O-Pair Search with MetaMorpheus for O-glycopeptide characterization
Lei Lu
1
,
Nicholas M Riley
2
,
Michael R. Shortreed
1
,
Carolyn R. Bertozzi
2, 3
,
Lloyd V. Smith
1
Publication type: Journal Article
Publication date: 2020-10-26
scimago Q1
wos Q1
SJR: 17.251
CiteScore: 49.0
Impact factor: 32.1
ISSN: 15487091, 15487105
PubMed ID:
33106676
Biochemistry
Molecular Biology
Cell Biology
Biotechnology
Abstract
We report O-Pair Search, an approach to identify O-glycopeptides and localize O-glycosites. Using paired collision- and electron-based dissociation spectra, O-Pair Search identifies O-glycopeptides via an ion-indexed open modification search and localizes O-glycosites using graph theory and probability-based localization. O-Pair Search reduces search times more than 2,000-fold compared to current O-glycopeptide processing software, while defining O-glycosite localization confidence levels and generating more O-glycopeptide identifications. Beyond the mucin-type O-glycopeptides discussed here, O-Pair Search also accepts user-defined glycan databases, making it compatible with many types of O-glycosylation. O-Pair Search is freely available within the open-source MetaMorpheus platform at https://github.com/smith-chem-wisc/MetaMorpheus . O-Pair search identifies O-glycopeptides and localizes O-glycosites using a fragment-ion-indexed open modification search combined with a graph-based approach. It also introduces a classification scheme to unify data reporting for glycoproteomics.
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Total citations:
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Citations from 2024:
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Lu L. et al. O-Pair Search with MetaMorpheus for O-glycopeptide characterization // Nature Methods. 2020. Vol. 17. No. 11. pp. 1133-1138.
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Lu L., Riley N. M., Shortreed M. R., Bertozzi C. R., Smith L. V. O-Pair Search with MetaMorpheus for O-glycopeptide characterization // Nature Methods. 2020. Vol. 17. No. 11. pp. 1133-1138.
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TY - JOUR
DO - 10.1038/s41592-020-00985-5
UR - https://doi.org/10.1038/s41592-020-00985-5
TI - O-Pair Search with MetaMorpheus for O-glycopeptide characterization
T2 - Nature Methods
AU - Lu, Lei
AU - Riley, Nicholas M
AU - Shortreed, Michael R.
AU - Bertozzi, Carolyn R.
AU - Smith, Lloyd V.
PY - 2020
DA - 2020/10/26
PB - Springer Nature
SP - 1133-1138
IS - 11
VL - 17
PMID - 33106676
SN - 1548-7091
SN - 1548-7105
ER -
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BibTex (up to 50 authors)
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@article{2020_Lu,
author = {Lei Lu and Nicholas M Riley and Michael R. Shortreed and Carolyn R. Bertozzi and Lloyd V. Smith},
title = {O-Pair Search with MetaMorpheus for O-glycopeptide characterization},
journal = {Nature Methods},
year = {2020},
volume = {17},
publisher = {Springer Nature},
month = {oct},
url = {https://doi.org/10.1038/s41592-020-00985-5},
number = {11},
pages = {1133--1138},
doi = {10.1038/s41592-020-00985-5}
}
Cite this
MLA
Copy
Lu, Lei, et al. “O-Pair Search with MetaMorpheus for O-glycopeptide characterization.” Nature Methods, vol. 17, no. 11, Oct. 2020, pp. 1133-1138. https://doi.org/10.1038/s41592-020-00985-5.