Open Access
Virulence, volume 10, issue 1, pages 999-1012
Metarhizium brunneum infection dynamics differ at the cuticle interface of susceptible and tolerant morphs of Galleria mellonella
Publication type: Journal Article
Publication date: 2019-01-01
PubMed ID:
31724467
Microbiology (medical)
Microbiology
Infectious Diseases
Immunology
Parasitology
Abstract
ABSTRACT In order for entomopathogenic fungi to colonize an insect host, they must first attach to, and penetrate, the cuticle layers of the integument. Herein, we explored the interactions between the fungal pathogen Metarhizium brunneum ARSEF 4556 and two immunologically distinct morphs, melanic (M) and non-melanic (NM), of the greater wax moth Galleria mellonella. We first interrogated the cuticular compositions of both insect morphs to reveal substantial differences in their physiochemical properties. Enhanced melanin accumulation, fewer hydrocarbons, and higher L-dihydroxyphenylalanine (DOPA) decarboxylase activity were evident in the cuticle of the M larvae. This “hostile” terrain proved challenging for M. brunneum – reflected in poor conidial attachment and germination, and elevated expression of stress-associated genes (e.g., Hsp30, Hsp70). Lack of adherence to the cuticle impacted negatively on the speed of kill and overall host mortality; a dose of 107 conidia killed ~30% of M larvae over a 12-day period, whereas a 100-fold lower dose (105 conidia) achieved a similar result for NM larvae. Candidate gene expression patterns between the insect morphs indicated that M larvae are primed to “switch-on” immunity-associated genes (e.g., phenoloxidase) within 6–12 h of conidia exposure and can sustain a “defense” response. Critically, M. brunneum responds to the distinct physiochemical cues of both hosts and adjusts the expression of pathogenicity-related genes accordingly (e.g., Pr2, Mad1, Mad2). We reveal previously uncharacterized mechanisms of attack and defence in fungal-insect antibiosis.
Citations by journals
|
1
2
|
|
|
Journal of Fungi
|
Journal of Fungi
2 publications, 10.53%
|
|
Journal of Invertebrate Pathology
|
Journal of Invertebrate Pathology
2 publications, 10.53%
|
|
Virulence
|
Virulence
2 publications, 10.53%
|
|
Microbial Pathogenesis
|
Microbial Pathogenesis
1 publication, 5.26%
|
|
Pathogens
|
Pathogens
1 publication, 5.26%
|
|
Archives of Toxicology
|
Archives of Toxicology
1 publication, 5.26%
|
|
Parasites and Vectors
|
Parasites and Vectors
1 publication, 5.26%
|
|
ISME Journal
|
ISME Journal
1 publication, 5.26%
|
|
Folia Microbiologica
|
Folia Microbiologica
1 publication, 5.26%
|
|
Biological Control
|
Biological Control
1 publication, 5.26%
|
|
Developmental and Comparative Immunology
|
Developmental and Comparative Immunology
1 publication, 5.26%
|
|
Food and Chemical Toxicology
|
Food and Chemical Toxicology
1 publication, 5.26%
|
|
Entomologia Experimentalis et Applicata
|
Entomologia Experimentalis et Applicata
1 publication, 5.26%
|
|
Critical Reviews in Biotechnology
|
Critical Reviews in Biotechnology
1 publication, 5.26%
|
|
Fungal Biology
|
Fungal Biology
1 publication, 5.26%
|
|
1
2
|
Citations by publishers
|
1
2
3
4
5
6
7
|
|
|
Elsevier
|
Elsevier
7 publications, 36.84%
|
|
Springer Nature
|
Springer Nature
4 publications, 21.05%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 15.79%
|
|
Taylor & Francis
|
Taylor & Francis
3 publications, 15.79%
|
|
Wiley
|
Wiley
1 publication, 5.26%
|
|
1
2
3
4
5
6
7
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023,2024],"ids":[0,0,0,0,0],"codes":[0,0,0,0,0],"imageUrls":["","","","",""],"datasets":[{"label":"Citations number","data":[3,8,5,2,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["15.79","42.11","26.32","10.53","5.26"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Journal of Fungi","Journal of Invertebrate Pathology","Virulence","Microbial Pathogenesis","Pathogens","Archives of Toxicology","Parasites and Vectors","ISME Journal","Folia Microbiologica","Biological Control","Developmental and Comparative Immunology","Food and Chemical Toxicology","Entomologia Experimentalis et Applicata","Critical Reviews in Biotechnology","Fungal Biology"],"ids":[3021,10098,17735,8685,20044,21003,17499,17218,11559,7311,11812,8445,9576,19934,11372],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[2,2,2,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[10.53,10.53,10.53,5.26,5.26,5.26,5.26,5.26,5.26,5.26,5.26,5.26,5.26,5.26,5.26],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Springer Nature","Multidisciplinary Digital Publishing Institute (MDPI)","Taylor & Francis","Wiley"],"ids":[17,8,202,18,11],"codes":[0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp"],"datasets":[{"label":"","data":[7,4,3,3,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[36.84,21.05,15.79,15.79,5.26],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Grizanova E. V. et al. Metarhizium brunneum infection dynamics differ at the cuticle interface of susceptible and tolerant morphs of Galleria mellonella // Virulence. 2019. Vol. 10. No. 1. pp. 999-1012.
GOST all authors (up to 50)
Copy
Grizanova E. V., Coates C., Dubovskiy I. M., BUTT T. M. Metarhizium brunneum infection dynamics differ at the cuticle interface of susceptible and tolerant morphs of Galleria mellonella // Virulence. 2019. Vol. 10. No. 1. pp. 999-1012.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1080/21505594.2019.1693230
UR - https://doi.org/10.1080%2F21505594.2019.1693230
TI - Metarhizium brunneum infection dynamics differ at the cuticle interface of susceptible and tolerant morphs of Galleria mellonella
T2 - Virulence
AU - Grizanova, Ekaterina V
AU - Coates, Christopher
AU - Dubovskiy, Ivan M.
AU - BUTT, T. M.
PY - 2019
DA - 2019/01/01 00:00:00
PB - Taylor & Francis
SP - 999-1012
IS - 1
VL - 10
PMID - 31724467
SN - 2150-5594
SN - 2150-5608
ER -
Cite this
BibTex
Copy
@article{2019_Grizanova,
author = {Ekaterina V Grizanova and Christopher Coates and Ivan M. Dubovskiy and T. M. BUTT},
title = {Metarhizium brunneum infection dynamics differ at the cuticle interface of susceptible and tolerant morphs of Galleria mellonella},
journal = {Virulence},
year = {2019},
volume = {10},
publisher = {Taylor & Francis},
month = {jan},
url = {https://doi.org/10.1080%2F21505594.2019.1693230},
number = {1},
pages = {999--1012},
doi = {10.1080/21505594.2019.1693230}
}
Cite this
MLA
Copy
Grizanova, Ekaterina V., et al. “Metarhizium brunneum infection dynamics differ at the cuticle interface of susceptible and tolerant morphs of Galleria mellonella.” Virulence, vol. 10, no. 1, Jan. 2019, pp. 999-1012. https://doi.org/10.1080%2F21505594.2019.1693230.