Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing
Тип публикации: Journal Article
Дата публикации: 2017-09-11
scimago Q1
wos Q1
БС1
SJR: 1.757
CiteScore: 13.4
Impact factor: 9.1
ISSN: 23793694
PubMed ID:
28884572
Process Chemistry and Technology
Instrumentation
Bioengineering
Fluid Flow and Transfer Processes
Краткое описание
Alzheimer's disease (AD) biomarkers can reflect the neurochemical indicators used to estimate the risk in clinical nephrology. Apolipoprotein E (ApoE) is an early biomarker for AD in clinical diagnosis. In this research, through bactrian camel immunization, lymphocyte isolation, RNA extraction, and library construction, ApoE-specific Nbs with high affinity were successfully separated from an immune phage display nanobody library. Herein, a colorimetric immunosensor was developed for the point-of-care testing of ApoE by layer-by-layer nanoassembly techniques and novel nanobodies (Nbs). Using highly oriented Nbs as the capture and detection antibodies, an on-site immunosensor was developed by detecting the mean gray value of fade color due to the glutaraldehyde@3-aminopropyltrimethoxysilane oxidation by H2O2. The detection limit of AopE is 0.42 pg/mL, and the clinical analysis achieves a good performance. The novel easily operated immunosensor may have potential application in the clinical diagnosis and real-time monitoring for AD.
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MLA
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ГОСТ
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Ren X. et al. Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing // ACS Sensors. 2017. Vol. 2. No. 9. pp. 1267-1271.
ГОСТ со всеми авторами (до 50)
Скопировать
Ren X., Yan J., Wu D., Wei Q., Wan Y. Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing // ACS Sensors. 2017. Vol. 2. No. 9. pp. 1267-1271.
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RIS
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TY - JOUR
DO - 10.1021/acssensors.7b00495
UR - https://doi.org/10.1021/acssensors.7b00495
TI - Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing
T2 - ACS Sensors
AU - Ren, Xiang
AU - Yan, Junrong
AU - Wu, Dan
AU - Wei, Qin
AU - Wan, Yakun
PY - 2017
DA - 2017/09/11
PB - American Chemical Society (ACS)
SP - 1267-1271
IS - 9
VL - 2
PMID - 28884572
SN - 2379-3694
ER -
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BibTex (до 50 авторов)
Скопировать
@article{2017_Ren,
author = {Xiang Ren and Junrong Yan and Dan Wu and Qin Wei and Yakun Wan},
title = {Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing},
journal = {ACS Sensors},
year = {2017},
volume = {2},
publisher = {American Chemical Society (ACS)},
month = {sep},
url = {https://doi.org/10.1021/acssensors.7b00495},
number = {9},
pages = {1267--1271},
doi = {10.1021/acssensors.7b00495}
}
Цитировать
MLA
Скопировать
Ren, Xiang, et al. “Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing.” ACS Sensors, vol. 2, no. 9, Sep. 2017, pp. 1267-1271. https://doi.org/10.1021/acssensors.7b00495.