Open Access
Open access

Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome

Nelson B Olivier 1
Roger B Altman 2
Jonas Noeske 3
Gregory S Basarab 4
Erin Code 1
Andrew D. Ferguson 1
Ning Gao 1
Jian Huang 1
Manuel F Juette 2
Stephania Livchak 1
Matthew D. Miller 4
D.Bryan Prince 1
Jamie H. D. Cate 3
Ed T Buurman 5
Scott C Blanchard 2
Publication typeJournal Article
Publication date2014-11-03
scimago Q1
wos Q1
SJR3.414
CiteScore16.5
Impact factor9.1
ISSN00278424, 10916490
Multidisciplinary
Abstract
Significance

The identification of negamycin’s binding site within helix 34 of the small subunit head domain and the elucidation of its mechanism of action during messenger RNA decoding provide a physical framework for exploring structure–activity relationships of this largely unexplored antibiotic class. These findings lay the foundation for the rational design of improved negamycin analogs that may one day serve as potent antibacterial agents in the clinic.

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GOST |
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GOST Copy
Olivier N. B. et al. Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome // Proceedings of the National Academy of Sciences of the United States of America. 2014. Vol. 111. No. 46. pp. 16274-16279.
GOST all authors (up to 50) Copy
Olivier N. B., Altman R. B., Noeske J., Basarab G. S., Code E., Ferguson A. D., Gao N., Huang J., Juette M. F., Livchak S., Miller M. D., Prince D., Cate J. H. D., Buurman E. T., Blanchard S. C. Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome // Proceedings of the National Academy of Sciences of the United States of America. 2014. Vol. 111. No. 46. pp. 16274-16279.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1073/pnas.1414401111
UR - https://doi.org/10.1073/pnas.1414401111
TI - Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome
T2 - Proceedings of the National Academy of Sciences of the United States of America
AU - Olivier, Nelson B
AU - Altman, Roger B
AU - Noeske, Jonas
AU - Basarab, Gregory S
AU - Code, Erin
AU - Ferguson, Andrew D.
AU - Gao, Ning
AU - Huang, Jian
AU - Juette, Manuel F
AU - Livchak, Stephania
AU - Miller, Matthew D.
AU - Prince, D.Bryan
AU - Cate, Jamie H. D.
AU - Buurman, Ed T
AU - Blanchard, Scott C
PY - 2014
DA - 2014/11/03
PB - Proceedings of the National Academy of Sciences (PNAS)
SP - 16274-16279
IS - 46
VL - 111
PMID - 25368144
SN - 0027-8424
SN - 1091-6490
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2014_Olivier,
author = {Nelson B Olivier and Roger B Altman and Jonas Noeske and Gregory S Basarab and Erin Code and Andrew D. Ferguson and Ning Gao and Jian Huang and Manuel F Juette and Stephania Livchak and Matthew D. Miller and D.Bryan Prince and Jamie H. D. Cate and Ed T Buurman and Scott C Blanchard},
title = {Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
year = {2014},
volume = {111},
publisher = {Proceedings of the National Academy of Sciences (PNAS)},
month = {nov},
url = {https://doi.org/10.1073/pnas.1414401111},
number = {46},
pages = {16274--16279},
doi = {10.1073/pnas.1414401111}
}
MLA
Cite this
MLA Copy
Olivier, Nelson B., et al. “Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome.” Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 46, Nov. 2014, pp. 16274-16279. https://doi.org/10.1073/pnas.1414401111.