PELDOR analysis of enzyme-induced structural changes in damaged DNA duplexes
A D Milov
2
,
N P Isaev
2
,
Yu N Vorobjev
1
,
Vladimir Yu. Koval
1
,
Sergei A. Dzuba
2
,
О. С. Федорова
1
,
Yu.D. Tsvetkov
2
Publication type: Journal Article
Publication date: 2011-07-06
SJR: —
CiteScore: —
Impact factor: —
ISSN: 1742206X, 17422051
PubMed ID:
21731955
Molecular Biology
Biotechnology
Abstract
PELDOR (pulsed electron-electron double resonance) spectroscopy was applied to determine spin-spin distances in spin-labeled DNA duplexes (13-mer and 17-mer) containing the damaged sites 8-oxoguanine or uncleavable abasic site analogue tetrahydrofuran. The lesions were located in one strand of the DNA, and two nitroxyl spin labels were attached at the 5'- and 3'-ends of the complementary strand. PELDOR data allow us to obtain distances between the two spin labels in DNAs, which turned out to be around 5 nm for the 13-mer DNA and around 6 nm for 17-mer DNA. Results of PELDOR measurements were supported by molecular dynamics calculations. Study of the interaction of DNA fragments with DNA repair enzyme 8-oxoguanine-DNA glycosylase from E. coli (Fpg protein) showed that this interaction leads to a noticeable decrease of the distance between spin labels, which indicates the enzyme-induced bending of the DNA duplex. This bending may be important for the mechanisms of recognition of damaged sites by DNA repair enzymes.
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GOST
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Kuznetsov N. A. et al. PELDOR analysis of enzyme-induced structural changes in damaged DNA duplexes // Molecular BioSystems. 2011. Vol. 7. No. 9. p. 2670.
GOST all authors (up to 50)
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Kuznetsov N. A., Milov A. D., Isaev N. P., Vorobjev Yu. N., Koval V. Y., Dzuba S. A., Федорова О. С., Tsvetkov Y. PELDOR analysis of enzyme-induced structural changes in damaged DNA duplexes // Molecular BioSystems. 2011. Vol. 7. No. 9. p. 2670.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1039/c1mb05189j
UR - https://doi.org/10.1039/c1mb05189j
TI - PELDOR analysis of enzyme-induced structural changes in damaged DNA duplexes
T2 - Molecular BioSystems
AU - Kuznetsov, Nikita A.
AU - Milov, A D
AU - Isaev, N P
AU - Vorobjev, Yu N
AU - Koval, Vladimir Yu.
AU - Dzuba, Sergei A.
AU - Федорова, О. С.
AU - Tsvetkov, Yu.D.
PY - 2011
DA - 2011/07/06
PB - Royal Society of Chemistry (RSC)
SP - 2670
IS - 9
VL - 7
PMID - 21731955
SN - 1742-206X
SN - 1742-2051
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2011_Kuznetsov,
author = {Nikita A. Kuznetsov and A D Milov and N P Isaev and Yu N Vorobjev and Vladimir Yu. Koval and Sergei A. Dzuba and О. С. Федорова and Yu.D. Tsvetkov},
title = {PELDOR analysis of enzyme-induced structural changes in damaged DNA duplexes},
journal = {Molecular BioSystems},
year = {2011},
volume = {7},
publisher = {Royal Society of Chemistry (RSC)},
month = {jul},
url = {https://doi.org/10.1039/c1mb05189j},
number = {9},
pages = {2670},
doi = {10.1039/c1mb05189j}
}
Cite this
MLA
Copy
Kuznetsov, Nikita A., et al. “PELDOR analysis of enzyme-induced structural changes in damaged DNA duplexes.” Molecular BioSystems, vol. 7, no. 9, Jul. 2011, p. 2670. https://doi.org/10.1039/c1mb05189j.
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