Open Access
Scientific Reports, volume 10, issue 1, publication number 2677
N-terminal domain of the architectural protein CTCF has similar structural organization and ability to self-association in bilaterian organisms
Bonchuk Artem
1, 2
,
Kamalyan Sofia
2, 3
,
Mariasina Sofia
4, 5
,
Boyko Konstantin
6
,
Popov V.V.
6, 7
,
Maksimenko Oksana
1
,
Georgiev Pavel
2
6
Publication type: Journal Article
Publication date: 2020-02-14
PubMed ID:
32060375
Multidisciplinary
Abstract
CTCF is the main architectural protein found in most of the examined bilaterian organisms. The cluster of the C2H2 zinc-finger domains involved in recognition of long DNA-binding motif is only part of the protein that is evolutionarily conserved, while the N-terminal domain (NTD) has different sequences. Here, we performed biophysical characterization of CTCF NTDs from various species representing all major phylogenetic clades of higher metazoans. With the exception of Drosophilides, the N-terminal domains of CTCFs show an unstructured organization and absence of folded regions in vitro. In contrast, NTDs of Drosophila melanogaster and virilis CTCFs contain unstructured folded regions that form tetramers and dimers correspondingly in vitro. Unexpectedly, most NTDs are able to self-associate in the yeast two-hybrid and co-immunoprecipitation assays. These results suggest that NTDs of CTCFs might contribute to the organization of CTCF-mediated long-distance interactions and chromosomal architecture.
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Citations by publishers
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Springer Nature
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1 publication, 5.56%
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Taylor & Francis
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Bonchuk A. et al. N-terminal domain of the architectural protein CTCF has similar structural organization and ability to self-association in bilaterian organisms // Scientific Reports. 2020. Vol. 10. No. 1. 2677
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Bonchuk A., Kamalyan S., Mariasina S., Boyko K., Popov V., Maksimenko O., Georgiev P. N-terminal domain of the architectural protein CTCF has similar structural organization and ability to self-association in bilaterian organisms // Scientific Reports. 2020. Vol. 10. No. 1. 2677
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TY - JOUR
DO - 10.1038/s41598-020-59459-5
UR - https://doi.org/10.1038%2Fs41598-020-59459-5
TI - N-terminal domain of the architectural protein CTCF has similar structural organization and ability to self-association in bilaterian organisms
T2 - Scientific Reports
AU - Kamalyan, Sofia
AU - Mariasina, Sofia
AU - Boyko, Konstantin
AU - Bonchuk, Artem
AU - Popov, V.V.
AU - Maksimenko, Oksana
AU - Georgiev, Pavel
PY - 2020
DA - 2020/02/14 00:00:00
PB - Springer Nature
IS - 1
VL - 10
PMID - 32060375
SN - 2045-2322
ER -
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@article{2020_Bonchuk,
author = {Sofia Kamalyan and Sofia Mariasina and Konstantin Boyko and Artem Bonchuk and V.V. Popov and Oksana Maksimenko and Pavel Georgiev},
title = {N-terminal domain of the architectural protein CTCF has similar structural organization and ability to self-association in bilaterian organisms},
journal = {Scientific Reports},
year = {2020},
volume = {10},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1038%2Fs41598-020-59459-5},
number = {1},
doi = {10.1038/s41598-020-59459-5}
}