Open Access
Nature Communications, volume 4, issue 1, publication number 2796
VapC20 of Mycobacterium tuberculosis cleaves the sarcin-ricin loop of 23S rRNA.
1
Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle, UK
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3
Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle, UK
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Publication type: Journal Article
Publication date: 2013-11-14
Journal:
Nature Communications
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16.6
ISSN: 20411723
General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy
Abstract
The highly persistent and often lethal human pathogen, Mycobacterium tuberculosis contains at least 88 toxin–antitoxin genes. More than half of these encode VapC PIN domain endoribonucleases that inhibit cell growth by unknown mechanisms. Here we show that VapC20 of M. tuberculosis inhibits translation by cleavage of the Sarcin–Ricin loop (SRL) of 23S ribosomal RNA at the same position where Sarcin and other eukaryotic ribotoxins cleave. Toxin-inhibited cells can be rescued by the expression of the antitoxin, thereby raising the possibility that vapC20 contributes to the extreme persistence exhibited by M. tuberculosis. VapC20 cleavage is inhibited by mutations in the SRL that flank the cleavage site but not by changes elsewhere in the loop. Disruption of the SRL stem abolishes cleavage; however, further mutations that restore the SRL stem structure restore cleavage, revealing that the structure rather than the exact sequence of the SRL is important for this activity. Toxin–antitoxin systems have been implicated in the pathogenicity of Mycobacterium tuberculosis. Here, the authors study the function of the M. tuberculosistoxin VapC20 and show that it can impair protein translation and inhibit bacterial growth by cleaving the Sarcin–Ricin loop of 23S rRNA
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Winther K. S. et al. VapC20 of Mycobacterium tuberculosis cleaves the sarcin-ricin loop of 23S rRNA. // Nature Communications. 2013. Vol. 4. No. 1. 2796
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Winther K. S., Brodersen D. E., BROWN A. K., Gerdes K. VapC20 of Mycobacterium tuberculosis cleaves the sarcin-ricin loop of 23S rRNA. // Nature Communications. 2013. Vol. 4. No. 1. 2796
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TY - JOUR
DO - 10.1038/ncomms3796
UR - https://doi.org/10.1038%2Fncomms3796
TI - VapC20 of Mycobacterium tuberculosis cleaves the sarcin-ricin loop of 23S rRNA.
T2 - Nature Communications
AU - Winther, Kristoffer S
AU - Brodersen, Ditlev E.
AU - BROWN, Alistair K.
AU - Gerdes, Kenn
PY - 2013
DA - 2013/11/14 00:00:00
PB - Springer Nature
IS - 1
VL - 4
SN - 2041-1723
ER -
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@article{2013_Winther,
author = {Kristoffer S Winther and Ditlev E. Brodersen and Alistair K. BROWN and Kenn Gerdes},
title = {VapC20 of Mycobacterium tuberculosis cleaves the sarcin-ricin loop of 23S rRNA.},
journal = {Nature Communications},
year = {2013},
volume = {4},
publisher = {Springer Nature},
month = {nov},
url = {https://doi.org/10.1038%2Fncomms3796},
number = {1},
doi = {10.1038/ncomms3796}
}