Peptide−Oligonucleotide Conjugates Form Stable and Selective Complexes with Antibody and DNA
Тип публикации: Journal Article
Дата публикации: 2008-06-05
SCImago Q1
WOS Q1
БС1
SJR: 0.947
CiteScore: 7
Impact factor: 4.5
ISSN: 10431802, 15204812
PubMed ID:
18529023
Organic Chemistry
Pharmacology
Pharmaceutical Science
Biotechnology
Bioengineering
Biomedical Engineering
Краткое описание
The present work demonstrates that the relatively low molecular weight synthetic peptide-oligonucleotide conjugates are capable of stable and selective three-component complex formation with complementary 72-100mer DNA oligonucleotides and a cardiac troponin I monoclonal antibody. Neither the Watson-Crick-type interaction between peptide-oligonucleotide conjugate and DNA nor the conjugate-antibody interaction dramatically hampers the other. These interactions remain selective and specific in the presence of several other conjugates not specific to cardiac troponin I monoclonal antibody as well as in the presence of control 100mer DNA oligonucleotides. The data herein demonstrate the feasibility of the synthetic peptide-oligonucleotide conjugates as convenient molecular tools, e.g., for antibody epitope mapping.
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Tennilä T. et al. Peptide−Oligonucleotide Conjugates Form Stable and Selective Complexes with Antibody and DNA // Bioconjugate Chemistry. 2008. Vol. 19. No. 7. pp. 1361-1367.
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Tennilä T., Antopolsky M., Azhayev A., Azhayeva E. Peptide−Oligonucleotide Conjugates Form Stable and Selective Complexes with Antibody and DNA // Bioconjugate Chemistry. 2008. Vol. 19. No. 7. pp. 1361-1367.
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TY - JOUR
DO - 10.1021/bc700457y
UR - https://doi.org/10.1021/bc700457y
TI - Peptide−Oligonucleotide Conjugates Form Stable and Selective Complexes with Antibody and DNA
T2 - Bioconjugate Chemistry
AU - Tennilä, Tuula
AU - Antopolsky, Maxim
AU - Azhayev, Alex
AU - Azhayeva, Elena
PY - 2008
DA - 2008/06/05
PB - American Chemical Society (ACS)
SP - 1361-1367
IS - 7
VL - 19
PMID - 18529023
SN - 1043-1802
SN - 1520-4812
ER -
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@article{2008_Tennilä,
author = {Tuula Tennilä and Maxim Antopolsky and Alex Azhayev and Elena Azhayeva},
title = {Peptide−Oligonucleotide Conjugates Form Stable and Selective Complexes with Antibody and DNA},
journal = {Bioconjugate Chemistry},
year = {2008},
volume = {19},
publisher = {American Chemical Society (ACS)},
month = {jun},
url = {https://doi.org/10.1021/bc700457y},
number = {7},
pages = {1361--1367},
doi = {10.1021/bc700457y}
}
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MLA
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Tennilä, Tuula, et al. “Peptide−Oligonucleotide Conjugates Form Stable and Selective Complexes with Antibody and DNA.” Bioconjugate Chemistry, vol. 19, no. 7, Jun. 2008, pp. 1361-1367. https://doi.org/10.1021/bc700457y.
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