Layered polymeric capsules inhibiting the activity of RNases for intracellular delivery of messenger RNA
Mitali Kakran
1
,
Masafumi Muratani
2
,
Weiquan John Tng
2
,
Hongqing Liang
2
,
Daria Trushina
1, 3
,
Gleb B. Sukhorukov
1, 4
,
HUCK-HUI NG
2
,
Huck Hui Ng
2
,
1
2
3
Publication type: Journal Article
Publication date: 2015-06-16
scimago Q1
wos Q2
SJR: 1.159
CiteScore: 10.4
Impact factor: 5.7
ISSN: 20507518, 2050750X
PubMed ID:
32262581
General Chemistry
General Medicine
General Materials Science
Biomedical Engineering
Abstract
Intracellular delivery of messenger RNA (mRNA) is a promising approach for experimental and therapeutic manipulation of cellular activity. However, environmental RNase hinders reliable handling of mRNA for experimental and therapeutic use. In this study, biodegradable capsules composed of dextran sulfate and poly-l-arginine in the layer-by-layer (LbL) fashion are employed for the protection and delivery of mRNA. Our results demonstrate that addition of RNase inhibitors to mRNA while co-precipitation with CaCO3 and subsequent LbL encapsulation are both crucial to preserve the integrity of mRNA. The expression of functional luciferase enzyme in HEK293T human embryonic kidney cells after incubation with synthetic luciferase-encoding mRNA capsules indicates the reliability of the encapsulating system and cellular intake of functional mRNAs. These improvements in mRNA encapsulation should provide essential basis for microcapsule-based mRNA delivery for further applications.
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38
Total citations:
38
Citations from 2024:
3
(7%)
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GOST
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Kakran M. et al. Layered polymeric capsules inhibiting the activity of RNases for intracellular delivery of messenger RNA // Journal of Materials Chemistry B. 2015. Vol. 3. No. 28. pp. 5842-5848.
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Kakran M., Muratani M., Tng W. J., Liang H., Trushina D., Sukhorukov G. B., NG H., Ng H. H., Antipina M. N. Layered polymeric capsules inhibiting the activity of RNases for intracellular delivery of messenger RNA // Journal of Materials Chemistry B. 2015. Vol. 3. No. 28. pp. 5842-5848.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1039/c5tb00615e
UR - https://doi.org/10.1039/c5tb00615e
TI - Layered polymeric capsules inhibiting the activity of RNases for intracellular delivery of messenger RNA
T2 - Journal of Materials Chemistry B
AU - Kakran, Mitali
AU - Muratani, Masafumi
AU - Tng, Weiquan John
AU - Liang, Hongqing
AU - Trushina, Daria
AU - Sukhorukov, Gleb B.
AU - NG, HUCK-HUI
AU - Ng, Huck Hui
AU - Antipina, Maria N.
PY - 2015
DA - 2015/06/16
PB - Royal Society of Chemistry (RSC)
SP - 5842-5848
IS - 28
VL - 3
PMID - 32262581
SN - 2050-7518
SN - 2050-750X
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2015_Kakran,
author = {Mitali Kakran and Masafumi Muratani and Weiquan John Tng and Hongqing Liang and Daria Trushina and Gleb B. Sukhorukov and HUCK-HUI NG and Huck Hui Ng and Maria N. Antipina},
title = {Layered polymeric capsules inhibiting the activity of RNases for intracellular delivery of messenger RNA},
journal = {Journal of Materials Chemistry B},
year = {2015},
volume = {3},
publisher = {Royal Society of Chemistry (RSC)},
month = {jun},
url = {https://doi.org/10.1039/c5tb00615e},
number = {28},
pages = {5842--5848},
doi = {10.1039/c5tb00615e}
}
Cite this
MLA
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Kakran, Mitali, et al. “Layered polymeric capsules inhibiting the activity of RNases for intracellular delivery of messenger RNA.” Journal of Materials Chemistry B, vol. 3, no. 28, Jun. 2015, pp. 5842-5848. https://doi.org/10.1039/c5tb00615e.