Accounts of Chemical Research, volume 55, issue 4, pages 551-564
Multiplexed Biosensing and Bioimaging Using Lanthanide-Based Time-Gated Förster Resonance Energy Transfer
2
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
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3
nanofret.com, Laboratoire COBRA, Université de Rouen Normandie, Normandie Université, CNRS, INSA Rouen, 76000 Rouen, France
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4
Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, 91198 Gif-sur-Yvette, France
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6
Université Paris-Saclay, 91405 Orsay Cedex, France
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Publication type: Journal Article
Publication date: 2022-01-27
Journal:
Accounts of Chemical Research
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 18.3
ISSN: 00014842, 15204898
General Chemistry
General Medicine
Abstract
The necessity to scrutinize more and more biological molecules and interactions both in solution and on the cellular level has led to an increasing demand for sensitive and specific multiplexed diagnostic analysis. Photoluminescence (PL) detection is ideally suited for multiplexed biosensing and bioimaging because it is rapid and sensitive and there is an almost unlimited choice of fluorophores that provide a large versatility of photophysical properties, including PL intensities, spectra, and lifetimes.The most frequently used technique to detect multiple parameters from a single sample is spectral (or color) multiplexing with different fluorophores, such as organic dyes, fluorescent proteins, quantum dots, or lanthanide nanoparticles and complexes. In conventional PL biosensing approaches, each fluorophore requires a distinct detection channel and excitation wavelength. This drawback can be overcome by Förster resonance energy transfer (FRET) from lanthanide donors to other fluorophore acceptors. The lanthanides' multiple and spectrally narrow emission bands over a broad spectral range can overlap with several different acceptors at once, thereby allowing FRET from one donor to multiple acceptors. The lanthanides' extremely long PL lifetimes provide two important features. First, time-gated (TG) detection allows for efficient suppression of background fluorescence from the biological environment or directly excited acceptors. Second, temporal multiplexing, for which the PL lifetimes are adjusted by the interaction with the FRET acceptor, can be used to determine specific biomolecules and/or their conformation via distinct PL decays. The high signal-to-background ratios, reproducible and precise ratiometric and homogeneous (washing-free) sensing formats, and higher-order multiplexing capabilities of lanthanide-based TG-FRET have resulted in significant advances in the analysis of biomolecular recognition. Applications range from fundamental analysis of biomolecular interactions and conformations to high-throughput and point-of-care in vitro diagnostics and DNA sequencing to advanced optical encoding, using both liquid and solid samples and in situ, in vitro, and in vivo detection with high sensitivity and selectivity.In this Account, we discuss recent advances in lanthanide-based TG-FRET for the development and application of advanced immunoassays, nucleic acid sensing, and fluorescence imaging. In addition to the different spectral and temporal multiplexing approaches, we highlight the importance of the careful design and combination of different biological, organic, and inorganic molecules and nanomaterials for an adjustable FRET donor-acceptor distance that determines the ultimate performance of the diagnostic assays and conformational sensors in their physiological environment. We conclude by sharing our vision on how progress in the development of new sensing concepts, material combinations, and instrumentation can further advance TG-FRET multiplexing and accelerate its translation into routine clinical practice and the investigation of challenging biological systems.
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Qiu X. et al. Multiplexed Biosensing and Bioimaging Using Lanthanide-Based Time-Gated Förster Resonance Energy Transfer // Accounts of Chemical Research. 2022. Vol. 55. No. 4. pp. 551-564.
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Qiu X., Xu J., Cardoso Dos Santos M., Hildebrandt N. Multiplexed Biosensing and Bioimaging Using Lanthanide-Based Time-Gated Förster Resonance Energy Transfer // Accounts of Chemical Research. 2022. Vol. 55. No. 4. pp. 551-564.
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TY - JOUR
DO - 10.1021/acs.accounts.1c00691
UR - https://doi.org/10.1021/acs.accounts.1c00691
TI - Multiplexed Biosensing and Bioimaging Using Lanthanide-Based Time-Gated Förster Resonance Energy Transfer
T2 - Accounts of Chemical Research
AU - Xu, Jingyue
AU - Hildebrandt, Niko
AU - Qiu, Xue
AU - Cardoso Dos Santos, Marcelina
PY - 2022
DA - 2022/01/27 00:00:00
PB - American Chemical Society (ACS)
SP - 551-564
IS - 4
VL - 55
SN - 0001-4842
SN - 1520-4898
ER -
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@article{2022_Qiu,
author = {Jingyue Xu and Niko Hildebrandt and Xue Qiu and Marcelina Cardoso Dos Santos},
title = {Multiplexed Biosensing and Bioimaging Using Lanthanide-Based Time-Gated Förster Resonance Energy Transfer},
journal = {Accounts of Chemical Research},
year = {2022},
volume = {55},
publisher = {American Chemical Society (ACS)},
month = {jan},
url = {https://doi.org/10.1021/acs.accounts.1c00691},
number = {4},
pages = {551--564},
doi = {10.1021/acs.accounts.1c00691}
}
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Qiu, Xue, et al. “Multiplexed Biosensing and Bioimaging Using Lanthanide-Based Time-Gated Förster Resonance Energy Transfer.” Accounts of Chemical Research, vol. 55, no. 4, Jan. 2022, pp. 551-564. https://doi.org/10.1021/acs.accounts.1c00691.