nucleic Acid Components // Analytical Chemistry. 1994. Vol. 66. No. 9. pp. 1566-1571.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1021/ac00081a033
UR - https://doi.org/10.1021/ac00081a033
TI - Differential Pulsed Voltammetric Determination of RNA at the Picomole Level in the Presence of DNA and Nucleic Acid Components
T2 - Analytical Chemistry
AU - Palecek, Emil
AU - Fojta, Miroslav
PY - 1994
DA - 1994/04/01
PB - American Chemical Society (ACS)
SP - 1566-1571
IS - 9
VL - 66
SN - 0003-2700
SN - 1520-6882
SN - 2154-2686
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{1994_Palecek,
author = {Emil Palecek and Miroslav Fojta},
title = {Differential Pulsed Voltammetric Determination of RNA at the Picomole Level in the Presence of DNA and Nucleic Acid Components},
journal = {Analytical Chemistry},
year = {1994},
volume = {66},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1021/ac00081a033},
number = {9},
pages = {1566--1571},
doi = {10.1021/ac00081a033}
}
Cite this
MLA
Copy
Palecek, Emil, and Miroslav Fojta. “Differential Pulsed Voltammetric Determination of RNA at the Picomole Level in the Presence of DNA and Nucleic Acid Components.” Analytical Chemistry, vol. 66, no. 9, Apr. 1994, pp. 1566-1571. https://doi.org/10.1021/ac00081a033.