,
volume 2064
Application of fluorescence and scanning electron microscopy for the investigation of cell contact guidance
Publication type: Proceedings Article
Publication date: 2019-01-16
SJR: 0.153
CiteScore: 0.5
Impact factor: —
ISSN: 0094243X, 15517616
Abstract
Cell contact guidance is the phenomenon of changes in cell shape, adhesion and migration in response to the substrate anisotropy. Here, we describe the application of fluorescence and scanning electron microscopy for the investigation of contact guidance. The model system used in this study consisted of HaCaT human keratinocytes grown on either random or aligned electrospun mats. Scanning electron microscopy allowed us to describe the cell shape and visualize both the nanofibers and the cell surface. The fluorescence microscopy was used to assess the influence of the substrate morphology on cell proliferation. HaCaT cells proliferated more intensely on the aligned mats than on the random ones.
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Sokolova A. I. et al. Application of fluorescence and scanning electron microscopy for the investigation of cell contact guidance // AIP Conference Proceedings. 2019. Vol. 2064.
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Sokolova A. I., Pavlova E. R., Bagrov D. V., Klinov D. V., Shaitan K. V., Khramova Yu. V. Application of fluorescence and scanning electron microscopy for the investigation of cell contact guidance // AIP Conference Proceedings. 2019. Vol. 2064.
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TY - CPAPER
DO - 10.1063/1.5087660
UR - https://doi.org/10.1063/1.5087660
TI - Application of fluorescence and scanning electron microscopy for the investigation of cell contact guidance
T2 - AIP Conference Proceedings
AU - Sokolova, A. I.
AU - Pavlova, E R
AU - Bagrov, D V
AU - Klinov, D. V.
AU - Shaitan, K V
AU - Khramova, Yu V
PY - 2019
DA - 2019/01/16
PB - AIP Publishing
VL - 2064
SN - 0094-243X
SN - 1551-7616
ER -
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@inproceedings{2019_Sokolova,
author = {A. I. Sokolova and E R Pavlova and D V Bagrov and D. V. Klinov and K V Shaitan and Yu V Khramova},
title = {Application of fluorescence and scanning electron microscopy for the investigation of cell contact guidance},
year = {2019},
volume = {2064},
month = {jan},
publisher = {AIP Publishing}
}