Nature Medicine, volume 26, issue 9, pages 1422-1427

Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein

Seth J. Zost ¹

Pavlo Gilchuk ¹

Rita E. Chen ^{2, 3}

James Brett Case ³

Joseph X Reidy ¹

Andrew Trivette ¹

Rachel S. Nargi ¹

Rachel E. Sutton ¹

Naveenchandra Suryadevara ¹

Elaine C. Chen ⁴

Elad Binshtein ¹

Swathi Shrihari ³

Mario Ostrowski ⁵

Helen Y. Chu ⁶

Jonathan E. Didier ⁷

Keith W MacRenaris ⁷

Taylor Jones ¹

Samuel Day ¹

Luke Myers ¹

F. Eun-Hyung Lee ⁸

Doan C. Nguyen ⁸

Ignacio Sanz ⁸

David R. Martinez ⁹

Paul W Rothlauf ^{10, 11}

Louis Marie Bloyet ¹¹

Sean Whelan ^{10, 11}

Ralph S Baric ⁹

Larissa B Thackray ³

Michael S. Diamond ^{2, 3, 11, 12}

Robert H. Carnahan ^{1, 13}

James Crowe ^{1, 4, 13}

Hide authors affiliations Show authors affiliations: 13 affiliations

Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, USA |

Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, USA |

Department of Medicine, Washington University School of Medicine, St. Louis, USA |

⁴

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, USA |

⁵

Department of Medicine, University of Toronto, Toronto, CANADA |

⁶

Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA |

⁷

Berkeley Lights, Inc., Emeryville, USA |

⁸

Department of Medicine, Emory University, Atlanta, USA |

⁹

Department of Epidemiology, University of North Carolina At Chapel Hill, Chapel Hill, USA |

¹⁰

Program in Virology, Harvard Medical School, Boston, USA |

¹¹

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, USA |

¹²

Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, USA |

¹³

Department of Pediatrics, Vanderbilt University Medical Center, Nashville, USA |

Publication type: Journal Article

Publication date: 2020-07-10

Springer Nature

Journal: Nature Medicine

SJR: 19.045

CiteScore: 100.9

Impact factor: 58.7

ISSN: 10788956, 1546170X, 17447933

DOI: 10.1038/s41591-020-0998-x

Copy DOI

PubMed ID: 32651581

General Biochemistry, Genetics and Molecular Biology

General Medicine

Abstract

Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused a global pandemic with millions of infections and hundreds of thousands of deaths to date1,2. In response, we used a rapid antibody discovery platform to isolate hundreds of human monoclonal antibodies (mAbs) against the SARS-CoV-2 spike (S) protein. We stratify these mAbs into five major classes on the basis of their reactivity to subdomains of S protein as well as their cross-reactivity to SARS-CoV. Many of these mAbs inhibit infection of authentic SARS-CoV-2 virus, with most neutralizing mAbs recognizing the receptor-binding domain (RBD) of S. This work defines sites of vulnerability on SARS-CoV-2 S and demonstrates the speed and robustness of advanced antibody discovery platforms. A platform for rapid antibody discovery enabled the isolation of hundreds of human monoclonal antibodies against SARS-CoV-2 and the prioritization of potent antibody candidates for clinical trials in patients with COVID-19.

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GOST Copy

Zost S. J. et al. Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein // Nature Medicine. 2020. Vol. 26. No. 9. pp. 1422-1427.

GOST all authors (up to 50) Copy

Zost S. J., Gilchuk P., Chen R. E., Case J. B., Reidy J. X., Trivette A., Nargi R. S., Sutton R. E., Suryadevara N., Chen E. C., Binshtein E., Shrihari S., Ostrowski M., Chu H. Y., Didier J. E., MacRenaris K. W., Jones T., Day S., Myers L., Lee F. E., Nguyen D. C., Sanz I., Martinez D. R., Rothlauf P. W., Bloyet L. M., Whelan S., Baric R. S., Thackray L. B., Diamond M., Carnahan R. H., Crowe J. Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein // Nature Medicine. 2020. Vol. 26. No. 9. pp. 1422-1427.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1038/s41591-020-0998-x

UR - https://doi.org/10.1038/s41591-020-0998-x

TI - Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein

T2 - Nature Medicine

AU - Zost, Seth J.

AU - Gilchuk, Pavlo

AU - Chen, Rita E.

AU - Case, James Brett

AU - Reidy, Joseph X

AU - Trivette, Andrew

AU - Nargi, Rachel S.

AU - Sutton, Rachel E.

AU - Suryadevara, Naveenchandra

AU - Chen, Elaine C.

AU - Binshtein, Elad

AU - Shrihari, Swathi

AU - Ostrowski, Mario

AU - Chu, Helen Y.

AU - Didier, Jonathan E.

AU - MacRenaris, Keith W

AU - Jones, Taylor

AU - Day, Samuel

AU - Myers, Luke

AU - Lee, F. Eun-Hyung

AU - Nguyen, Doan C.

AU - Sanz, Ignacio

AU - Martinez, David R.

AU - Rothlauf, Paul W

AU - Bloyet, Louis Marie

AU - Whelan, Sean

AU - Baric, Ralph S

AU - Thackray, Larissa B

AU - Diamond, Michael S.

AU - Carnahan, Robert H.

AU - Crowe, James

PY - 2020

DA - 2020/07/10

PB - Springer Nature

SP - 1422-1427

IS - 9

VL - 26

PMID - 32651581

SN - 1078-8956

SN - 1546-170X

SN - 1744-7933

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2020_Zost,

author = {Seth J. Zost and Pavlo Gilchuk and Rita E. Chen and James Brett Case and Joseph X Reidy and Andrew Trivette and Rachel S. Nargi and Rachel E. Sutton and Naveenchandra Suryadevara and Elaine C. Chen and Elad Binshtein and Swathi Shrihari and Mario Ostrowski and Helen Y. Chu and Jonathan E. Didier and Keith W MacRenaris and Taylor Jones and Samuel Day and Luke Myers and F. Eun-Hyung Lee and Doan C. Nguyen and Ignacio Sanz and David R. Martinez and Paul W Rothlauf and Louis Marie Bloyet and Sean Whelan and Ralph S Baric and Larissa B Thackray and Michael S. Diamond and Robert H. Carnahan and James Crowe},

title = {Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein},

journal = {Nature Medicine},

year = {2020},

volume = {26},

publisher = {Springer Nature},

month = {jul},

url = {https://doi.org/10.1038/s41591-020-0998-x},

number = {9},

pages = {1422--1427},

doi = {10.1038/s41591-020-0998-x}

}

MLA

Cite this

MLA Copy

Zost, Seth J., et al. “Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein.” Nature Medicine, vol. 26, no. 9, Jul. 2020, pp. 1422-1427. https://doi.org/10.1038/s41591-020-0998-x.

Found error?

Publisher

Springer Nature

Journal

Nature Medicine

SJR

19.045

CiteScore

100.9

Impact factor

58.7

ISSN

10788956 (Print)

1546170X (Electronic)

17447933 (Electronic)