Open Access
Cell Discovery, volume 7, issue 1, publication number 96
Isolation of a panel of ultra-potent human antibodies neutralizing SARS-CoV-2 and viral variants of concern
Andrey A Gorchakov
1
,
Sergey V Kulemzin
1
,
Sergey V Guselnikov
1
,
Konstantin O Baranov
1
,
Tatyana N Belovezhets
1
,
Ludmila V. Mechetina
1, 2
,
Olga Yu Volkova
1
,
Alexander M. Najakshin
1
,
Nikolai A Chikaev
1
,
Anton N Chikaev
1
,
Pavel P Solodkov
1
,
Victor F Larichev
3
,
Marina A Gulyaeva
2, 4
,
Alexander G Markhaev
4
,
Yulia V Kononova
4
,
Alexander Yu Alekseyev
4, 5
,
Alexander M. Shestopalov
4, 5
,
Gaukhar M Yusubalieva
6
,
Tatiana V Klypa
6
,
Vladimir T. Valuev-Elliston
7
,
Vladimir P. Baklaushev
6
,
6
Federal Research and Clinical Center for Specialized Medical Care, FMBA of Russia, Moscow, Russia
|
Publication type: Journal Article
Publication date: 2021-10-19
PubMed ID:
34667147
Biochemistry
Molecular Biology
Cell Biology
Genetics
Abstract
In the absence of virus-targeting small-molecule drugs approved for the treatment and prevention of COVID-19, broadening the repertoire of potent SARS-CoV-2-neutralizing antibodies represents an important area of research in response to the ongoing pandemic. Systematic analysis of such antibodies and their combinations can be particularly instrumental for identification of candidates that may prove resistant to the emerging viral escape variants. Here, we isolated a panel of 23 RBD-specific human monoclonal antibodies from the B cells of convalescent patients. A surprisingly large proportion of such antibodies displayed potent virus-neutralizing activity both in vitro and in vivo. Four of the isolated nAbs can be categorized as ultrapotent with an apparent IC100 below 16 ng/mL. We show that individual nAbs as well as dual combinations thereof retain activity against currently circulating SARS-CoV-2 variants of concern (such as B.1.1.7, B.1.351, B.1.617, and C.37), as well as against other viral variants. When used as a prophylactics or therapeutics, these nAbs could potently suppress viral replication and prevent lung pathology in SARS-CoV-2-infected hamsters. Our data contribute to the rational development of oligoclonal therapeutic nAb cocktails mitigating the risk of SARS-CoV-2 escape.
Top-30
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1 publication, 4%
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1 publication, 4%
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1 publication, 4%
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1 publication, 4%
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1 publication, 4%
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3
4
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Citations by publishers
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2
4
6
8
10
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Multidisciplinary Digital Publishing Institute (MDPI)
10 publications, 40%
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Springer Nature
6 publications, 24%
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Frontiers Media S.A.
3 publications, 12%
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Pleiades Publishing
1 publication, 4%
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Oxford University Press
1 publication, 4%
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Elsevier
1 publication, 4%
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1 publication, 4%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Gorchakov A. A. et al. Isolation of a panel of ultra-potent human antibodies neutralizing SARS-CoV-2 and viral variants of concern // Cell Discovery. 2021. Vol. 7. No. 1. 96
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Gorchakov A. A., Kulemzin S. V., Guselnikov S. V., Baranov K. O., Belovezhets T. N., Mechetina L. V., Volkova O. Yu., Najakshin A. M., Chikaev N. A., Chikaev A. N., Solodkov P. P., Larichev V. F., Gulyaeva M. A., Markhaev A. G., Kononova Y. V., Alekseyev A. Yu., Shestopalov A. M., Yusubalieva G. M., Klypa T. V., IVANOV A. V., Valuev-Elliston V. T., Baklaushev V. P., Taranin A. Isolation of a panel of ultra-potent human antibodies neutralizing SARS-CoV-2 and viral variants of concern // Cell Discovery. 2021. Vol. 7. No. 1. 96
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TY - JOUR
DO - 10.1038/s41421-021-00340-8
UR - https://doi.org/10.1038/s41421-021-00340-8
TI - Isolation of a panel of ultra-potent human antibodies neutralizing SARS-CoV-2 and viral variants of concern
T2 - Cell Discovery
AU - Gorchakov, Andrey A
AU - Kulemzin, Sergey V
AU - Guselnikov, Sergey V
AU - Baranov, Konstantin O
AU - Belovezhets, Tatyana N
AU - Mechetina, Ludmila V.
AU - Volkova, Olga Yu
AU - Najakshin, Alexander M.
AU - Chikaev, Nikolai A
AU - Chikaev, Anton N
AU - Solodkov, Pavel P
AU - Larichev, Victor F
AU - Gulyaeva, Marina A
AU - Markhaev, Alexander G
AU - Kononova, Yulia V
AU - Alekseyev, Alexander Yu
AU - Shestopalov, Alexander M.
AU - Yusubalieva, Gaukhar M
AU - Klypa, Tatiana V
AU - IVANOV, Alexander V.
AU - Valuev-Elliston, Vladimir T.
AU - Baklaushev, Vladimir P.
AU - Taranin, Alexander
PY - 2021
DA - 2021/10/19 00:00:00
PB - Springer Nature
IS - 1
VL - 7
PMID - 34667147
SN - 2056-5968
ER -
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@article{2021_Gorchakov,
author = {Andrey A Gorchakov and Sergey V Kulemzin and Sergey V Guselnikov and Konstantin O Baranov and Tatyana N Belovezhets and Ludmila V. Mechetina and Olga Yu Volkova and Alexander M. Najakshin and Nikolai A Chikaev and Anton N Chikaev and Pavel P Solodkov and Victor F Larichev and Marina A Gulyaeva and Alexander G Markhaev and Yulia V Kononova and Alexander Yu Alekseyev and Alexander M. Shestopalov and Gaukhar M Yusubalieva and Tatiana V Klypa and Alexander V. IVANOV and Vladimir T. Valuev-Elliston and Vladimir P. Baklaushev and Alexander Taranin},
title = {Isolation of a panel of ultra-potent human antibodies neutralizing SARS-CoV-2 and viral variants of concern},
journal = {Cell Discovery},
year = {2021},
volume = {7},
publisher = {Springer Nature},
month = {oct},
url = {https://doi.org/10.1038/s41421-021-00340-8},
number = {1},
doi = {10.1038/s41421-021-00340-8}
}
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