Immobilization of Bovine Serum Albumin onto Porous Poly(vinylpyrrolidone)-Modified Silicas
Alexey V. Solomonov
1
,
Musabirov I.I.
2
,
S N Sergeev
2
,
Sergey Ivanov
3
,
Evgeniy V. Rumyantsev
1
,
Publication type: Journal Article
Publication date: 2014-08-20
scimago Q1
wos Q2
SJR: 0.828
CiteScore: 6.7
Impact factor: 3.9
ISSN: 08885885, 15205045
General Chemistry
General Chemical Engineering
Industrial and Manufacturing Engineering
Abstract
Grafting with poly(N-vinyl-2-pyrrolidone) (PVP) was used to functionalize silica. The functionalized silicas were characterized by scanning electron microscopy, nitrogen adsorption, energy-dispersive X-ray analysis, Fourier transform spectroscopy, and thermogravimetric analysis. The Barrett–Joyner–Halenda analysis confirmed that PVP-modified silica samples have a mesoporous structure. Bovine serum albumin (BSA) was immobilized onto the surface of PVP-modified silica systems through physical adsorption. Also it was shown that the adsorption capacity of the tested samples depends on the quantity of PVP inside the silica matrix, which confirmed the formation of a specific complex between BSA and PVP. In addition, the effects of pH, time, and concentration on BSA adsorption were investigated. The maximum BSA adsorption capacity was 71.54 ± 2.0 mg/g. The desorption and reusability of all prepared samples were studied as well. PVP-modified silicas are capable of holding more BSA compared to nonmodified silica. ...
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GOST
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Timin A. S. et al. Immobilization of Bovine Serum Albumin onto Porous Poly(vinylpyrrolidone)-Modified Silicas // Industrial & Engineering Chemistry Research. 2014. Vol. 53. No. 35. pp. 13699-13710.
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Timin A. S., Solomonov A. V., I.I. M., Sergeev S. N., Ivanov S., Rumyantsev E. V., Goncharenko A. Immobilization of Bovine Serum Albumin onto Porous Poly(vinylpyrrolidone)-Modified Silicas // Industrial & Engineering Chemistry Research. 2014. Vol. 53. No. 35. pp. 13699-13710.
Cite this
RIS
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TY - JOUR
DO - 10.1021/ie501915f
UR - https://doi.org/10.1021/ie501915f
TI - Immobilization of Bovine Serum Albumin onto Porous Poly(vinylpyrrolidone)-Modified Silicas
T2 - Industrial & Engineering Chemistry Research
AU - Timin, Alexander S.
AU - Solomonov, Alexey V.
AU - I.I., Musabirov
AU - Sergeev, S N
AU - Ivanov, Sergey
AU - Rumyantsev, Evgeniy V.
AU - Goncharenko, Alexander
PY - 2014
DA - 2014/08/20
PB - American Chemical Society (ACS)
SP - 13699-13710
IS - 35
VL - 53
SN - 0888-5885
SN - 1520-5045
ER -
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BibTex (up to 50 authors)
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@article{2014_Timin,
author = {Alexander S. Timin and Alexey V. Solomonov and Musabirov I.I. and S N Sergeev and Sergey Ivanov and Evgeniy V. Rumyantsev and Alexander Goncharenko},
title = {Immobilization of Bovine Serum Albumin onto Porous Poly(vinylpyrrolidone)-Modified Silicas},
journal = {Industrial & Engineering Chemistry Research},
year = {2014},
volume = {53},
publisher = {American Chemical Society (ACS)},
month = {aug},
url = {https://doi.org/10.1021/ie501915f},
number = {35},
pages = {13699--13710},
doi = {10.1021/ie501915f}
}
Cite this
MLA
Copy
Timin, Alexander S., et al. “Immobilization of Bovine Serum Albumin onto Porous Poly(vinylpyrrolidone)-Modified Silicas.” Industrial & Engineering Chemistry Research, vol. 53, no. 35, Aug. 2014, pp. 13699-13710. https://doi.org/10.1021/ie501915f.