Review of Scientific Instruments, volume 86, issue 3, pages 33701

Open-frame system for single-molecule microscopy

Adriel K J Arsenault 1
Jason S Leith 1
Gil Henkin 1
Christopher M J Mcfaul 1
Matthew Tarling 1
Richard Talbot 1
Daniel Berard 1
Francois Michaud 1
Shane Scott 1
Sabrina R. Leslie 1
Show full list: 10 авторов
Publication typeJournal Article
Publication date2015-03-01
scimago Q2
wos Q3
SJR0.481
CiteScore2.9
Impact factor1.7
ISSN00346748, 10897623
PubMed ID:  25832232
Instrumentation
Abstract

We present the design and construction of a versatile, open frame inverted microscope system for wide-field fluorescence and single molecule imaging. The microscope chassis and modular design allow for customization, expansion, and experimental flexibility. We present two components which are included with the microscope which extend its basic capabilities and together create a powerful microscopy system: A Convex Lens-induced Confinement device provides the system with single-molecule imaging capabilities, and a two-color imaging system provides the option of imaging multiple molecular species simultaneously. The flexibility of the open-framed chassis combined with accessible single-molecule, multi-species imaging technology supports a wide range of new measurements in the health, nanotechnology, and materials science research sectors.

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