Open Access
Proceedings of the National Academy of Sciences of the United States of America, volume 94, issue 21, pages 11190-11195
Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity
1
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215; and Department of Molecular and Human Genetics and Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, TX 77030
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Publication type: Journal Article
Publication date: 1997-10-14
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 11.1
ISSN: 00278424, 10916490
PubMed ID:
9326584
Multidisciplinary
Abstract
Telomerase is a specialized reverse transcriptase consisting of both RNA and protein components. Previous characterization of yeast telomerase function in vivo identified four EST (for ever shorter telomeres) genes that, when mutated, result in the phenotypes expected for a defect in telomerase. Consistent with this genetic prediction, the EST2 gene has recently been shown to encode the catalytic component of telomerase. Using an in vitro assay, we show here that telomerase activity is present in extracts prepared from yeast strains carrying est1-Delta, est3-Delta, and cdc13-2(est) mutations. Therefore, while these three genes are necessary for telomerase function in vivo, they do not encode components essential for core catalytic activity. When Est2p, the one EST gene product found to be essential for catalytic activity, was immunoprecipitated from extracts, the telomerase RNA subunit was also specifically precipitated, supporting the conclusion that these two components are in a stable complex.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Lingner J. et al. Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity // Proceedings of the National Academy of Sciences of the United States of America. 1997. Vol. 94. No. 21. pp. 11190-11195.
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Lingner J., Cech T. R., Hughes T. R., Lundblad V. Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity // Proceedings of the National Academy of Sciences of the United States of America. 1997. Vol. 94. No. 21. pp. 11190-11195.
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TY - JOUR
DO - 10.1073/pnas.94.21.11190
UR - https://doi.org/10.1073%2Fpnas.94.21.11190
TI - Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity
T2 - Proceedings of the National Academy of Sciences of the United States of America
AU - Lingner, Joachim
AU - Cech, Thomas R
AU - Hughes, Timothy R.
AU - Lundblad, Victoria
PY - 1997
DA - 1997/10/14 00:00:00
PB - Proceedings of the National Academy of Sciences (PNAS)
SP - 11190-11195
IS - 21
VL - 94
PMID - 9326584
SN - 0027-8424
SN - 1091-6490
ER -
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@article{1997_Lingner,
author = {Joachim Lingner and Thomas R Cech and Timothy R. Hughes and Victoria Lundblad},
title = {Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
year = {1997},
volume = {94},
publisher = {Proceedings of the National Academy of Sciences (PNAS)},
month = {oct},
url = {https://doi.org/10.1073%2Fpnas.94.21.11190},
number = {21},
pages = {11190--11195},
doi = {10.1073/pnas.94.21.11190}
}
Cite this
MLA
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Lingner, Joachim, et al. “Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity.” Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 21, Oct. 1997, pp. 11190-11195. https://doi.org/10.1073%2Fpnas.94.21.11190.