ACS Sensors, volume 5, issue 7, pages 2106-2117
Functionalized CdSe/ZnS Quantum Dots for Intracellular pH Measurements by Fluorescence Lifetime Imaging Microscopy
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Departamento de Química Física, Facultad de Farmacia, Universidad de Castilla-La Mancha, Av. Dr. José María Sánchez Ibáñez, s/n, 02071 Albacete, Spain
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2
Centro Regional de Investigaciones Biomédicas (CRIB), Unidad Asociada de Biomedicina (UCLM-CSIC), C/ Almansa, 14, 02008 Albacete, Spain
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3
Departamento de Química Inorgánica, Orgánica y Bioquímica, Facultad de Farmacia, Universidad de Castilla-La Mancha, Av. Dr. José María Sánchez Ibáñez, s/n, 02071 Albacete, Spain
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4
Departamento de Química Física, Facultad de Ciencias y Tecnologías Químicas, Universidad de Castilla-La Mancha, Avenida Camilo José Cela, 10, 13071 Ciudad Real, Spain
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Publication type: Journal Article
Publication date: 2020-06-18
Journal:
ACS Sensors
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 8.9
ISSN: 23793694, 23793694
Process Chemistry and Technology
Instrumentation
Bioengineering
Fluid Flow and Transfer Processes
Abstract
pH is an important biomarker for many human diseases and great efforts are being made to develop new pH probes for bioimaging and biomedical applications. Here, the use of three different CdSe/ZnS QDs, functionalized with d-penicillamine and small peptides, as pH probes for fluorescence lifetime imaging microscopy (FLIM) is investigated. The fluorescence pH sensitivity of the nanoparticles is analyzed in different experimental media: aqueous solution, synthetic intracellular medium, and mesenchymal C3H10T1/2 and tumoral SK-MEL-2 cell lines. Different experiments along with theoretical calculations are conducted to unravel the mechanisms causing pH sensitivity of the nanoparticles and the effect of the length and composition of the peripheral branches on their photophysical properties. Absolute intracellular pH values measured in live cells with FLIM using a fluorescent probe based on a QD are reported here for the first time (intracellular pH values of 7.0 and 7.1 for C3H10T1/2 and SK-MEL-2 cells, respectively). These fluorescent nanoprobes can also be used to distinguish between different types of cells in cocultures on the basis of their different fluorescence lifetimes in dissimilar intracellular environments.
Top-30
Citations by journals
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Journal of Photochemistry and Photobiology A: Chemistry
2 publications, 8.7%
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Pharmaceutics
1 publication, 4.35%
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Applied Sciences (Switzerland)
1 publication, 4.35%
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Nano Research
1 publication, 4.35%
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Biophysical Journal
1 publication, 4.35%
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Journal Physics D: Applied Physics
1 publication, 4.35%
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Chemical Engineering Journal
1 publication, 4.35%
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Environmental Pollution
1 publication, 4.35%
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Advanced Energy Materials
1 publication, 4.35%
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Nano Select
1 publication, 4.35%
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Journal of Physical Chemistry C
1 publication, 4.35%
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ACS applied materials & interfaces
1 publication, 4.35%
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Journal of Materials Chemistry B
1 publication, 4.35%
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Environmental Research
1 publication, 4.35%
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ACS Applied Bio Materials
1 publication, 4.35%
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TrAC - Trends in Analytical Chemistry
1 publication, 4.35%
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Analytical Chemistry
1 publication, 4.35%
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Biochemical and Biophysical Research Communications
1 publication, 4.35%
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Russian Chemical Reviews
1 publication, 4.35%
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Citations by publishers
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7
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Elsevier
7 publications, 30.43%
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American Chemical Society (ACS)
4 publications, 17.39%
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 8.7%
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Wiley
2 publications, 8.7%
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Springer Nature
1 publication, 4.35%
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Biophysical Society
1 publication, 4.35%
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IOP Publishing
1 publication, 4.35%
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Royal Society of Chemistry (RSC)
1 publication, 4.35%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 4.35%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Pacheco-Liñán P. J. et al. Functionalized CdSe/ZnS Quantum Dots for Intracellular pH Measurements by Fluorescence Lifetime Imaging Microscopy // ACS Sensors. 2020. Vol. 5. No. 7. pp. 2106-2117.
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Pacheco-Liñán P. J., Bravo I., Nueda M. L., Albaladejo J., GARZÓN A. Functionalized CdSe/ZnS Quantum Dots for Intracellular pH Measurements by Fluorescence Lifetime Imaging Microscopy // ACS Sensors. 2020. Vol. 5. No. 7. pp. 2106-2117.
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TY - JOUR
DO - 10.1021/acssensors.0c00719
UR - https://doi.org/10.1021/acssensors.0c00719
TI - Functionalized CdSe/ZnS Quantum Dots for Intracellular pH Measurements by Fluorescence Lifetime Imaging Microscopy
T2 - ACS Sensors
AU - Nueda, María L
AU - Pacheco-Liñán, Pedro J.
AU - Bravo, Ivan
AU - GARZÓN, ANDRÉS
AU - Albaladejo, J.
PY - 2020
DA - 2020/06/18 00:00:00
PB - American Chemical Society (ACS)
SP - 2106-2117
IS - 7
VL - 5
SN - 2379-3694
SN - 2379-3694
ER -
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@article{2020_Pacheco-Liñán,
author = {María L Nueda and Pedro J. Pacheco-Liñán and Ivan Bravo and ANDRÉS GARZÓN and J. Albaladejo},
title = {Functionalized CdSe/ZnS Quantum Dots for Intracellular pH Measurements by Fluorescence Lifetime Imaging Microscopy},
journal = {ACS Sensors},
year = {2020},
volume = {5},
publisher = {American Chemical Society (ACS)},
month = {jun},
url = {https://doi.org/10.1021/acssensors.0c00719},
number = {7},
pages = {2106--2117},
doi = {10.1021/acssensors.0c00719}
}
Cite this
MLA
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Pacheco-Liñán, Pedro J., et al. “Functionalized CdSe/ZnS Quantum Dots for Intracellular pH Measurements by Fluorescence Lifetime Imaging Microscopy.” ACS Sensors, vol. 5, no. 7, Jun. 2020, pp. 2106-2117. https://doi.org/10.1021/acssensors.0c00719.