Nature Methods, volume 2, issue 10, pages 743-749

Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots

Ben N. G. Giepmans ¹

Thomas J Deerinck ¹

Benjamin L. Smarr ¹

Ying Z. Jones ¹

Mark H. Ellisman ¹

Hide authors affiliations Show authors affiliations: 1 affiliation

Department of Neurosciences, National Center for Microscopy and Imaging Research, and Center for Research in Biological Systems, University of California, San Diego, La Jolla, USA |

Publication type: Journal Article

Publication date: 2005-09-22

Springer Nature

Journal: Nature Methods

Quartile SCImago

Quartile WOS

Impact factor: 48

ISSN: 15487091, 15487105

DOI: 10.1038/nmeth791

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Biochemistry

Molecular Biology

Cell Biology

Biotechnology

Abstract

The importance of locating proteins in their context within cells has been heightened recently by the accomplishments in molecular structure and systems biology. Although light microscopy (LM) has been extensively used for mapping protein localization, many studies require the additional resolution of the electron microscope. Here we report the application of small nanocrystals (Quantum dots; QDs) to specifically and efficiently label multiple distinct endogenous proteins. QDs are both fluorescent and electron dense, facilitating their use for correlated microscopic analysis. Furthermore, QDs can be discriminated optically by their emission wavelength and physically by size, making them invaluable for multilabeling analysis. We developed pre-embedding labeling criteria using QDs that allows optimization at the light level, before continuing with electron microscopy (EM). We provide examples of double and triple immunolabeling using light, electron and correlated microscopy in rat cells and mouse tissue. We conclude that QDs aid precise high-throughput determination of protein distribution.

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Citations by journals

	2 4 6 8 10 12 14
Methods in Cell Biology	Methods in Cell Biology, 13, 4.26% Methods in Cell Biology 13 publications, 4.26%
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Journal of Structural Biology	Journal of Structural Biology, 9, 2.95% Journal of Structural Biology 9 publications, 2.95%
Histochemistry and Cell Biology	Histochemistry and Cell Biology, 6, 1.97% Histochemistry and Cell Biology 6 publications, 1.97%
Journal of Microscopy	Journal of Microscopy, 6, 1.97% Journal of Microscopy 6 publications, 1.97%
Nano Letters	Nano Letters, 6, 1.97% Nano Letters 6 publications, 1.97%
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Nature Methods	Nature Methods, 4, 1.31% Nature Methods 4 publications, 1.31%
Small	Small, 4, 1.31% Small 4 publications, 1.31%
Methods in Molecular Biology	Methods in Molecular Biology, 4, 1.31% Methods in Molecular Biology 4 publications, 1.31%
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Microscopy and Microanalysis	Microscopy and Microanalysis, 4, 1.31% Microscopy and Microanalysis 4 publications, 1.31%
Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 4, 1.31% Proceedings of the National Academy of Sciences of the United States of America 4 publications, 1.31%
Microscopy Research and Technique	Microscopy Research and Technique, 3, 0.98% Microscopy Research and Technique 3 publications, 0.98%
Journal of Histochemistry and Cytochemistry	Journal of Histochemistry and Cytochemistry, 3, 0.98% Journal of Histochemistry and Cytochemistry 3 publications, 0.98%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 3, 0.98% International Journal of Molecular Sciences 3 publications, 0.98%
Nature Protocols	Nature Protocols, 3, 0.98% Nature Protocols 3 publications, 0.98%
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PLoS ONE	PLoS ONE, 3, 0.98% PLoS ONE 3 publications, 0.98%
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Chemical Reviews	Chemical Reviews, 3, 0.98% Chemical Reviews 3 publications, 0.98%
ACS Nano	ACS Nano, 3, 0.98% ACS Nano 3 publications, 0.98%
Nanoscale	Nanoscale, 3, 0.98% Nanoscale 3 publications, 0.98%
Nanomedicine	Nanomedicine, 2, 0.66% Nanomedicine 2 publications, 0.66%
Journal of Fluorescence	Journal of Fluorescence, 2, 0.66% Journal of Fluorescence 2 publications, 0.66%
Journal of Chemical Biology	Journal of Chemical Biology, 2, 0.66% Journal of Chemical Biology 2 publications, 0.66%
Nature Communications	Nature Communications, 2, 0.66% Nature Communications 2 publications, 0.66%
Protoplasma	Protoplasma, 2, 0.66% Protoplasma 2 publications, 0.66%
	2 4 6 8 10 12 14

Citations by publishers

	10 20 30 40 50 60 70 80
Elsevier	Elsevier, 71, 23.28% Elsevier 71 publications, 23.28%
Springer Nature	Springer Nature, 59, 19.34% Springer Nature 59 publications, 19.34%
Wiley	Wiley, 38, 12.46% Wiley 38 publications, 12.46%
American Chemical Society (ACS)	American Chemical Society (ACS), 19, 6.23% American Chemical Society (ACS) 19 publications, 6.23%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 9, 2.95% Royal Society of Chemistry (RSC) 9 publications, 2.95%
Oxford University Press	Oxford University Press, 9, 2.95% Oxford University Press 9 publications, 2.95%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 6, 1.97% Public Library of Science (PLoS) 6 publications, 1.97%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 5, 1.64% Cold Spring Harbor Laboratory 5 publications, 1.64%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 4, 1.31% Multidisciplinary Digital Publishing Institute (MDPI) 4 publications, 1.31%
Frontiers Media S.A.	Frontiers Media S.A., 4, 1.31% Frontiers Media S.A. 4 publications, 1.31%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 4, 1.31% Proceedings of the National Academy of Sciences (PNAS) 4 publications, 1.31%
Histochemical Society	Histochemical Society, 3, 0.98% Histochemical Society 3 publications, 0.98%
Taylor & Francis	Taylor & Francis, 3, 0.98% Taylor & Francis 3 publications, 0.98%
IEEE	IEEE, 3, 0.98% IEEE 3 publications, 0.98%
Future Medicine	Future Medicine, 2, 0.66% Future Medicine 2 publications, 0.66%
SAGE	SAGE, 2, 0.66% SAGE 2 publications, 0.66%
Cambridge University Press	Cambridge University Press, 2, 0.66% Cambridge University Press 2 publications, 0.66%
IOP Publishing	IOP Publishing, 2, 0.66% IOP Publishing 2 publications, 0.66%
Annual Reviews	Annual Reviews, 2, 0.66% Annual Reviews 2 publications, 0.66%
American Institute of Physics (AIP)	American Institute of Physics (AIP), 1, 0.33% American Institute of Physics (AIP) 1 publication, 0.33%
American Physical Society (APS)	American Physical Society (APS), 1, 0.33% American Physical Society (APS) 1 publication, 0.33%
The Company of Biologists	The Company of Biologists, 1, 0.33% The Company of Biologists 1 publication, 0.33%
Environmental Health Perspectives	Environmental Health Perspectives, 1, 0.33% Environmental Health Perspectives 1 publication, 0.33%
Portland Press	Portland Press, 1, 0.33% Portland Press 1 publication, 0.33%
The Royal Society	The Royal Society, 1, 0.33% The Royal Society 1 publication, 0.33%
Society of Nuclear Medicine and Molecular Imaging	Society of Nuclear Medicine and Molecular Imaging, 1, 0.33% Society of Nuclear Medicine and Molecular Imaging 1 publication, 0.33%
Future Science	Future Science, 1, 0.33% Future Science 1 publication, 0.33%
Biophysical Society of China	Biophysical Society of China, 1, 0.33% Biophysical Society of China 1 publication, 0.33%
Materials Research Society	Materials Research Society, 1, 0.33% Materials Research Society 1 publication, 0.33%
	10 20 30 40 50 60 70 80

We do not take into account publications without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

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Giepmans B. G. et al. Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots // Nature Methods. 2005. Vol. 2. No. 10. pp. 743-749.

GOST all authors (up to 50) Copy

Giepmans B. G., Deerinck T. J., Smarr B. L., Jones Y. Z., Ellisman M. H. Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots // Nature Methods. 2005. Vol. 2. No. 10. pp. 743-749.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1038/nmeth791

UR - https://doi.org/10.1038/nmeth791

TI - Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots

T2 - Nature Methods

AU - Giepmans, Ben N. G.

AU - Deerinck, Thomas J

AU - Smarr, Benjamin L.

AU - Jones, Ying Z.

AU - Ellisman, Mark H.

PY - 2005

DA - 2005/09/22 00:00:00

PB - Springer Nature

SP - 743-749

IS - 10

VL - 2

SN - 1548-7091

SN - 1548-7105

ER -

BibTex |

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BibTex Copy

@article{2005_Giepmans,

author = {Ben N. G. Giepmans and Thomas J Deerinck and Benjamin L. Smarr and Ying Z. Jones and Mark H. Ellisman},

title = {Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots},

journal = {Nature Methods},

year = {2005},

volume = {2},

publisher = {Springer Nature},

month = {sep},

url = {https://doi.org/10.1038/nmeth791},

number = {10},

pages = {743--749},

doi = {10.1038/nmeth791}

}

MLA

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MLA Copy

Giepmans, Ben N. G., et al. “Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots.” Nature Methods, vol. 2, no. 10, Sep. 2005, pp. 743-749. https://doi.org/10.1038/nmeth791.

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Springer Nature

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Nature Methods

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15487091 (Print)
15487105 (Electronic)