Open Access
Open access
Scientific Reports, volume 12, issue 1, publication number 17147

Assessment of variability in the plasma 7k SomaScan proteomics assay

Publication typeJournal Article
Publication date2022-10-13
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor4.6
ISSN20452322, 20452322
Multidisciplinary
Abstract
SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.

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GOST |
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GOST Copy
Candia J. et al. Assessment of variability in the plasma 7k SomaScan proteomics assay // Scientific Reports. 2022. Vol. 12. No. 1. 17147
GOST all authors (up to 50) Copy
Candia J., Daya G. N., Tanaka T., Ferrucci L., Walker K. A. Assessment of variability in the plasma 7k SomaScan proteomics assay // Scientific Reports. 2022. Vol. 12. No. 1. 17147
RIS |
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RIS Copy
TY - JOUR
DO - 10.1038/s41598-022-22116-0
UR - https://doi.org/10.1038/s41598-022-22116-0
TI - Assessment of variability in the plasma 7k SomaScan proteomics assay
T2 - Scientific Reports
AU - Candia, Julián
AU - Daya, Gulzar N
AU - Tanaka, Toshiko
AU - Ferrucci, Luigi
AU - Walker, Keenan A.
PY - 2022
DA - 2022/10/13 00:00:00
PB - Springer Nature
IS - 1
VL - 12
SN - 2045-2322
SN - 2045-2322
ER -
BibTex
Cite this
BibTex Copy
@article{2022_Candia,
author = {Julián Candia and Gulzar N Daya and Toshiko Tanaka and Luigi Ferrucci and Keenan A. Walker},
title = {Assessment of variability in the plasma 7k SomaScan proteomics assay},
journal = {Scientific Reports},
year = {2022},
volume = {12},
publisher = {Springer Nature},
month = {oct},
url = {https://doi.org/10.1038/s41598-022-22116-0},
number = {1},
doi = {10.1038/s41598-022-22116-0}
}
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