Open Access

Scientific Reports

, volume 7 , issue 1 , publication number 42771

Label-free Detection of Influenza Viruses using a Reduced Graphene Oxide-based Electrochemical Immunosensor Integrated with a Microfluidic Platform

Renu Singh ¹

Seongkyeol Hong ¹

Jaesung Jang ^{1, 2}

Hide authors affiliations Show authors affiliations: 2 affiliations

School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Republic of Korea |

Department of Biomedical Engineering, UNIST, Ulsan, Republic of Korea |

Publication type: Journal Article

Publication date: 2017-02-15

Springer Nature

Scientific Reports

scimago Q1

wos Q1

SJR: 0.874

CiteScore: 6.7

Impact factor: 3.9

ISSN: 20452322

DOI: 10.1038/srep42771

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PubMed ID: 28198459

Multidisciplinary

Abstract

Reduced graphene oxide (RGO) has recently gained considerable attention for use in electrochemical biosensing applications due to its outstanding conducting properties and large surface area. This report presents a novel microfluidic chip integrated with an RGO-based electrochemical immunosensor for label-free detection of an influenza virus, H1N1. Three microelectrodes were fabricated on a glass substrate using the photolithographic technique, and the working electrode was functionalized using RGO and monoclonal antibodies specific to the virus. These chips were integrated with polydimethylsiloxane microchannels. Structural and morphological characterizations were performed using X-ray photoelectron spectroscopy and scanning electron microscopy. Electrochemical studies revealed good selectivity and an enhanced detection limit of 0.5 PFU mL−1, where the chronoamperometric current increased linearly with H1N1 virus concentration within the range of 1 to 104 PFU mL−1 (R2 = 0.99). This microfluidic immunosensor can provide a promising platform for effective detection of biomolecules using minute samples.

Found

3 citations

Sengupta Joydip

🥼 🤝

66 publications, 1 079 citations, 79 reviews

h-index: 18

University of Calcutta

Research interests

Graphene

Zinc Oxide

1 citation

Prabhakar Pranav

🥼 🤝

PhD in Biological/biomedical sciences, professor, full member of the Indian National Science Academy

122 publications, 1 647 citations, 70 reviews

h-index: 20

Indian Institute of Technology Madras

Lovely Professional University

Nagaland University

Research interests

Diabetes

1 citation

Ivin Yury

PhD in Biological/biomedical sciences

33 publications, 243 citations

h-index: 7

M.P. Chumakov Federal Scientific Center for Research and Development of Immunobiological Drugs of the Russian Academy of Sciences

Research interests

Cell biology

Industrial biotechnology

Vaccine

Viruses

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Singh R. et al. Label-free Detection of Influenza Viruses using a Reduced Graphene Oxide-based Electrochemical Immunosensor Integrated with a Microfluidic Platform // Scientific Reports. 2017. Vol. 7. No. 1. 42771

GOST all authors (up to 50) Copy

Singh R., Hong S., Jang J. Label-free Detection of Influenza Viruses using a Reduced Graphene Oxide-based Electrochemical Immunosensor Integrated with a Microfluidic Platform // Scientific Reports. 2017. Vol. 7. No. 1. 42771

RIS |

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RIS Copy

TY - JOUR

DO - 10.1038/srep42771

UR - https://doi.org/10.1038/srep42771

TI - Label-free Detection of Influenza Viruses using a Reduced Graphene Oxide-based Electrochemical Immunosensor Integrated with a Microfluidic Platform

T2 - Scientific Reports

AU - Singh, Renu

AU - Hong, Seongkyeol

AU - Jang, Jaesung

PY - 2017

DA - 2017/02/15

PB - Springer Nature

IS - 1

VL - 7

PMID - 28198459

SN - 2045-2322

ER -

BibTex

Cite this

BibTex (up to 50 authors) Copy

@article{2017_Singh,

author = {Renu Singh and Seongkyeol Hong and Jaesung Jang},

title = {Label-free Detection of Influenza Viruses using a Reduced Graphene Oxide-based Electrochemical Immunosensor Integrated with a Microfluidic Platform},

journal = {Scientific Reports},

year = {2017},

volume = {7},

publisher = {Springer Nature},

month = {feb},

url = {https://doi.org/10.1038/srep42771},

number = {1},

pages = {42771},

doi = {10.1038/srep42771}

}

Publisher

Springer Nature

Journal

Scientific Reports

scimago Q1

wos Q1

SJR

0.874

CiteScore

6.7

Impact factor

3.9

ISSN

20452322 (Print, Electronic)