Cell Research, volume 33, issue 9, pages 661-678

Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids

Zongyong Ai ^{1, 2, 3}

Baohua Niu ^{1, 2}

Yu Yin ^{1, 2, 3}

Lifeng Xiang ⁴

Gaohui Shi ^{1, 2}

Kui Duan ^{1, 2, 3}

Sile Wang ^{1, 2, 3}

Yingjie Hu ^{1, 2}

Chi Zhang ^{1, 2}

Chengting Zhang ^{1, 2, 3}

Lujuan Rong ^{1, 2}

Ruize Kong ⁴

Tingwei Chen ^{1, 2}

Yixin Guo ⁵

Wanlu Liu ⁵

Nan Li ^{1, 2}

Shumei Zhao ^{1, 2, 3}

Xiaoqing Zhu ^{1, 2, 3}

Xuancheng Mai ⁴

Yonggang Li ⁴

Ze Wu ⁴

Yi Zheng ⁶

Jianping Fu ^{6, 7, 8}

Weizhi Ji ^{1, 2, 3}

Tianqing Li ^{1, 2, 3}

Show full list: 25 authors

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State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China |

Yunnan Key Laboratory of Primate Biomedical Research, Kunming, China |

Yunnan Provincial Academy of science and technology, Kunming, China |

⁴

Department of Reproductive Medicine, The First People’s Hospital of Yunnan Province, Kunming, China |

⁵

Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, China |

⁶

Department of Mechanical Engineering, University of Michigan, Ann Arbor, USA |

⁷

Department of Biomedical Engineering, University of Michigan, Ann Arbor, USA |

⁸

Department of Cell & Developmental Biology, University of Michigan Medical School, Ann Arbor, USA |

Publication type: Journal Article

Publication date: 2023-07-17

Springer Nature

Journal: Cell Research

scimago Q1

SJR: 9.506

CiteScore: 53.9

Impact factor: 28.1

ISSN: 10010602, 17487838

DOI: 10.1038/s41422-023-00846-8

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Molecular Biology

Cell Biology

Abstract

Studies of cultured embryos have provided insights into human peri-implantation development. However, detailed knowledge of peri-implantation lineage development as well as underlying mechanisms remains obscure. Using 3D-cultured human embryos, herein we report a complete cell atlas of the early post-implantation lineages and decipher cellular composition and gene signatures of the epiblast and hypoblast derivatives. In addition, we develop an embryo-like assembloid (E-assembloid) by assembling naive hESCs and extraembryonic cells. Using human embryos and E-assembloids, we reveal that WNT, BMP and Nodal signaling pathways synergistically, but functionally differently, orchestrate human peri-implantation lineage development. Specially, we dissect mechanisms underlying extraembryonic mesoderm and extraembryonic endoderm specifications. Finally, an improved E-assembloid is developed to recapitulate the epiblast and hypoblast development and tissue architectures in the pre-gastrulation human embryo. Our findings provide insights into human peri-implantation development, and the E-assembloid offers a useful model to disentangle cellular behaviors and signaling interactions that drive human embryogenesis.

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Publisher

Springer Nature

Journal

Cell Research

scimago Q1

SJR

9.506

CiteScore

53.9

Impact factor

28.1

ISSN

10010602 (Print)

17487838

Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids

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