A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types
Yana V Tarakanchikova
1, 2, 3, 4, 5, 6, 7, 8, 9
,
Albert Muslimov
2, 4, 9, 10, 11, 12, 13
,
IGOR SERGEEV
12, 14, 15, 16, 17
,
K. Lepik
4, 18
,
Kirill Lepik
11, 12, 13, 19, 20
,
Nikita Yolshin
12, 21, 22
,
Alexander Goncharenko
2, 4, 9, 10, 11, 12, 13
,
Kirill Vasilyev
12, 21, 22
,
Igor E. Eliseev
2, 9, 10, 11, 12
,
Anton Bukatin
2, 9, 10, 11, 12
,
Vladislav Sergeev
11, 12, 18, 20
,
Sergey Pavlov
12, 19, 23, 24
,
Alexey Popov
1, 25
,
A.P. Popov
1, 25
,
Igor Meglinski
1
,
Boris Afanasiev
11, 12, 18, 20
,
Bogdan V Parakhonskiy
26, 27, 28, 29, 30
,
Gleb B. Sukhorukov
3, 12, 19, 31, 32, 33, 34, 35
,
Dmitry A. Gorin
14, 32
,
Dmitry Gorin
12, 15, 16, 17, 36
4
“QR.bio” limited liability company, St. Petersburg, Russia
|
5
Opto-Electronics and Measurement Techniques Research Unit
7
Oulu
|
8
FINLAND
|
9
Nanobiotechnology Laboratory
11
Saint-Petersburg
|
12
Russia
|
13
“QR.bio” limited liability company
15
11 Center of Photonics & Quantum Materials
17
Moscow
19
St. Petersburg
27
Faculty of Bioscience Engineering
29
Ghent
|
30
BELGIUM
|
34
School of Engineering and Materials Science
36
Educational Research Institute of Nanostructures and Biosystems
Publication type: Journal Article
Publication date: 2020-09-07
scimago Q1
wos Q2
SJR: 1.159
CiteScore: 10.4
Impact factor: 5.7
ISSN: 20507518, 2050750X
PubMed ID:
33005912
General Chemistry
General Medicine
General Materials Science
Biomedical Engineering
Abstract
While DNA and messenger RNA (mRNA) based therapies are currently changing the biomedical field, the delivery of genetic materials remains the key problem preventing the wide introduction of these methods into clinical practice. Therefore, the creation of new methods for intracellular gene delivery, particularly to hard-to-transfect, clinically relevant cell populations is a pressing issue. Here, we report on the design of a novel approach to format 50-150 nm calcium carbonate particles in the vaterite state and using them as a template for polymeric core-shell nanoparticles. We apply such core-shell nanoparticles as safe and efficient carriers for mRNA and pDNA. We prove that such nanocarriers are actively internalized by up to 99% of primary T-lymphocytes and exert minimal toxicity with the viability of >90%. We demonstrate that these nanocarriers mediate more efficient transfection compared with the standard electroporation method (90% vs. 51% for mRNA and 62% vs. 39% for plasmid DNA) in primary human T-lymphocytes as a model of the hard to transfect type that is widely used in gene and cell therapy approaches. Importantly, these polymeric nanocarriers can be used in serum containing basic culture medium without special conditions and equipment, thus having potential for being introduced in clinical development. As a result, we have provided proof-of-principle that our nanosized containers represent a promising universal non-viral platform for efficient and safe gene delivery.
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Total citations:
27
Citations from 2024:
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(18.52%)
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MLA
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GOST
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Tarakanchikova Y. V. et al. A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types // Journal of Materials Chemistry B. 2020. Vol. 8. No. 41. pp. 9576-9588.
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Tarakanchikova Y. V. et al. A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types // Journal of Materials Chemistry B. 2020. Vol. 8. No. 41. pp. 9576-9588.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1039/d0tb01359e
UR - https://xlink.rsc.org/?DOI=D0TB01359E
TI - A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types
T2 - Journal of Materials Chemistry B
AU - Tarakanchikova, Yana V
AU - Muslimov, Albert
AU - SERGEEV, IGOR
AU - Lepik, K.
AU - Lepik, Kirill
AU - Yolshin, Nikita
AU - Goncharenko, Alexander
AU - Vasilyev, Kirill
AU - Eliseev, Igor E.
AU - Bukatin, Anton
AU - Sergeev, Vladislav
AU - Pavlov, Sergey
AU - Popov, Alexey
AU - Popov, A.P.
AU - Meglinski, Igor
AU - Afanasiev, Boris
AU - Parakhonskiy, Bogdan V
AU - Sukhorukov, Gleb B.
AU - Gorin, Dmitry A.
AU - Gorin, Dmitry
PY - 2020
DA - 2020/09/07
PB - Royal Society of Chemistry (RSC)
SP - 9576-9588
IS - 41
VL - 8
PMID - 33005912
SN - 2050-7518
SN - 2050-750X
ER -
Cite this
BibTex (up to 50 authors)
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@article{2020_Tarakanchikova,
author = {Yana V Tarakanchikova and Albert Muslimov and IGOR SERGEEV and K. Lepik and Kirill Lepik and Nikita Yolshin and Alexander Goncharenko and Kirill Vasilyev and Igor E. Eliseev and Anton Bukatin and Vladislav Sergeev and Sergey Pavlov and Alexey Popov and A.P. Popov and Igor Meglinski and Boris Afanasiev and Bogdan V Parakhonskiy and Gleb B. Sukhorukov and Dmitry A. Gorin and Dmitry Gorin and others},
title = {A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types},
journal = {Journal of Materials Chemistry B},
year = {2020},
volume = {8},
publisher = {Royal Society of Chemistry (RSC)},
month = {sep},
url = {https://xlink.rsc.org/?DOI=D0TB01359E},
number = {41},
pages = {9576--9588},
doi = {10.1039/d0tb01359e}
}
Cite this
MLA
Copy
Tarakanchikova, Yana V., et al. “A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types.” Journal of Materials Chemistry B, vol. 8, no. 41, Sep. 2020, pp. 9576-9588. https://xlink.rsc.org/?DOI=D0TB01359E.