Open Access
ACS Omega, volume 4, issue 1, pages 2234-2240
Multiplex Viral Detection Platform Based on a Aptamers-Integrated Microfluidic Channel
1
University of Florida, Gainesville, Florida 32611, United States
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Publication type: Journal Article
Publication date: 2019-01-29
General Chemistry
General Chemical Engineering
Abstract
A polydimethylsiloxane-based microfluidic device has been developed for the multiplex detection of viral envelope proteins such as Zika and chikungunya on a single platform using aptamer-analyte interactions. The channel is integrated with microsized pillars that increase the surface area allowing more aptamers to attach to the incoming envelope protein molecules, thus increasing the overall sensitivity of the system. The working of the device depends on the formation of protein-mediated sandwich morphology that is obtained using an aptamer and aptamer-functionalized gold nanoparticle (AuNP) pair. The colorimetric signal is obtained upon introduction of silver reagents into the channel, which are selectively deposited on the AuNP surface, providing a gray contrast in the testing zone. The microfluidic channel approach successfully detected clinically relevant concentrations of Zika and chikungunya envelope proteins in phosphine-buffered saline (1 pM) and calf blood (100 pM) with high specificity using gold-decorated aptamers integrated in a microfluidic channel.
Top-30
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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Citations by publishers
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Elsevier
5 publications, 19.23%
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Multidisciplinary Digital Publishing Institute (MDPI)
4 publications, 15.38%
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4 publications, 15.38%
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Springer Nature
3 publications, 11.54%
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Wiley
2 publications, 7.69%
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American Chemical Society (ACS)
2 publications, 7.69%
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IEEE
1 publication, 3.85%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 3.85%
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Wolters Kluwer Health
1 publication, 3.85%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Saraf N. et al. Multiplex Viral Detection Platform Based on a Aptamers-Integrated Microfluidic Channel // ACS Omega. 2019. Vol. 4. No. 1. pp. 2234-2240.
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Saraf N., Villegas M., Willenberg B. J., Seal S. Multiplex Viral Detection Platform Based on a Aptamers-Integrated Microfluidic Channel // ACS Omega. 2019. Vol. 4. No. 1. pp. 2234-2240.
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TY - JOUR
DO - 10.1021/acsomega.8b03277
UR - https://doi.org/10.1021/acsomega.8b03277
TI - Multiplex Viral Detection Platform Based on a Aptamers-Integrated Microfluidic Channel
T2 - ACS Omega
AU - Saraf, Nileshi
AU - Villegas, Michael
AU - Seal, S.
AU - Willenberg, Bradley J
PY - 2019
DA - 2019/01/29 00:00:00
PB - American Chemical Society (ACS)
SP - 2234-2240
IS - 1
VL - 4
SN - 2470-1343
SN - 2470-1343
ER -
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@article{2019_Saraf,
author = {Nileshi Saraf and Michael Villegas and S. Seal and Bradley J Willenberg},
title = {Multiplex Viral Detection Platform Based on a Aptamers-Integrated Microfluidic Channel},
journal = {ACS Omega},
year = {2019},
volume = {4},
publisher = {American Chemical Society (ACS)},
month = {jan},
url = {https://doi.org/10.1021/acsomega.8b03277},
number = {1},
pages = {2234--2240},
doi = {10.1021/acsomega.8b03277}
}
Cite this
MLA
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Saraf, Nileshi, et al. “Multiplex Viral Detection Platform Based on a Aptamers-Integrated Microfluidic Channel.” ACS Omega, vol. 4, no. 1, Jan. 2019, pp. 2234-2240. https://doi.org/10.1021/acsomega.8b03277.