Nature Methods, volume 7, issue 5, pages 377-381

Optimized localization analysis for single-molecule tracking and super-resolution microscopy

Kim I. Mortensen ¹

L Stirling Churchman ^{2, 3}

James A. Spudich ²

HENRIK FLYVBJERG ¹

Hide authors affiliations Show authors affiliations: 3 affiliations

Department of Micro- and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmark |

Department of Biochemistry, Stanford University School of Medicine, Stanford, USA |

Department of Physics, Stanford University School of Medicine, Stanford, USA |

Publication type: Journal Article

Publication date: 2010-04-04

Springer Nature

Journal: Nature Methods

Quartile SCImago

Quartile WOS

Impact factor: 48

ISSN: 15487091, 15487105

DOI: 10.1038/nmeth.1447

Copy DOI

Biochemistry

Molecular Biology

Cell Biology

Biotechnology

Abstract

A theoretical and experimental treatment of fitting methods for localizing the centers of diffraction-limited spots is presented. Use of an analytical point spread function shows that maximum likelihood fitting is superior to both unweighted and weighted least squares Gaussian fitting. We optimally localized isolated fluorescent beads and molecules imaged as diffraction-limited spots, determined the orientation of molecules and present reliable formulas for the precision of various localization methods. Both theory and experimental data showed that unweighted least-squares fitting of a Gaussian squanders one-third of the available information, a popular formula for its precision exaggerates beyond Fisher's information limit, and weighted least-squares may do worse, whereas maximum-likelihood fitting is practically optimal.

By date By citations

Top-30

Citations by journals

	5 10 15 20 25 30 35 40
Optics Express	Optics Express, 38, 5.29% Optics Express 38 publications, 5.29%
Nature Communications	Nature Communications, 23, 3.2% Nature Communications 23 publications, 3.2%
Nature Methods	Nature Methods, 21, 2.92% Nature Methods 21 publications, 2.92%
Biophysical Journal	Biophysical Journal, 20, 2.79% Biophysical Journal 20 publications, 2.79%
Scientific Reports	Scientific Reports, 19, 2.65% Scientific Reports 19 publications, 2.65%
PLoS ONE	PLoS ONE, 13, 1.81% PLoS ONE 13 publications, 1.81%
Nano Letters	Nano Letters, 13, 1.81% Nano Letters 13 publications, 1.81%
ACS Nano	ACS Nano, 12, 1.67% ACS Nano 12 publications, 1.67%
Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 12, 1.67% Proceedings of the National Academy of Sciences of the United States of America 12 publications, 1.67%
ChemPhysChem	ChemPhysChem, 10, 1.39% ChemPhysChem 10 publications, 1.39%
Chemical Reviews	Chemical Reviews, 10, 1.39% Chemical Reviews 10 publications, 1.39%
Methods in Molecular Biology	Methods in Molecular Biology, 10, 1.39% Methods in Molecular Biology 10 publications, 1.39%
Analytical Chemistry	Analytical Chemistry, 9, 1.25% Analytical Chemistry 9 publications, 1.25%
Physical Review Letters	Physical Review Letters, 9, 1.25% Physical Review Letters 9 publications, 1.25%
Angewandte Chemie	Angewandte Chemie, 8, 1.11% Angewandte Chemie 8 publications, 1.11%
Angewandte Chemie - International Edition	Angewandte Chemie - International Edition, 8, 1.11% Angewandte Chemie - International Edition 8 publications, 1.11%
Journal of the Optical Society of America A: Optics and Image Science, and Vision	Journal of the Optical Society of America A: Optics and Image Science, and Vision, 8, 1.11% Journal of the Optical Society of America A: Optics and Image Science, and Vision 8 publications, 1.11%
Methods	Methods, 7, 0.97% Methods 7 publications, 0.97%
ACS Photonics	ACS Photonics, 7, 0.97% ACS Photonics 7 publications, 0.97%
Physical Review E	Physical Review E, 6, 0.84% Physical Review E 6 publications, 0.84%
Histochemistry and Cell Biology	Histochemistry and Cell Biology, 6, 0.84% Histochemistry and Cell Biology 6 publications, 0.84%
Methods and Applications in Fluorescence	Methods and Applications in Fluorescence, 6, 0.84% Methods and Applications in Fluorescence 6 publications, 0.84%
Journal of Microscopy	Journal of Microscopy, 6, 0.84% Journal of Microscopy 6 publications, 0.84%
Nanoscale	Nanoscale, 6, 0.84% Nanoscale 6 publications, 0.84%
Physical Chemistry Chemical Physics	Physical Chemistry Chemical Physics, 6, 0.84% Physical Chemistry Chemical Physics 6 publications, 0.84%
Proceedings of SPIE - The International Society for Optical Engineering	Proceedings of SPIE - The International Society for Optical Engineering, 6, 0.84% Proceedings of SPIE - The International Society for Optical Engineering 6 publications, 0.84%
Science advances	Science advances, 6, 0.84% Science advances 6 publications, 0.84%
Journal of Cell Biology	Journal of Cell Biology, 5, 0.7% Journal of Cell Biology 5 publications, 0.7%
Biophysical Reviews	Biophysical Reviews, 5, 0.7% Biophysical Reviews 5 publications, 0.7%
	5 10 15 20 25 30 35 40

Citations by publishers

	20 40 60 80 100 120 140
Springer Nature	Springer Nature, 126, 17.55% Springer Nature 126 publications, 17.55%
American Chemical Society (ACS)	American Chemical Society (ACS), 74, 10.31% American Chemical Society (ACS) 74 publications, 10.31%
Wiley	Wiley, 66, 9.19% Wiley 66 publications, 9.19%
Elsevier	Elsevier, 63, 8.77% Elsevier 63 publications, 8.77%
Optical Society of America	Optical Society of America, 63, 8.77% Optical Society of America 63 publications, 8.77%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 58, 8.08% Cold Spring Harbor Laboratory 58 publications, 8.08%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 25, 3.48% Royal Society of Chemistry (RSC) 25 publications, 3.48%
Biophysical Society	Biophysical Society, 20, 2.79% Biophysical Society 20 publications, 2.79%
American Physical Society (APS)	American Physical Society (APS), 19, 2.65% American Physical Society (APS) 19 publications, 2.65%
IOP Publishing	IOP Publishing, 18, 2.51% IOP Publishing 18 publications, 2.51%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 15, 2.09% Public Library of Science (PLoS) 15 publications, 2.09%
American Institute of Physics (AIP)	American Institute of Physics (AIP), 13, 1.81% American Institute of Physics (AIP) 13 publications, 1.81%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 13, 1.81% Multidisciplinary Digital Publishing Institute (MDPI) 13 publications, 1.81%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 12, 1.67% Proceedings of the National Academy of Sciences (PNAS) 12 publications, 1.67%
IEEE	IEEE, 11, 1.53% IEEE 11 publications, 1.53%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 8, 1.11% American Association for the Advancement of Science (AAAS) 8 publications, 1.11%
Annual Reviews	Annual Reviews, 8, 1.11% Annual Reviews 8 publications, 1.11%
SPIE	SPIE, 7, 0.97% SPIE 7 publications, 0.97%
Frontiers Media S.A.	Frontiers Media S.A., 7, 0.97% Frontiers Media S.A. 7 publications, 0.97%
Oxford University Press	Oxford University Press, 7, 0.97% Oxford University Press 7 publications, 0.97%
Rockefeller University Press	Rockefeller University Press, 5, 0.7% Rockefeller University Press 5 publications, 0.7%
Taylor & Francis	Taylor & Francis, 5, 0.7% Taylor & Francis 5 publications, 0.7%
The Royal Society	The Royal Society, 4, 0.56% The Royal Society 4 publications, 0.56%
American Society for Cell Biology (ASCB)	American Society for Cell Biology (ASCB), 4, 0.56% American Society for Cell Biology (ASCB) 4 publications, 0.56%
Walter de Gruyter	Walter de Gruyter, 3, 0.42% Walter de Gruyter 3 publications, 0.42%
The Company of Biologists	The Company of Biologists, 3, 0.42% The Company of Biologists 3 publications, 0.42%
Portland Press	Portland Press, 3, 0.42% Portland Press 3 publications, 0.42%
World Scientific	World Scientific, 3, 0.42% World Scientific 3 publications, 0.42%
eLife Sciences Publications	eLife Sciences Publications, 3, 0.42% eLife Sciences Publications 3 publications, 0.42%
	20 40 60 80 100 120 140

We do not take into account publications without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2010,2011,2012,2013,2014,2015,2016,2017,2018,2019,2020,2021,2022,2023,2024],"ids":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","","","","","","","","",""],"datasets":[{"label":"Citations number","data":[8,24,37,47,66,61,58,61,71,53,48,65,57,44,18],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["1.11","3.34","5.15","6.55","9.19","8.5","8.08","8.5","9.89","7.38","6.69","9.05","7.94","6.13","2.51"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Optics Express","Nature Communications","Nature Methods","Biophysical Journal","Scientific Reports","PLoS ONE","Nano Letters","ACS Nano","Proceedings of the National Academy of Sciences of the United States of America","ChemPhysChem","Chemical Reviews","Methods in Molecular Biology","Analytical Chemistry","Physical Review Letters","Angewandte Chemie","Angewandte Chemie - International Edition","Journal of the Optical Society of America A: Optics and Image Science, and Vision","Methods","ACS Photonics","Physical Review E","Histochemistry and Cell Biology","Methods and Applications in Fluorescence","Journal of Microscopy","Nanoscale","Physical Chemistry Chemical Physics","Proceedings of SPIE - The International Society for Optical Engineering","Science advances","Journal of Cell Biology","Biophysical Reviews"],"ids":[8923,3231,14446,4026,13767,24963,10312,8724,306,16306,13718,3516,18157,4343,25450,19215,22383,16671,12730,12743,9446,9016,16289,228,1773,24723,23003,867,79],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/bypZPcr6C4twKiQVCUCGc0GF4cH6aUWmpClD3hsH_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/Bmz9XNb2QDptmPTa5N8huM54tShVewUj8fZqKFRy_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/nrK64iXHTzj43wMrfN1ZoUQ0vanswGzWPN45K3jA_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bypZPcr6C4twKiQVCUCGc0GF4cH6aUWmpClD3hsH_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/nrK64iXHTzj43wMrfN1ZoUQ0vanswGzWPN45K3jA_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/LsKy6OnmmmRGcAU6CZgWQvNiP1polbaSLNrN7zqj_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/DBpVkewJngs3BK2gVqHayOcCZf2Czbxddw6ObU26_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[38,23,21,20,19,13,13,12,12,10,10,10,9,9,8,8,8,7,7,6,6,6,6,6,6,6,6,5,5],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[5.29,3.2,2.92,2.79,2.65,1.81,1.81,1.67,1.67,1.39,1.39,1.39,1.25,1.25,1.11,1.11,1.11,0.97,0.97,0.84,0.84,0.84,0.84,0.84,0.84,0.84,0.84,0.7,0.7],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Springer Nature","American Chemical Society (ACS)","Wiley","Elsevier","Optical Society of America","Cold Spring Harbor Laboratory","Royal Society of Chemistry (RSC)","Biophysical Society","American Physical Society (APS)","IOP Publishing","Public Library of Science (PLoS)","American Institute of Physics (AIP)","Multidisciplinary Digital Publishing Institute (MDPI)","Proceedings of the National Academy of Sciences (PNAS)","IEEE","American Association for the Advancement of Science (AAAS)","Annual Reviews","SPIE","Frontiers Media S.A.","Oxford University Press","Rockefeller University Press","Taylor & Francis","The Royal Society","American Society for Cell Biology (ASCB)","Walter de Gruyter","The Company of Biologists","Portland Press","World Scientific","eLife Sciences Publications"],"ids":[8,40,11,17,375,6909,123,1454,1539,2075,344,250,202,162,6953,189,6941,7162,208,19,22,18,259,4151,4,872,801,23,6627],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bypZPcr6C4twKiQVCUCGc0GF4cH6aUWmpClD3hsH_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/Bmz9XNb2QDptmPTa5N8huM54tShVewUj8fZqKFRy_medium.webp","\/storage\/images\/resized\/nrK64iXHTzj43wMrfN1ZoUQ0vanswGzWPN45K3jA_medium.webp","\/storage\/images\/resized\/LsKy6OnmmmRGcAU6CZgWQvNiP1polbaSLNrN7zqj_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/ARM4e6URKRsbRZvIF0vFis9DjxGloBjnBYJXbHmZ_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/6scCJegesojp2jubwY3uKCzTAmgsaH2GIFlg6Hfk_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp","\/storage\/images\/resized\/DBpVkewJngs3BK2gVqHayOcCZf2Czbxddw6ObU26_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/BHBStD1OkQqtKFRRjS5njgNe7bXCAEgaDQVK7GTA_medium.webp","\/storage\/images\/resized\/1pFWD0EzQnc5egizpglbyNDhAbHCAVj1O7hBchfP_medium.webp","\/storage\/images\/resized\/3SpVxcYL33bOvPq4sHxJLH2NeKNeDloahSUpNiO4_medium.webp","\/storage\/images\/resized\/ko5EaisKGm66J1hHXtfEgVEeeWb9uOcMofpNuf0a_medium.webp","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/jZQkhOOcY9wrGwZm7XGRwMe8H7fThQu3KamISuun_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp"],"datasets":[{"label":"","data":[126,74,66,63,63,58,25,20,19,18,15,13,13,12,11,8,8,7,7,7,5,5,4,4,3,3,3,3,3],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[17.55,10.31,9.19,8.77,8.77,8.08,3.48,2.79,2.65,2.51,2.09,1.81,1.81,1.67,1.53,1.11,1.11,0.97,0.97,0.97,0.7,0.7,0.56,0.56,0.42,0.42,0.42,0.42,0.42],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartiles":{"type":"bar","data":{"show":true,"labels":[2010,2011,2012,2013,2014,2015,2016,2017,2018,2019,2020,2021,2022,2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,0,0,0,0,0,0,0,1,0,0,0,0,0,1],"percentage":["0","0","0","0","0","0","0","0","0.14","0","0","0","0","0","0.14"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,2,1,2,4,2,1,2,3,0,1,0,2,0,0],"percentage":["0","0.28","0.14","0.28","0.56","0.28","0.14","0.28","0.42","0","0.14","0","0.28","0","0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[0,3,2,8,5,7,11,6,8,2,3,7,4,4,1],"percentage":["0","0.42","0.28","1.11","0.7","0.97","1.53","0.84","1.11","0.28","0.42","0.97","0.56","0.56","0.14"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[7,17,26,31,43,45,41,35,48,34,32,47,40,26,13],"percentage":["0.97","2.37","3.62","4.32","5.99","6.27","5.71","4.87","6.69","4.74","4.46","6.55","5.57","3.62","1.81"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[1,2,8,6,14,7,5,18,11,17,12,11,11,14,3],"percentage":["0.14","0.28","1.11","0.84","1.95","0.97","0.7","2.51","1.53","2.37","1.67","1.53","1.53","1.95","0.42"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by SCImago per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartilesWs":{"type":"bar","data":{"show":true,"labels":[2010,2011,2012,2013,2014,2015,2016,2017,2018,2019,2020,2021,2022,2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,1,1,0,2,2,0,0,2,0,0,0,1,0,0],"percentage":["0","0.14","0.14","0","0.28","0.28","0","0","0.28","0","0","0","0.14","0","0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,4,1,2,4,7,6,4,5,3,1,6,5,3,2],"percentage":["0","0.56","0.14","0.28","0.56","0.97","0.84","0.56","0.7","0.42","0.14","0.84","0.7","0.42","0.28"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[4,8,14,21,22,20,18,16,18,10,9,13,9,10,3],"percentage":["0.56","1.11","1.95","2.92","3.06","2.79","2.51","2.23","2.51","1.39","1.25","1.81","1.25","1.39","0.42"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[2,9,12,16,22,23,27,18,32,19,23,30,25,17,9],"percentage":["0.28","1.25","1.67","2.23","3.06","3.2","3.76","2.51","4.46","2.65","3.2","4.18","3.48","2.37","1.25"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[2,2,9,8,16,9,7,23,14,21,15,16,17,14,4],"percentage":["0.28","0.28","1.25","1.11","2.23","1.25","0.97","3.2","1.95","2.92","2.09","2.23","2.37","1.95","0.56"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by WoS per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Are you a researcher?

Create a profile to get free access to personal recommendations for colleagues and new articles.

Metrics

Cite this

GOST |

Cite this

GOST Copy

Mortensen K. I. et al. Optimized localization analysis for single-molecule tracking and super-resolution microscopy // Nature Methods. 2010. Vol. 7. No. 5. pp. 377-381.

GOST all authors (up to 50) Copy

Mortensen K. I., Churchman L. S., Spudich J. A., FLYVBJERG H. Optimized localization analysis for single-molecule tracking and super-resolution microscopy // Nature Methods. 2010. Vol. 7. No. 5. pp. 377-381.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1038/nmeth.1447

UR - https://doi.org/10.1038/nmeth.1447

TI - Optimized localization analysis for single-molecule tracking and super-resolution microscopy

T2 - Nature Methods

AU - Mortensen, Kim I.

AU - Churchman, L Stirling

AU - Spudich, James A.

AU - FLYVBJERG, HENRIK

PY - 2010

DA - 2010/04/04 00:00:00

PB - Springer Nature

SP - 377-381

IS - 5

VL - 7

SN - 1548-7091

SN - 1548-7105

ER -

BibTex |

Cite this

BibTex Copy

@article{2010_Mortensen,

author = {Kim I. Mortensen and L Stirling Churchman and James A. Spudich and HENRIK FLYVBJERG},

title = {Optimized localization analysis for single-molecule tracking and super-resolution microscopy},

journal = {Nature Methods},

year = {2010},

volume = {7},

publisher = {Springer Nature},

month = {apr},

url = {https://doi.org/10.1038/nmeth.1447},

number = {5},

pages = {377--381},

doi = {10.1038/nmeth.1447}

}

MLA

Cite this

MLA Copy

Mortensen, Kim I., et al. “Optimized localization analysis for single-molecule tracking and super-resolution microscopy.” Nature Methods, vol. 7, no. 5, Apr. 2010, pp. 377-381. https://doi.org/10.1038/nmeth.1447.

Found error?

Publisher

Springer Nature

Journal

Nature Methods

Quartile SCImago

Quartile WOS

Impact factor

ISSN

15487091 (Print)
15487105 (Electronic)