volume 36 issue 5 pages 693-697

The role of duplex ends in the spontaneous interaction of homologous linear DNA fragments

Publication typeJournal Article
Publication date2002-01-01
scimago Q3
wos Q4
SJR0.331
CiteScore1.6
Impact factor1.2
ISSN00268933, 16083245, 21689547
Structural Biology
Biophysics
Abstract
The spontaneous interaction of homologous linear DNA fragments was studied with a model of purified PCR products by agarose gel electrophoresis. To interact, duplexes required not only homology of internal regions, but also complementary ends. Fragments differing in terminal sequences did not interact. The yield of Holliday junctions (HJ), the simplest product of DNA–DNA interaction, depended on dissociation of fragment ends. Compared with genomic fragments, those with low-melting AT ends interacted with each other more efficiently and those with high-melting GC ends, less efficiently. Incubation temperature affected the equilibrium HJ concentration in solution of homologous fragments. A conclusion was made that HJ formation is initiated by nucleation of dissociated duplex ends.
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Markina V. et al. The role of duplex ends in the spontaneous interaction of homologous linear DNA fragments // Molecular Biology. 2002. Vol. 36. No. 5. pp. 693-697.
GOST all authors (up to 50) Copy
Markina V., Danilova O. A., Neschastnova A., Belitskii G. A., Yakubovskaya M. G. The role of duplex ends in the spontaneous interaction of homologous linear DNA fragments // Molecular Biology. 2002. Vol. 36. No. 5. pp. 693-697.
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RIS Copy
TY - JOUR
DO - 10.1023/A:1020679531678
UR - https://doi.org/10.1023/A:1020679531678
TI - The role of duplex ends in the spontaneous interaction of homologous linear DNA fragments
T2 - Molecular Biology
AU - Markina, V.K.
AU - Danilova, O. A.
AU - Neschastnova, A.A.
AU - Belitskii, G A
AU - Yakubovskaya, M G
PY - 2002
DA - 2002/01/01
PB - Pleiades Publishing
SP - 693-697
IS - 5
VL - 36
SN - 0026-8933
SN - 1608-3245
SN - 2168-9547
ER -
BibTex |
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BibTex (up to 50 authors) Copy
@article{2002_Markina,
author = {V.K. Markina and O. A. Danilova and A.A. Neschastnova and G A Belitskii and M G Yakubovskaya},
title = {The role of duplex ends in the spontaneous interaction of homologous linear DNA fragments},
journal = {Molecular Biology},
year = {2002},
volume = {36},
publisher = {Pleiades Publishing},
month = {jan},
url = {https://doi.org/10.1023/A:1020679531678},
number = {5},
pages = {693--697},
doi = {10.1023/A:1020679531678}
}
MLA
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Markina, V.K., et al. “The role of duplex ends in the spontaneous interaction of homologous linear DNA fragments.” Molecular Biology, vol. 36, no. 5, Jan. 2002, pp. 693-697. https://doi.org/10.1023/A:1020679531678.