In situ microfluidic SERS assay for monitoring enzymatic breakdown of organophosphates
Vladimir Liberman
1, 2, 3, 4, 5
,
Kimberly Hamad-Schifferli
1, 5, 6
,
Todd A. Thorsen
1, 2, 3, 4, 5
,
Scott Wick
1, 5, 6
,
Peter A. Carr
1, 5, 6
1
MIT Lincoln Laboratory
2
Chemical
3
Microsystem
4
and Nanoscale Technologies
5
Lexington, USA
|
6
Bioengineering Systems and Technologies
Publication type: Journal Article
Publication date: 2015-06-03
scimago Q1
wos Q1
SJR: 1.245
CiteScore: 9.9
Impact factor: 5.1
ISSN: 20403364, 20403372
PubMed ID:
26041657
General Materials Science
Abstract
In this paper, we report on a method to probe the breakdown of the organophosphate (OP) simulants o,s-diethyl methyl phosphonothioate (OSDMP) and demeton S by the enzyme organophosphorous hydrolase (OPH) in a microfluidic device by surface enhanced Raman spectroscopy (SERS). SERS hotspots were formed on-demand inside the microfluidic device by laser-induced aggregation of injected Ag NPs suspensions. The Ag NP clusters, covering micron-sized areas, were formed within minutes using a conventional confocal Raman laser microscope. These Ag NP clusters were used to enhance the Raman spectra of the thiol products of OP breakdown in the microfluidic device: ethanethiol (EtSH) and (ethylsulfanyl) ethane-1-thiol (2-EET). When the OPH enzyme and its substrates OSDMP and demeton S were introduced, the thiolated breakdown products were generated, resulting in changes in the SERS spectra. With the ability to analyze reaction volumes as low as 20 nL, our approach demonstrates great potential for miniaturization of SERS analytical protocols.
Found
Nothing found, try to update filter.
Found
Nothing found, try to update filter.
Top-30
Journals
|
1
|
|
|
Microsystem Technologies
1 publication, 11.11%
|
|
|
Optics and Laser Technology
1 publication, 11.11%
|
|
|
Sensors and Actuators, B: Chemical
1 publication, 11.11%
|
|
|
Coordination Chemistry Reviews
1 publication, 11.11%
|
|
|
Electrophoresis
1 publication, 11.11%
|
|
|
Journal of Separation Science
1 publication, 11.11%
|
|
|
ACS Synthetic Biology
1 publication, 11.11%
|
|
|
The Analyst
1 publication, 11.11%
|
|
|
Russian Chemical Reviews
1 publication, 11.11%
|
|
|
1
|
Publishers
|
1
2
3
|
|
|
Elsevier
3 publications, 33.33%
|
|
|
Wiley
2 publications, 22.22%
|
|
|
Springer Nature
1 publication, 11.11%
|
|
|
American Chemical Society (ACS)
1 publication, 11.11%
|
|
|
Royal Society of Chemistry (RSC)
1 publication, 11.11%
|
|
|
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 11.11%
|
|
|
1
2
3
|
- We do not take into account publications without a DOI.
- Statistics recalculated weekly.
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
9
Total citations:
9
Citations from 2024:
1
(11.11%)
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Liberman V. et al. In situ microfluidic SERS assay for monitoring enzymatic breakdown of organophosphates // Nanoscale. 2015. Vol. 7. No. 25. pp. 11013-11023.
GOST all authors (up to 50)
Copy
Liberman V., Hamad-Schifferli K., Thorsen T. A., Wick S., Carr P. A. In situ microfluidic SERS assay for monitoring enzymatic breakdown of organophosphates // Nanoscale. 2015. Vol. 7. No. 25. pp. 11013-11023.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1039/c5nr01974e
UR - https://doi.org/10.1039/c5nr01974e
TI - In situ microfluidic SERS assay for monitoring enzymatic breakdown of organophosphates
T2 - Nanoscale
AU - Liberman, Vladimir
AU - Hamad-Schifferli, Kimberly
AU - Thorsen, Todd A.
AU - Wick, Scott
AU - Carr, Peter A.
PY - 2015
DA - 2015/06/03
PB - Royal Society of Chemistry (RSC)
SP - 11013-11023
IS - 25
VL - 7
PMID - 26041657
SN - 2040-3364
SN - 2040-3372
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2015_Liberman,
author = {Vladimir Liberman and Kimberly Hamad-Schifferli and Todd A. Thorsen and Scott Wick and Peter A. Carr},
title = {In situ microfluidic SERS assay for monitoring enzymatic breakdown of organophosphates},
journal = {Nanoscale},
year = {2015},
volume = {7},
publisher = {Royal Society of Chemistry (RSC)},
month = {jun},
url = {https://doi.org/10.1039/c5nr01974e},
number = {25},
pages = {11013--11023},
doi = {10.1039/c5nr01974e}
}
Cite this
MLA
Copy
Liberman, Vladimir, et al. “In situ microfluidic SERS assay for monitoring enzymatic breakdown of organophosphates.” Nanoscale, vol. 7, no. 25, Jun. 2015, pp. 11013-11023. https://doi.org/10.1039/c5nr01974e.