Journal of the American Chemical Society, volume 130, issue 30, pages 9654-9655

Selective functionalization of a genetically encoded alkene-containing protein via "photoclick chemistry" in bacterial cells.

Publication typeJournal Article
Publication date2008-07-02
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor15
ISSN00027863, 15205126
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Abstract
We report a tetrazole-based, photoclick chemistry that can be employed to selectively functionalize an alkene genetically encoded in a protein inside E. coli cells. The reaction involved the treatment of E. coli cells with cell-permeable tetrazoles followed by a brief photo irradiation at 302 nm (4 min) and an overnight incubation at 4 degrees C. This in vivo alkene functionalization procedure was simple, straightforward, and nontoxic to E. coli cells. Additionally, fluorescent adducts were formed, facilitating the monitoring of the reaction in vivo. This reaction should offer a new tool for the study of alkene-containing proteins in living systems.

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GOST |
Cite this
GOST Copy
Song W. et al. Selective functionalization of a genetically encoded alkene-containing protein via "photoclick chemistry" in bacterial cells. // Journal of the American Chemical Society. 2008. Vol. 130. No. 30. pp. 9654-9655.
GOST all authors (up to 50) Copy
Song W., Wang Y., Qu J., Lin Q. Selective functionalization of a genetically encoded alkene-containing protein via "photoclick chemistry" in bacterial cells. // Journal of the American Chemical Society. 2008. Vol. 130. No. 30. pp. 9654-9655.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1021/ja803598e
UR - https://doi.org/10.1021/ja803598e
TI - Selective functionalization of a genetically encoded alkene-containing protein via "photoclick chemistry" in bacterial cells.
T2 - Journal of the American Chemical Society
AU - Song, Wenjiao
AU - Wang, Yizhong
AU - Qu, Jun
AU - Lin, Qing
PY - 2008
DA - 2008/07/02 00:00:00
PB - American Chemical Society (ACS)
SP - 9654-9655
IS - 30
VL - 130
SN - 0002-7863
SN - 1520-5126
ER -
BibTex |
Cite this
BibTex Copy
@article{2008_Song,
author = {Wenjiao Song and Yizhong Wang and Jun Qu and Qing Lin},
title = {Selective functionalization of a genetically encoded alkene-containing protein via "photoclick chemistry" in bacterial cells.},
journal = {Journal of the American Chemical Society},
year = {2008},
volume = {130},
publisher = {American Chemical Society (ACS)},
month = {jul},
url = {https://doi.org/10.1021/ja803598e},
number = {30},
pages = {9654--9655},
doi = {10.1021/ja803598e}
}
MLA
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MLA Copy
Song, Wenjiao, et al. “Selective functionalization of a genetically encoded alkene-containing protein via "photoclick chemistry" in bacterial cells..” Journal of the American Chemical Society, vol. 130, no. 30, Jul. 2008, pp. 9654-9655. https://doi.org/10.1021/ja803598e.
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