Polyarginine Molecular Weight Determines Transfection Efficiency of Calcium Condensed Complexes
Publication type: Journal Article
Publication date: 2013-04-15
scimago Q1
wos Q1
SJR: 0.968
CiteScore: 7.8
Impact factor: 4.5
ISSN: 15438384, 15438392
PubMed ID:
23534410
Drug Discovery
Pharmaceutical Science
Molecular Medicine
Abstract
Cell penetrating peptides (CPPs) have been extensively studied in polyelectrolyte complexes as a means to enhance the transfection efficiency of plasmid DNA (pDNA). Increasing the molecular weight of CPPs often enhances gene expression but poses a risk of increased cytotoxicity and immunogenicity compared to low molecular weight CCPs. Conversely, low molecular weight CPPs typically have low transfection efficiency due to large complex size. Complexes made using low molecular weight CPPs were found to be condensed to a small size by adding calcium. In this study, complexes of low molecular weight polyarginine and pDNA were condensed with calcium. These complexes showed high transfection efficiency and low cytotoxicity in A549 carcinomic human alveolar basal epithelial cells. The relationships between transfection efficiency and polyarginine size (5, 7, 9, or 11 amino acids), polyarginine/pDNA charge ratios, and calcium concentrations were studied. Polyarginine 7 was significantly more effective than other polyarginines under most formulation conditions, suggesting a link between cell penetration ability and transfection efficiency.
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54
Total citations:
54
Citations from 2025:
2
(3.7%)
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MLA
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GOST
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Dhar P., Berkland C. Polyarginine Molecular Weight Determines Transfection Efficiency of Calcium Condensed Complexes // Molecular Pharmaceutics. 2013. Vol. 10. No. 5. pp. 1940-1948.
GOST all authors (up to 50)
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Dhar P., Berkland C. Polyarginine Molecular Weight Determines Transfection Efficiency of Calcium Condensed Complexes // Molecular Pharmaceutics. 2013. Vol. 10. No. 5. pp. 1940-1948.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1021/mp3007117
UR - https://doi.org/10.1021/mp3007117
TI - Polyarginine Molecular Weight Determines Transfection Efficiency of Calcium Condensed Complexes
T2 - Molecular Pharmaceutics
AU - Dhar, P.
AU - Berkland, Cory
PY - 2013
DA - 2013/04/15
PB - American Chemical Society (ACS)
SP - 1940-1948
IS - 5
VL - 10
PMID - 23534410
SN - 1543-8384
SN - 1543-8392
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2013_Dhar,
author = {P. Dhar and Cory Berkland},
title = {Polyarginine Molecular Weight Determines Transfection Efficiency of Calcium Condensed Complexes},
journal = {Molecular Pharmaceutics},
year = {2013},
volume = {10},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1021/mp3007117},
number = {5},
pages = {1940--1948},
doi = {10.1021/mp3007117}
}
Cite this
MLA
Copy
Dhar, P., and Cory Berkland. “Polyarginine Molecular Weight Determines Transfection Efficiency of Calcium Condensed Complexes.” Molecular Pharmaceutics, vol. 10, no. 5, Apr. 2013, pp. 1940-1948. https://doi.org/10.1021/mp3007117.