Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing
1
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, China
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Publication type: Journal Article
Publication date: 2017-09-11
scimago Q1
wos Q1
SJR: 1.757
CiteScore: 13.4
Impact factor: 9.1
ISSN: 23793694
PubMed ID:
28884572
Process Chemistry and Technology
Instrumentation
Bioengineering
Fluid Flow and Transfer Processes
Abstract
Alzheimer's disease (AD) biomarkers can reflect the neurochemical indicators used to estimate the risk in clinical nephrology. Apolipoprotein E (ApoE) is an early biomarker for AD in clinical diagnosis. In this research, through bactrian camel immunization, lymphocyte isolation, RNA extraction, and library construction, ApoE-specific Nbs with high affinity were successfully separated from an immune phage display nanobody library. Herein, a colorimetric immunosensor was developed for the point-of-care testing of ApoE by layer-by-layer nanoassembly techniques and novel nanobodies (Nbs). Using highly oriented Nbs as the capture and detection antibodies, an on-site immunosensor was developed by detecting the mean gray value of fade color due to the glutaraldehyde@3-aminopropyltrimethoxysilane oxidation by H2O2. The detection limit of AopE is 0.42 pg/mL, and the clinical analysis achieves a good performance. The novel easily operated immunosensor may have potential application in the clinical diagnosis and real-time monitoring for AD.
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133
Total citations:
133
Citations from 2024:
8
(6.02%)
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GOST
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Ren X. et al. Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing // ACS Sensors. 2017. Vol. 2. No. 9. pp. 1267-1271.
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Ren X., Yan J., Wu D., Wei Q., Wan Y. Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing // ACS Sensors. 2017. Vol. 2. No. 9. pp. 1267-1271.
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TY - JOUR
DO - 10.1021/acssensors.7b00495
UR - https://doi.org/10.1021/acssensors.7b00495
TI - Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing
T2 - ACS Sensors
AU - Ren, Xiang
AU - Yan, Junrong
AU - Wu, Dan
AU - Wei, Qin
AU - Wan, Yakun
PY - 2017
DA - 2017/09/11
PB - American Chemical Society (ACS)
SP - 1267-1271
IS - 9
VL - 2
PMID - 28884572
SN - 2379-3694
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2017_Ren,
author = {Xiang Ren and Junrong Yan and Dan Wu and Qin Wei and Yakun Wan},
title = {Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing},
journal = {ACS Sensors},
year = {2017},
volume = {2},
publisher = {American Chemical Society (ACS)},
month = {sep},
url = {https://doi.org/10.1021/acssensors.7b00495},
number = {9},
pages = {1267--1271},
doi = {10.1021/acssensors.7b00495}
}
Cite this
MLA
Copy
Ren, Xiang, et al. “Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing.” ACS Sensors, vol. 2, no. 9, Sep. 2017, pp. 1267-1271. https://doi.org/10.1021/acssensors.7b00495.