Journal of the American Chemical Society, volume 141, issue 4, pages 1425-1429
Deciphering Determinants in Ribosomal Methyltransferases That Confer Antimicrobial Resistance
Publication type: Journal Article
Publication date: 2019-01-09
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 15
ISSN: 00027863, 15205126
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Abstract
Post-translational methylation of rRNA at select positions is a prevalent resistance mechanism adopted by pathogens. In this work, KsgA, a housekeeping ribosomal methyltransferase (rMtase) involved in ribosome biogenesis, was exploited as a model system to delineate the specific targeting determinants that impart substrate specificity to rMtases. With a combination of evolutionary and structure-guided approaches, a set of chimeras were created that altered the targeting specificity of KsgA such that it acted similarly to erythromycin-resistant methyltransferases (Erms), rMtases found in multidrug-resistant pathogens. The results revealed that specific loop embellishments on the basic Rossmann fold are key determinants in the selection of the cognate RNA. Moreover, in vivo studies confirmed that chimeric constructs are competent in imparting macrolide resistance. This work explores the factors that govern the emergence of resistance and paves the way for the design of specific inhibitors useful in reversing antibiotic resistance.
Citations by journals
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Journal of Biological Chemistry
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Journal of Biosciences
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1 publication, 5.88%
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RNA
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1 publication, 5.88%
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Journal of Biomolecular Structure and Dynamics
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1 publication, 5.88%
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3
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Citations by publishers
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3
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Springer Nature
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Springer Nature
3 publications, 17.65%
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American Society for Biochemistry and Molecular Biology
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3 publications, 17.65%
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Pleiades Publishing
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1 publication, 5.88%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 5.88%
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Elsevier
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Elsevier
1 publication, 5.88%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
1 publication, 5.88%
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Royal Society of Chemistry (RSC)
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1 publication, 5.88%
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Oxford University Press
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1 publication, 5.88%
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Cold Spring Harbor Laboratory
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Taylor & Francis
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1 publication, 5.88%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Bhujbalrao R., Anand R. Deciphering Determinants in Ribosomal Methyltransferases That Confer Antimicrobial Resistance // Journal of the American Chemical Society. 2019. Vol. 141. No. 4. pp. 1425-1429.
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Bhujbalrao R., Anand R. Deciphering Determinants in Ribosomal Methyltransferases That Confer Antimicrobial Resistance // Journal of the American Chemical Society. 2019. Vol. 141. No. 4. pp. 1425-1429.
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TY - JOUR
DO - 10.1021/jacs.8b10277
UR - https://doi.org/10.1021%2Fjacs.8b10277
TI - Deciphering Determinants in Ribosomal Methyltransferases That Confer Antimicrobial Resistance
T2 - Journal of the American Chemical Society
AU - Bhujbalrao, Ruchika
AU - Anand, Ruchi
PY - 2019
DA - 2019/01/09 00:00:00
PB - American Chemical Society (ACS)
SP - 1425-1429
IS - 4
VL - 141
SN - 0002-7863
SN - 1520-5126
ER -
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@article{2019_Bhujbalrao,
author = {Ruchika Bhujbalrao and Ruchi Anand},
title = {Deciphering Determinants in Ribosomal Methyltransferases That Confer Antimicrobial Resistance},
journal = {Journal of the American Chemical Society},
year = {2019},
volume = {141},
publisher = {American Chemical Society (ACS)},
month = {jan},
url = {https://doi.org/10.1021%2Fjacs.8b10277},
number = {4},
pages = {1425--1429},
doi = {10.1021/jacs.8b10277}
}
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Bhujbalrao, Ruchika, and Ruchi Anand. “Deciphering Determinants in Ribosomal Methyltransferases That Confer Antimicrobial Resistance.” Journal of the American Chemical Society, vol. 141, no. 4, Jan. 2019, pp. 1425-1429. https://doi.org/10.1021%2Fjacs.8b10277.