Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine
Publication type: Journal Article
Publication date: 2018-11-12
scimago Q1
wos Q1
SJR: 1.241
CiteScore: 7.3
Impact factor: 3.9
ISSN: 21615063
PubMed ID:
30418753
General Medicine
Biomedical Engineering
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Abstract
Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.
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6
Total citations:
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Citations from 2024:
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(50%)
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Guo K. H., Lu K. H., Yeh Y. C. Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine // ACS Synthetic Biology. 2018. Vol. 7. No. 12. pp. 2790-2795.
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Guo K. H., Lu K. H., Yeh Y. C. Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine // ACS Synthetic Biology. 2018. Vol. 7. No. 12. pp. 2790-2795.
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RIS
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TY - JOUR
DO - 10.1021/acssynbio.8b00416
UR - https://doi.org/10.1021/acssynbio.8b00416
TI - Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine
T2 - ACS Synthetic Biology
AU - Guo, Kai Hong
AU - Lu, Kun Hua
AU - Yeh, Yi Chun
PY - 2018
DA - 2018/11/12
PB - American Chemical Society (ACS)
SP - 2790-2795
IS - 12
VL - 7
PMID - 30418753
SN - 2161-5063
ER -
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BibTex (up to 50 authors)
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@article{2018_Guo,
author = {Kai Hong Guo and Kun Hua Lu and Yi Chun Yeh},
title = {Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine},
journal = {ACS Synthetic Biology},
year = {2018},
volume = {7},
publisher = {American Chemical Society (ACS)},
month = {nov},
url = {https://doi.org/10.1021/acssynbio.8b00416},
number = {12},
pages = {2790--2795},
doi = {10.1021/acssynbio.8b00416}
}
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MLA
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Guo, Kai Hong, et al. “Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine.” ACS Synthetic Biology, vol. 7, no. 12, Nov. 2018, pp. 2790-2795. https://doi.org/10.1021/acssynbio.8b00416.