ACS Sensors, volume 6, issue 6, pages 2378-2385
Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor
Publication type: Journal Article
Publication date: 2021-05-21
Journal:
ACS Sensors
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 8.9
ISSN: 23793694, 23793694
PubMed ID:
34019385
Process Chemistry and Technology
Instrumentation
Bioengineering
Fluid Flow and Transfer Processes
Abstract
We developed a new surface-enhanced Raman scattering (SERS)-based aptasensor platform capable of quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lysates with a high sensitivity. In this study, a spike protein deoxyribonucleic acid (DNA) aptamer was used as a receptor, and a self-grown Au nanopopcorn surface was used as a SERS detection substrate for the sensible detection of SARS-CoV-2. A quantitative analysis of the SARS-CoV-2 lysate was performed by monitoring the change in the SERS peak intensity caused by the new binding between the aptamer DNA released from the Au nanopopcorn surface and the spike protein in the SARS-CoV-2 virion. This technique enables detecting SARS-CoV-2 with a limit of detection (LoD) of less than 10 PFU/mL within 15 min. The results of this study demonstrate the possibility of a clinical application that can dramatically improve the detection limit and accuracy of the currently commercialized SARS-CoV-2 immunodiagnostic kit.
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Citations by publishers
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Elsevier
43 publications, 37.07%
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1 publication, 0.86%
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Frontiers Media S.A.
1 publication, 0.86%
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IOP Publishing
1 publication, 0.86%
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Bentham Science
1 publication, 0.86%
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Opto-Electronic Advances
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Hindawi Limited
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Cold Spring Harbor Laboratory
1 publication, 0.86%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 0.86%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Chen H. et al. Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor // ACS Sensors. 2021. Vol. 6. No. 6. pp. 2378-2385.
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Chen H., Park S., Choi N., Kwon H. J., Kang T., Lee M., Choo J. Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor // ACS Sensors. 2021. Vol. 6. No. 6. pp. 2378-2385.
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TY - JOUR
DO - 10.1021/acssensors.1c00596
UR - https://doi.org/10.1021/acssensors.1c00596
TI - Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor
T2 - ACS Sensors
AU - Kwon, Hyung Jun
AU - Park, Sung-Gyu
AU - Kang, Taejoon
AU - Choo, Jaebum
AU - Chen, Hao
AU - Choi, Namhyun
AU - Lee, Mikyung
PY - 2021
DA - 2021/05/21 00:00:00
PB - American Chemical Society (ACS)
SP - 2378-2385
IS - 6
VL - 6
PMID - 34019385
SN - 2379-3694
SN - 2379-3694
ER -
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@article{2021_Chen,
author = {Hyung Jun Kwon and Sung-Gyu Park and Taejoon Kang and Jaebum Choo and Hao Chen and Namhyun Choi and Mikyung Lee},
title = {Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor},
journal = {ACS Sensors},
year = {2021},
volume = {6},
publisher = {American Chemical Society (ACS)},
month = {may},
url = {https://doi.org/10.1021/acssensors.1c00596},
number = {6},
pages = {2378--2385},
doi = {10.1021/acssensors.1c00596}
}
Cite this
MLA
Copy
Chen, Hao, et al. “Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor.” ACS Sensors, vol. 6, no. 6, May. 2021, pp. 2378-2385. https://doi.org/10.1021/acssensors.1c00596.