Novel cryo-EM structure of an ADP-bound GroEL–GroES complex
The GroEL–GroES chaperonin complex is a bacterial protein folding system, functioning in an ATP-dependent manner. Upon ATP binding and hydrolysis, it undergoes multiple stages linked to substrate protein binding, folding and release. Structural methods helped to reveal several conformational states and provide more information about the chaperonin functional cycle. Here, using cryo-EM we resolved two nucleotide-bound structures of the bullet-shaped GroEL–GroES1 complex at 3.4 Å resolution. The main difference between them is the relative orientation of their apical domains. Both structures contain nucleotides in cis and trans GroEL rings; in contrast to previously reported bullet-shaped complexes where nucleotides were only present in the cis ring. Our results suggest that the bound nucleotides correspond to ADP, and that such a state appears at low ATP:ADP ratios.
Citations by journals
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Biomedicines
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Biomedicines
1 publication, 11.11%
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Journal of Microbiology
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Journal of Microbiology, 1, 11.11%
Journal of Microbiology
1 publication, 11.11%
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Journal of Physical Chemistry Letters
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Journal of Physical Chemistry Letters
1 publication, 11.11%
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Toxins
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Toxins
1 publication, 11.11%
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Proceedings of the National Academy of Sciences of the United States of America
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Proceedings of the National Academy of Sciences of the United States of America
1 publication, 11.11%
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Advanced Science
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Advanced Science
1 publication, 11.11%
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1
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Citations by publishers
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2
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 22.22%
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Han-Gug Misaengmul Hag-hoe/The Microbiological Society of Korea
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Han-Gug Misaengmul Hag-hoe/The Microbiological Society of Korea, 1, 11.11%
Han-Gug Misaengmul Hag-hoe/The Microbiological Society of Korea
1 publication, 11.11%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
1 publication, 11.11%
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Proceedings of the National Academy of Sciences (PNAS)
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Proceedings of the National Academy of Sciences (PNAS)
1 publication, 11.11%
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Wiley
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Wiley
1 publication, 11.11%
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2
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