Synthesis and Application of Fluorescein-Labeled Pluronic Block Copolymers to the Study of Polymer−Surface Interactions
Publication type: Journal Article
Publication date: 2000-12-22
scimago Q1
wos Q2
SJR: 0.763
CiteScore: 6.0
Impact factor: 3.9
ISSN: 07437463, 15205827
Spectroscopy
Electrochemistry
Condensed Matter Physics
General Materials Science
Surfaces and Interfaces
Abstract
We present a novel methodology for the conjugation of Pluronic poly(ethylene oxide)−poly(propylene oxide)−poly(ethylene oxide) block copolymers with a fluorescein derivative, 5-(4,6-dichlorotriazinyl) aminofluorescein (5-DTAF), at room temperature under aqueous conditions. Two Pluronic block copolymers, F68 with molecular weight (MW) 8400 and P105 with MW 6500, were examined. The labeled block copolymers exhibited maximum absorbance at 498 nm, peak fluorescence at 516 nm, and a marked reduction in both absorbance and fluorescence at acidic pH below 7. Flow cytometry was employed to quantify the real-time adsorption kinetics of labeled block copolymers to two types of surfaces: polystyrene microspheres and blood cells, specifically platelets. This methodology allowed detection of Pluronics at low solution concentrations down to 1 μM. In the case of labeled Pluronic F68 and P105 binding to polystyrene beads, a rapid time-dependent adsorption kinetics was observed with 80% adsorption occurring within 30 s a...
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Metrics
51
Total citations:
51
Citations from 2025:
2
(3.92%)
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MLA
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GOST
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Ahmed F., Alexandridis P., Neelamegham S. Synthesis and Application of Fluorescein-Labeled Pluronic Block Copolymers to the Study of Polymer−Surface Interactions // Langmuir. 2000. Vol. 17. No. 2. pp. 537-546.
GOST all authors (up to 50)
Copy
Ahmed F., Alexandridis P., Neelamegham S. Synthesis and Application of Fluorescein-Labeled Pluronic Block Copolymers to the Study of Polymer−Surface Interactions // Langmuir. 2000. Vol. 17. No. 2. pp. 537-546.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1021/la001305u
UR - https://doi.org/10.1021/la001305u
TI - Synthesis and Application of Fluorescein-Labeled Pluronic Block Copolymers to the Study of Polymer−Surface Interactions
T2 - Langmuir
AU - Ahmed, Fariyal
AU - Alexandridis, Paschalis
AU - Neelamegham, Sriram
PY - 2000
DA - 2000/12/22
PB - American Chemical Society (ACS)
SP - 537-546
IS - 2
VL - 17
SN - 0743-7463
SN - 1520-5827
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2000_Ahmed,
author = {Fariyal Ahmed and Paschalis Alexandridis and Sriram Neelamegham},
title = {Synthesis and Application of Fluorescein-Labeled Pluronic Block Copolymers to the Study of Polymer−Surface Interactions},
journal = {Langmuir},
year = {2000},
volume = {17},
publisher = {American Chemical Society (ACS)},
month = {dec},
url = {https://doi.org/10.1021/la001305u},
number = {2},
pages = {537--546},
doi = {10.1021/la001305u}
}
Cite this
MLA
Copy
Ahmed, Fariyal, et al. “Synthesis and Application of Fluorescein-Labeled Pluronic Block Copolymers to the Study of Polymer−Surface Interactions.” Langmuir, vol. 17, no. 2, Dec. 2000, pp. 537-546. https://doi.org/10.1021/la001305u.