ACS Applied Bio Materials, volume 3, issue 8, pages 5120-5125
Simultaneous Determination of l -Phenylalanine, Phenylethylamine, and Phenylacetic Acid Using Three-Color Whole-Cell Biosensors within a Microchannel Device
Publication type: Journal Article
Publication date: 2020-07-15
Journal:
ACS Applied Bio Materials
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 4.6
ISSN: 25766422, 25766422
General Chemistry
Biomaterials
Biomedical Engineering
Biochemistry (medical)
Abstract
The neurotransmitter phenylethylamine (PEA) is highly susceptible to oxidation to produce phenylacetic acid (PA). The fact that PEA and PA are both metabolites of phenylalanine (Phe) in humans makes them important indicators in the diagnosis of phenylketonuria. In this work, three-color whole-cell biosensors were developed to simultaneously detect these analytes (Phe, PEA, and PA). The tyrosine-responsive promoter was used to control the production of green fluorescent protein signals in response to Phe levels. The FeaR regulon was first used to indicate the presence of PEA, whereas the Paa regulon was used for the detection of PA. The combination of three sensor strains together made it possible to semiquantify the three analytes according to unique color outputs without cross-interference. We sought to optimize various modular components (ribosomal binding sites and fluorescent proteins) to ensure the rapid generation of fluorescent signals. Finally, the biosensors were implemented within a microchannel device to reduce sample consumption in point-of-care assays.
Top-30
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1 publication, 7.69%
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1 publication, 7.69%
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1 publication, 7.69%
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1 publication, 7.69%
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1 publication, 7.69%
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Citations by publishers
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6
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Elsevier
6 publications, 46.15%
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2 publications, 15.38%
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 7.69%
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IOP Publishing
1 publication, 7.69%
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Royal Society of Chemistry (RSC)
1 publication, 7.69%
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Springer Nature
1 publication, 7.69%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 7.69%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Hsu L. et al. Simultaneous Determination of l-Phenylalanine, Phenylethylamine, and Phenylacetic Acid Using Three-Color Whole-Cell Biosensors within a Microchannel Device // ACS Applied Bio Materials. 2020. Vol. 3. No. 8. pp. 5120-5125.
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Hsu L., Lin Y., Guo J., Chen C. F., Chou Y., Yeh Y. C. Simultaneous Determination of l-Phenylalanine, Phenylethylamine, and Phenylacetic Acid Using Three-Color Whole-Cell Biosensors within a Microchannel Device // ACS Applied Bio Materials. 2020. Vol. 3. No. 8. pp. 5120-5125.
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TY - JOUR
DO - 10.1021/acsabm.0c00590
UR - https://doi.org/10.1021/acsabm.0c00590
TI - Simultaneous Determination of l-Phenylalanine, Phenylethylamine, and Phenylacetic Acid Using Three-Color Whole-Cell Biosensors within a Microchannel Device
T2 - ACS Applied Bio Materials
AU - Hsu, Li-Wen
AU - Lin, Yu-Hsuan
AU - Guo, Jun-Yu
AU - Chou, Yi-Ju
AU - Chen, Chien Fu
AU - Yeh, Yi Chun
PY - 2020
DA - 2020/07/15 00:00:00
PB - American Chemical Society (ACS)
SP - 5120-5125
IS - 8
VL - 3
SN - 2576-6422
SN - 2576-6422
ER -
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@article{2020_Hsu,
author = {Li-Wen Hsu and Yu-Hsuan Lin and Jun-Yu Guo and Yi-Ju Chou and Chien Fu Chen and Yi Chun Yeh},
title = {Simultaneous Determination of l-Phenylalanine, Phenylethylamine, and Phenylacetic Acid Using Three-Color Whole-Cell Biosensors within a Microchannel Device},
journal = {ACS Applied Bio Materials},
year = {2020},
volume = {3},
publisher = {American Chemical Society (ACS)},
month = {jul},
url = {https://doi.org/10.1021/acsabm.0c00590},
number = {8},
pages = {5120--5125},
doi = {10.1021/acsabm.0c00590}
}
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Hsu, Li-Wen, et al. “Simultaneous Determination of l-Phenylalanine, Phenylethylamine, and Phenylacetic Acid Using Three-Color Whole-Cell Biosensors within a Microchannel Device.” ACS Applied Bio Materials, vol. 3, no. 8, Jul. 2020, pp. 5120-5125. https://doi.org/10.1021/acsabm.0c00590.