Open Access
Molecular and Cellular Proteomics, volume 13, issue 11, pages 2986-3000
Proteomic Analysis of the Human Cyclin-dependent Kinase Family Reveals a Novel CDK5 Complex Involved in Cell Growth and Migration
Xu Shuangbing
,
Li Xu
1
,
Gong Zihua
1
,
Wang Wenqi
1
,
Li Yujing
1
,
Nair Binoj Chandrasekharan
1
,
Piao Hailong
1
,
YANG KUNYU
2
,
Wada Hideo
2
,
1
Department of Experimental Radiation Oncology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030
|
Publication type: Journal Article
Publication date: 2014-11-01
Journal:
Molecular and Cellular Proteomics
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 7
ISSN: 15359476, 15359484
PubMed ID:
25096995
Biochemistry
Molecular Biology
Analytical Chemistry
Abstract
Cyclin-dependent kinases (CDKs) are the catalytic subunits of a family of mammalian heterodimeric serine/threonine kinases that play critical roles in the control of cell-cycle progression, transcription, and neuronal functions. However, the functions, substrates, and regulation of many CDKs are poorly understood. To systematically investigate these features of CDKs, we conducted a proteomic analysis of the CDK family and identified their associated protein complexes in two different cell lines using a modified SAINT (Significance Analysis of INTeractome) method. The mass spectrometry data were deposited to ProteomeXchange with identifier PXD000593 and DOI 10.6019/PXD000593. We identified 753 high-confidence candidate interaction proteins (HCIPs) in HEK293T cells and 352 HCIPs in MCF10A cells. We subsequently focused on a neuron-specific CDK, CDK5, and uncovered two novel CDK5-binding partners, KIAA0528 and fibroblast growth factor (acidic) intracellular binding protein (FIBP), in non-neuronal cells. We showed that these three proteins form a stable complex, with KIAA0528 and FIBP being required for the assembly and stability of the complex. Furthermore, CDK5-, KIAA0528-, or FIBP-depleted breast cancer cells displayed impaired proliferation and decreased migration, suggesting that this complex is required for cell growth and migration in non-neural cells. Our study uncovers new aspects of CDK functions, which provide direction for further investigation of these critical protein kinases.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Xu S. et al. Proteomic Analysis of the Human Cyclin-dependent Kinase Family Reveals a Novel CDK5 Complex Involved in Cell Growth and Migration // Molecular and Cellular Proteomics. 2014. Vol. 13. No. 11. pp. 2986-3000.
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Xu S., Li X., Gong Z., Wang W., Li Y., Nair B. C., Piao H., YANG K., Wada H., Junjie Chen J. C. Proteomic Analysis of the Human Cyclin-dependent Kinase Family Reveals a Novel CDK5 Complex Involved in Cell Growth and Migration // Molecular and Cellular Proteomics. 2014. Vol. 13. No. 11. pp. 2986-3000.
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TY - JOUR
DO - 10.1074/mcp.M113.036699
UR - https://doi.org/10.1074%2Fmcp.M113.036699
TI - Proteomic Analysis of the Human Cyclin-dependent Kinase Family Reveals a Novel CDK5 Complex Involved in Cell Growth and Migration
T2 - Molecular and Cellular Proteomics
AU - Xu, Shuangbing
AU - Li, Xu
AU - Gong, Zihua
AU - Wang, Wenqi
AU - Li, Yujing
AU - Nair, Binoj Chandrasekharan
AU - Piao, Hailong
AU - YANG, KUNYU
AU - Wada, Hideo
AU - Junjie Chen, Junjie Chen
PY - 2014
DA - 2014/11/01 00:00:00
PB - American Society for Biochemistry and Molecular Biology
SP - 2986-3000
IS - 11
VL - 13
PMID - 25096995
SN - 1535-9476
SN - 1535-9484
ER -
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@article{2014_Xu,
author = {Shuangbing Xu and Xu Li and Zihua Gong and Wenqi Wang and Yujing Li and Binoj Chandrasekharan Nair and Hailong Piao and KUNYU YANG and Hideo Wada and Junjie Chen Junjie Chen},
title = {Proteomic Analysis of the Human Cyclin-dependent Kinase Family Reveals a Novel CDK5 Complex Involved in Cell Growth and Migration},
journal = {Molecular and Cellular Proteomics},
year = {2014},
volume = {13},
publisher = {American Society for Biochemistry and Molecular Biology},
month = {nov},
url = {https://doi.org/10.1074%2Fmcp.M113.036699},
number = {11},
pages = {2986--3000},
doi = {10.1074/mcp.M113.036699}
}
Cite this
MLA
Copy
Xu, Shuangbing, et al. “Proteomic Analysis of the Human Cyclin-dependent Kinase Family Reveals a Novel CDK5 Complex Involved in Cell Growth and Migration.” Molecular and Cellular Proteomics, vol. 13, no. 11, Nov. 2014, pp. 2986-3000. https://doi.org/10.1074%2Fmcp.M113.036699.