Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor
Тип публикации: Journal Article
Дата публикации: 2021-05-21
scimago Q1
wos Q1
БС1
SJR: 1.757
CiteScore: 13.4
Impact factor: 9.1
ISSN: 23793694
PubMed ID:
34019385
Process Chemistry and Technology
Instrumentation
Bioengineering
Fluid Flow and Transfer Processes
Краткое описание
We developed a new surface-enhanced Raman scattering (SERS)-based aptasensor platform capable of quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lysates with a high sensitivity. In this study, a spike protein deoxyribonucleic acid (DNA) aptamer was used as a receptor, and a self-grown Au nanopopcorn surface was used as a SERS detection substrate for the sensible detection of SARS-CoV-2. A quantitative analysis of the SARS-CoV-2 lysate was performed by monitoring the change in the SERS peak intensity caused by the new binding between the aptamer DNA released from the Au nanopopcorn surface and the spike protein in the SARS-CoV-2 virion. This technique enables detecting SARS-CoV-2 with a limit of detection (LoD) of less than 10 PFU/mL within 15 min. The results of this study demonstrate the possibility of a clinical application that can dramatically improve the detection limit and accuracy of the currently commercialized SARS-CoV-2 immunodiagnostic kit.
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Метрики
148
Всего цитирований:
148
Цитирований c 2025:
22
(14.86%)
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ГОСТ |
RIS |
BibTex |
MLA
Цитировать
ГОСТ
Скопировать
Chen H. et al. Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor // ACS Sensors. 2021. Vol. 6. No. 6. pp. 2378-2385.
ГОСТ со всеми авторами (до 50)
Скопировать
Chen H., Park S., Choi N., Kwon H. J., Kang T., Lee M., Choo J. Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor // ACS Sensors. 2021. Vol. 6. No. 6. pp. 2378-2385.
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RIS
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TY - JOUR
DO - 10.1021/acssensors.1c00596
UR - https://doi.org/10.1021/acssensors.1c00596
TI - Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor
T2 - ACS Sensors
AU - Chen, Hao
AU - Park, Sung-Gyu
AU - Choi, Namhyun
AU - Kwon, Hyung Jun
AU - Kang, Taejoon
AU - Lee, Mikyung
AU - Choo, Jaebum
PY - 2021
DA - 2021/05/21
PB - American Chemical Society (ACS)
SP - 2378-2385
IS - 6
VL - 6
PMID - 34019385
SN - 2379-3694
ER -
Цитировать
BibTex (до 50 авторов)
Скопировать
@article{2021_Chen,
author = {Hao Chen and Sung-Gyu Park and Namhyun Choi and Hyung Jun Kwon and Taejoon Kang and Mikyung Lee and Jaebum Choo},
title = {Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor},
journal = {ACS Sensors},
year = {2021},
volume = {6},
publisher = {American Chemical Society (ACS)},
month = {may},
url = {https://doi.org/10.1021/acssensors.1c00596},
number = {6},
pages = {2378--2385},
doi = {10.1021/acssensors.1c00596}
}
Цитировать
MLA
Скопировать
Chen, Hao, et al. “Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor.” ACS Sensors, vol. 6, no. 6, May. 2021, pp. 2378-2385. https://doi.org/10.1021/acssensors.1c00596.