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Scientific Reports, volume 11, issue 1, publication number 18241

Novel cryo-EM structure of an ADP-bound GroEL–GroES complex

Kudryavtseva Sofia S 1, 2
Pichkur Evgeny B 3, 4
Yaroshevich Igor A 1
Mamchur Aleksandra A 1
Panina Irina S 5
Moiseenko Andrei V 1
Muronetz Vladimir I. 2, 6
Stanishneva Konovalova Tatiana B 1
Publication typeJournal Article
Publication date2021-09-14
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor4.6
ISSN20452322
Multidisciplinary
Abstract

The GroEL–GroES chaperonin complex is a bacterial protein folding system, functioning in an ATP-dependent manner. Upon ATP binding and hydrolysis, it undergoes multiple stages linked to substrate protein binding, folding and release. Structural methods helped to reveal several conformational states and provide more information about the chaperonin functional cycle. Here, using cryo-EM we resolved two nucleotide-bound structures of the bullet-shaped GroEL–GroES1 complex at 3.4 Å resolution. The main difference between them is the relative orientation of their apical domains. Both structures contain nucleotides in cis and trans GroEL rings; in contrast to previously reported bullet-shaped complexes where nucleotides were only present in the cis ring. Our results suggest that the bound nucleotides correspond to ADP, and that such a state appears at low ATP:ADP ratios.

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Kudryavtseva S. S. et al. Novel cryo-EM structure of an ADP-bound GroEL–GroES complex // Scientific Reports. 2021. Vol. 11. No. 1. 18241
GOST all authors (up to 50) Copy
Kudryavtseva S. S., Pichkur E. B., Yaroshevich I. A., Mamchur A. A., Panina I. S., Moiseenko A. V., Sokolova O. S., Muronetz V. I., Stanishneva Konovalova T. B. Novel cryo-EM structure of an ADP-bound GroEL–GroES complex // Scientific Reports. 2021. Vol. 11. No. 1. 18241
RIS |
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RIS Copy
TY - JOUR
DO - 10.1038/s41598-021-97657-x
UR - https://doi.org/10.1038%2Fs41598-021-97657-x
TI - Novel cryo-EM structure of an ADP-bound GroEL–GroES complex
T2 - Scientific Reports
AU - Kudryavtseva, Sofia S
AU - Yaroshevich, Igor A
AU - Mamchur, Aleksandra A
AU - Panina, Irina S
AU - Moiseenko, Andrei V
AU - Sokolova, Olga S.
AU - Muronetz, Vladimir I.
AU - Stanishneva Konovalova, Tatiana B
AU - Pichkur, Evgeny B
PY - 2021
DA - 2021/09/14 00:00:00
PB - Springer Nature
IS - 1
VL - 11
PMID - 34521893
SN - 2045-2322
ER -
BibTex
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BibTex Copy
@article{2021_Kudryavtseva
author = {Sofia S Kudryavtseva and Igor A Yaroshevich and Aleksandra A Mamchur and Irina S Panina and Andrei V Moiseenko and Olga S. Sokolova and Vladimir I. Muronetz and Tatiana B Stanishneva Konovalova and Evgeny B Pichkur},
title = {Novel cryo-EM structure of an ADP-bound GroEL–GroES complex},
journal = {Scientific Reports},
year = {2021},
volume = {11},
publisher = {Springer Nature},
month = {sep},
url = {https://doi.org/10.1038%2Fs41598-021-97657-x},
number = {1},
doi = {10.1038/s41598-021-97657-x}
}
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