Open Access
Nature Nanotechnology, volume 4, issue 5, pages 325-330
A DNA nanomachine that maps spatial and temporal pH changes inside living cells
Publication type: Journal Article
Publication date: 2009-04-06
Journal:
Nature Nanotechnology
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 38.3
ISSN: 17483387, 17483395
Atomic and Molecular Physics, and Optics
Condensed Matter Physics
General Materials Science
Electrical and Electronic Engineering
Bioengineering
Biomedical Engineering
Abstract
DNA nanomachines are synthetic assemblies that switch between defined molecular conformations upon stimulation by external triggers. Previously, the performance of DNA devices has been limited to in vitro applications. Here we report the construction of a DNA nanomachine called the I-switch, which is triggered by protons and functions as a pH sensor based on fluorescence resonance energy transfer (FRET) inside living cells. It is an efficient reporter of pH from pH 5.5 to 6.8, with a high dynamic range between pH 5.8 and 7. To demonstrate its ability to function inside living cells we use the I-switch to map spatial and temporal pH changes associated with endosome maturation. The performance of our DNA nanodevices inside living systems illustrates the potential of DNA scaffolds responsive to more complex triggers in sensing, diagnostics and targeted therapies in living systems.
Citations by journals
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Angewandte Chemie - International Edition
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ACS Nano
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12 publications, 1.8%
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Nano Letters
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5 publications, 0.75%
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Nature Communications
5 publications, 0.75%
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Sensors and Actuators, B: Chemical
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Sensors and Actuators, B: Chemical
5 publications, 0.75%
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The Analyst
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5 publications, 0.75%
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Physical Chemistry Chemical Physics
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Physical Chemistry Chemical Physics
5 publications, 0.75%
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Citations by publishers
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Wiley
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Wiley
157 publications, 23.54%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
144 publications, 21.59%
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Royal Society of Chemistry (RSC)
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Royal Society of Chemistry (RSC)
93 publications, 13.94%
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Springer Nature
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Springer Nature
65 publications, 9.75%
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Elsevier
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65 publications, 9.75%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
13 publications, 1.95%
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Oxford University Press
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Oxford University Press
13 publications, 1.95%
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IOP Publishing
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IOP Publishing
8 publications, 1.2%
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American Association for the Advancement of Science (AAAS)
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American Association for the Advancement of Science (AAAS)
5 publications, 0.75%
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Taylor & Francis
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Taylor & Francis
4 publications, 0.6%
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Proceedings of the National Academy of Sciences (PNAS)
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Proceedings of the National Academy of Sciences (PNAS)
4 publications, 0.6%
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The Royal Society
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The Royal Society
3 publications, 0.45%
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Public Library of Science (PLoS)
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Public Library of Science (PLoS)
3 publications, 0.45%
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eLife Sciences Publications
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3 publications, 0.45%
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Annual Reviews
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3 publications, 0.45%
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Frontiers Media S.A.
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2 publications, 0.3%
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Biophysical Society
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Biophysical Society
2 publications, 0.3%
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2 publications, 0.3%
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IEEE
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IEEE
2 publications, 0.3%
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Hindawi Limited
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Hindawi Limited
2 publications, 0.3%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 0.15%
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Bentham Science
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Bentham Science
1 publication, 0.15%
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The Company of Biologists
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The Company of Biologists
1 publication, 0.15%
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The Chemical Society of Japan
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The Chemical Society of Japan
1 publication, 0.15%
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Mary Ann Liebert
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Mary Ann Liebert
1 publication, 0.15%
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SPIE
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SPIE
1 publication, 0.15%
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World Scientific
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World Scientific
1 publication, 0.15%
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Cambridge University Press
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Cambridge University Press
1 publication, 0.15%
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King Saud University
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King Saud University
1 publication, 0.15%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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GOST
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Modi S. et al. A DNA nanomachine that maps spatial and temporal pH changes inside living cells // Nature Nanotechnology. 2009. Vol. 4. No. 5. pp. 325-330.
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Modi S., M G S., Goswami D., Gupta G. D., Mayor S., Krishnan Y. A DNA nanomachine that maps spatial and temporal pH changes inside living cells // Nature Nanotechnology. 2009. Vol. 4. No. 5. pp. 325-330.
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RIS
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TY - JOUR
DO - 10.1038/nnano.2009.83
UR - https://doi.org/10.1038%2Fnnano.2009.83
TI - A DNA nanomachine that maps spatial and temporal pH changes inside living cells
T2 - Nature Nanotechnology
AU - Modi, Souvik
AU - M G, Swetha
AU - Goswami, Debanjan
AU - Gupta, Gagan D.
AU - Mayor, Satyajit
AU - Krishnan, Yamuna
PY - 2009
DA - 2009/04/06 00:00:00
PB - Springer Nature
SP - 325-330
IS - 5
VL - 4
SN - 1748-3387
SN - 1748-3395
ER -
Cite this
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@article{2009_Modi,
author = {Souvik Modi and Swetha M G and Debanjan Goswami and Gagan D. Gupta and Satyajit Mayor and Yamuna Krishnan},
title = {A DNA nanomachine that maps spatial and temporal pH changes inside living cells},
journal = {Nature Nanotechnology},
year = {2009},
volume = {4},
publisher = {Springer Nature},
month = {apr},
url = {https://doi.org/10.1038%2Fnnano.2009.83},
number = {5},
pages = {325--330},
doi = {10.1038/nnano.2009.83}
}
Cite this
MLA
Copy
Modi, Souvik, et al. “A DNA nanomachine that maps spatial and temporal pH changes inside living cells.” Nature Nanotechnology, vol. 4, no. 5, Apr. 2009, pp. 325-330. https://doi.org/10.1038%2Fnnano.2009.83.