Nature Reviews Methods Primers

, volume 4 , issue 1 , publication number 56

Stimulated emission depletion microscopy

Gražvydas Lukinavičius ¹

Jonatan Alvelid ^{2, 3}

Rūta Gerasimaitė ¹

Carmen Rodilla-Ramirez ⁴

Van Thang Nguyen ¹

Giuseppe Vicidomini ⁵

Francesca Bottanelli ⁴

Kyu Young Han ⁶

Ilaria Testa ³

Hide authors affiliations Show authors affiliations: 6 affiliations

Chromatin Labeling and Imaging group, Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany |

Department of Biophysical Imaging, Leibniz Institute of Photonic Technology, Jena, Germany |

Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden |

⁴

Institut für Biochemie, Freie Universität Berlin, Berlin, Germany |

⁵

Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy |

⁶

CREOL, The college of Optics and Photonics, University of Central Florida, Orlando, USA |

Publication type: Journal Article

Publication date: 2024-08-08

Springer Nature

Nature Reviews Methods Primers

scimago Q1

wos Q1

SJR: 15.026

CiteScore: 85.0

Impact factor: 56.0

ISSN: 26628449

DOI: 10.1038/s43586-024-00335-1

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Abstract

In this Primer, we focus on the most recent advancements in stimulated emission depletion (STED) microscopy, encompassing optics, computational microscopy and probes design, which enable STED imaging to open new observation windows in challenging samples such as living cells and tissues. We showcase applications in which STED data have been essential to gain new biological insights in various cell types and model systems. Finally, we discuss what standardization will be important in our view to further advance STED imaging, including open and shareable software, analysis pipelines, data repositories and sample preparation protocols. Stimulated emission depletion microscopy opens new observation windows in challenging samples such as living cells and tissues. In this Primer, Lukinavičius et al. discuss 2D and 3D stimulated emission depletion setup, including adaptive optical elements and their combination with fluorescence lifetime techniques.

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GOST Copy

Lukinavičius G. et al. Stimulated emission depletion microscopy // Nature Reviews Methods Primers. 2024. Vol. 4. No. 1. 56

GOST all authors (up to 50) Copy

Lukinavičius G., Alvelid J., Gerasimaitė R., Rodilla-Ramirez C., Nguyen V. T., Vicidomini G., Bottanelli F., Han K. Y., Testa I. Stimulated emission depletion microscopy // Nature Reviews Methods Primers. 2024. Vol. 4. No. 1. 56

RIS |

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RIS Copy

TY - JOUR

DO - 10.1038/s43586-024-00335-1

UR - https://www.nature.com/articles/s43586-024-00335-1

TI - Stimulated emission depletion microscopy

T2 - Nature Reviews Methods Primers

AU - Lukinavičius, Gražvydas

AU - Alvelid, Jonatan

AU - Gerasimaitė, Rūta

AU - Rodilla-Ramirez, Carmen

AU - Nguyen, Van Thang

AU - Vicidomini, Giuseppe

AU - Bottanelli, Francesca

AU - Han, Kyu Young

AU - Testa, Ilaria

PY - 2024

DA - 2024/08/08

PB - Springer Nature

IS - 1

VL - 4

SN - 2662-8449

ER -

BibTex

Cite this

BibTex (up to 50 authors) Copy

@article{2024_Lukinavičius,

author = {Gražvydas Lukinavičius and Jonatan Alvelid and Rūta Gerasimaitė and Carmen Rodilla-Ramirez and Van Thang Nguyen and Giuseppe Vicidomini and Francesca Bottanelli and Kyu Young Han and Ilaria Testa},

title = {Stimulated emission depletion microscopy},

journal = {Nature Reviews Methods Primers},

year = {2024},

volume = {4},

publisher = {Springer Nature},

month = {aug},

url = {https://www.nature.com/articles/s43586-024-00335-1},

number = {1},

pages = {56},

doi = {10.1038/s43586-024-00335-1}

}

Publisher

Springer Nature

Journal

Nature Reviews Methods Primers

scimago Q1

wos Q1

SJR

15.026

CiteScore

85.0

Impact factor

56.0

ISSN

26628449 (Electronic)

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