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Scientific Reports, volume 13, issue 1, publication number 8546

Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer

Publication type: Journal Article
Publication date: 2023-05-26
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 4.6
ISSN: 20452322
Multidisciplinary
Abstract

We report a new approach to controllable thermal stimulation of a single living cell and its compartments. The technique is based on the use of a single polycrystalline diamond particle containing silicon-vacancy (SiV) color centers. Due to the presence of amorphous carbon at its intercrystalline boundaries, such a particle is an efficient light absorber and becomes a local heat source when illuminated by a laser. Furthermore, the temperature of such a local heater is tracked by the spectral shift of the zero-phonon line of SiV centers. Thus, the diamond particle acts simultaneously as a heater and a thermometer. In the current work, we demonstrate the ability of such a Diamond Heater-Thermometer (DHT) to locally alter the temperature, one of the numerous parameters that play a decisive role for the living organisms at the nanoscale. In particular, we show that the local heating of 11–12 °C relative to the ambient temperature (22 °C) next to individual HeLa cells and neurons, isolated from the mouse hippocampus, leads to a change in the intracellular distribution of the concentration of free calcium ions. For individual HeLa cells, a long-term (about 30 s) increase in the integral intensity of Fluo-4 NW fluorescence by about three times is observed, which characterizes an increase in the [Ca2+]cyt concentration of free calcium in the cytoplasm. Heating near mouse hippocampal neurons also caused a calcium surge—an increase in the intensity of Fluo-4 NW fluorescence by 30% and a duration of ~ 0.4 ms.

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Nanomaterials
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Nanomaterials
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Carbon
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Carbon
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Multidisciplinary Digital Publishing Institute (MDPI)
Multidisciplinary Digital Publishing Institute (MDPI), 1, 50%
Multidisciplinary Digital Publishing Institute (MDPI)
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Elsevier
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Elsevier
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Romshin A. M. et al. Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer // Scientific Reports. 2023. Vol. 13. No. 1. 8546
GOST all authors (up to 50) Copy
Romshin A. M., Zeeb V., Glushkov E., Radenovic A., Sinogeikin A. G., Vlasov I. I. Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer // Scientific Reports. 2023. Vol. 13. No. 1. 8546
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TY - JOUR
DO - 10.1038/s41598-023-35141-4
UR - https://doi.org/10.1038%2Fs41598-023-35141-4
TI - Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer
T2 - Scientific Reports
AU - Romshin, Alexey M.
AU - Zeeb, Vadim
AU - Glushkov, Evgenii
AU - Radenovic, Aleksandra
AU - Sinogeikin, Andrey G.
AU - Vlasov, Igor I.
PY - 2023
DA - 2023/05/26 00:00:00
PB - Springer Nature
IS - 1
VL - 13
PMID - 37236978
SN - 2045-2322
ER -
BibTex
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BibTex Copy
@article{2023_Romshin,
author = {Alexey M. Romshin and Vadim Zeeb and Evgenii Glushkov and Aleksandra Radenovic and Andrey G. Sinogeikin and Igor I. Vlasov},
title = {Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer},
journal = {Scientific Reports},
year = {2023},
volume = {13},
publisher = {Springer Nature},
month = {may},
url = {https://doi.org/10.1038%2Fs41598-023-35141-4},
number = {1},
doi = {10.1038/s41598-023-35141-4}
}
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